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Plasmatech - Nanoforum 2013 - Ultra-pure nanoparticles

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PlasmaTech - a start-up company of the University of Pisa - presents the last ultra-pure metal nanoparticles by laser ablation for nanotechnology applications: from nanobiotechnology to advanced materials

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Plasmatech - Nanoforum 2013 - Ultra-pure nanoparticles

  1. 1. NANOFORUM 2013 – Ing.Alessandro Balvis – info@plasmatech.it
  2. 2. System NPs Properties Custom PartnersIntroduction MISSION Solutions from laser/matter interaction for different application fields Spectroscopy • spectroscopy and absorption techniques for material characterization (high- and low-resolution spectroscopy systems) Plasma Diagnostics • gas/plasma analysis and diagnostics (dispersion interferometer) Nanoparticles • metallic nanoparticles production using laser ablation techniques PROFILE  start-up company of the University of Pisa  established in 2012  developed by the scientific group “Intense laser fields and collective effects in plasmas” run by Professor F.Giammanco in the Department of Physics
  3. 3. WHY NANOPARTICLES? The NABLA (Nanoparticelle Metalliche Prodotte per Ablazione Laser) project • Regione Toscana - PAR FAS Linea di azione 1.1.a.3 – 2011/2013 • Partners o Dipartimento di Fisica “E.Fermi” - Università di Pisa (UNIPI) o Istituto Sistemi Complessi del CNR (ISC) – Sesto Fiorentino (FI) o Dipartimento di Chimica “U.Schiff” - Università di Firenze (UNIFI) Objectives of the project • characterization of the production process of metal nanoparticles for laser ablation • refinement of the process of reshaping with laser irradiation on nanoparticles • bimetallic nanoparticles production and nanostructured surfaces for Raman diagnostics System NPs Properties Custom PartnersIntroduction
  4. 4. Introduction NPs are produced by the use of short pulses of laser energy focused on a target in a solvent . We can obtain ultra-pure colloids free of surfactants and/or reaction products. Changing the system parameters as laser spot diameter and laser energy, we can change the NPs properties. NPs Properties Custom PartnersSystem • metallic target in liquid environment • pico-second Nd-YAG laser • 1,064/532 nm wavelength • on-line spectrum measurement • production rate: ~ 20cc/h
  5. 5. Introduction System Custom PartnersNPs Properties TARGET SOLVENT OPT. STABILIZER QUALITY STANDARD Au WATER ETHANOL ACETONE METHANOL PAMAM PEG HEC ABS. SPECTRUM ZETA POTENTIAL TEM DLS Ag IN PROGRESS Ti Pd Cu We defined protocols for nanoparticles production in different solvents with the common quality measurements • STANDARD - Au/Ag nanoparticles - dimension <10nm – high-stability (6-12 months) • IN PROGRESS - Ti/Pd/Cu nanoparticles - dimension <10nm
  6. 6. Introduction System Custom PartnersNPs Properties GOLD - Specifications Dispersion medium Water Average particle size 6 nm Average size tolerance +: 4.14 nm _: 2.48 nm Zeta Potenzial -50 mV Optical Density (OD) 1 Volume 50-100 ml Surfactant None 0 5 10 15 20 2 7 12 17 22 29 37 Relativefrequancy(%) Diameter [nm] SILVER - Specifications Dispersion medium Water Average particle size 3 nm Average size tolerance +:2,29 nm -: 1,25 nm Zeta Potenzial -45 mV Optical Density (OD) 1 Volume 50-100 ml Surfactant None 0 5 10 15 20 25 30 1 4 7 10 13 17 20 RelativeFrequency(%) Diameter [mm]
  7. 7. Introduction System Custom PartnersNPs Properties NPs Nanoparticles in HEC (Hydroxyethyl cellulose) Powder NPs Drying Main advantages • NPs are re-dispersible • long term stability • same spectrum features before/after drying • easy-to-transport DEMO SAMPLES AVAILABLE AT THE STAND HEC powder
  8. 8. CustomNPs PropertiesIntroduction System Partners Plasmatech can also offer special nanoparticles and nanostructures: bimetallic by laser ablation and nanocages/nanostars by chemical reduction SHAPE MATERIAL SOLVENT DIM. FIELDS Bimetallic Cu/Ag Water 2  10 nm SERS, catalysis, enhanced bactericidal effect, hydrogen storage Cu/Pd Ag/Pd Nanocage 1) Au 50 ÷ 70 nm photoacoustic tomography, drug deliveryAg Nanostar 2) Au 50 ÷ 500 nm SERS, enhanced IR spectroscopy 1) chitosan capped or fluorophore- functionalized 2) CTAB capped, unprotected, fluorophore-functionalized
  9. 9. NPs PropertiesIntroduction System Custom Partners MEDICAL GENETICS UNIVERSITY OF PISA PSG INSTITUTE OF ADVANCED STUDIES (COIMBATORE, INDIA) «RASPERI» PROJECT Gold NPs by laser ablation: first cytotoxicity and genotoxicity studies Gold NPs for CA 15.3 antibody tagging studies Gold NPs for biosensor: rapid detection of urinary tract infections • Unprotected NPs in pure water • Tests on their toxicity are not yet available • Preliminary experiments: evaluate the effects of gold NPs obtained by laser ablation in pure solvents and in different experimental conditions • Gold NPs by laser ablation • CA 15.3 functionalization • Raman measurements and spectroscopy studies • fast and reliable UTI diagnosis based on Surface Enhanced Raman Spectroscopy • special nanoparticles- based surfaces for SERS measurements
  10. 10. NANOFORUM 2013 STAND POP-B

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