The Microscope


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In this PowerPoint Presentation, you will learn all about the microscope, its parts and functions.

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The Microscope

  1. 1. The Microscope
  2. 2. What structure in an animal’s body is this?Where are these found?
  3. 3. How do biologists study cells?
  4. 4. A microscope is an instrument that produces magnified images of tiny structures.
  5. 5. Early Microscope
  6. 6. Early Microscope
  7. 7. Modern Microscopes
  8. 8. Compound Light Microscope  contains more than one lens  magnification: up to 1000x  useful for studying many kinds of cells and small organisms
  9. 9. Electron Microscope in the 1920s, a German physicist discovered a way to use magnets to focus beam of electrons uses a beam of electrons instead of light to examine a sample magnification: as much as 1000x larger than a light microscope
  10. 10. Electron Microscope Two Kinds:  Transmission Electron Microscope (TEM)  Scanning Electron Microscope (SEM)
  11. 11. TEM  gives a 2 - D view  works much like a slide projector  specimen must be cut into very thin slices so that the electron beam can pass through
  12. 12. SEM  gives a 3 - D view  uses a thin beam of electrons to scan a sample’s surface  does not reveal the internal structure of a sample
  13. 13. Images produced by light and electronmicroscope
  14. 14. Terms to remember:Magnification – ability of the microscope to magnify or enlarge an object (example: 5x, 10x, 45x)Total Magnification: magnification of the eyepiece x magnification of the objectiveResolution = sharpness of an image
  15. 15. Parts of the Microscope
  16. 16. Parts of the MicroscopeThree main parts:A. Optical PartsB. Illuminating PartsC. Mechanical Parts
  17. 17. A. Optical Parts- used for magnification of the image 1. Ocular / eyepiece 2. Objectives
  18. 18. A. Optical PartsObjectives – tubes attached to the revolving nosepieceA. SPO (4x) – gives an overview of the specimenB. LPO (10x) – used for locating parts of the specimenC. HPO (40x) – use to view small details of the specimen
  19. 19. B. Illuminating Parts- used to focus or direct light1. Mirror - used to reflect light - plane, convex2. Diaphragm - regulates the amount oflight reflected
  20. 20. C. Mechanical Parts- necessary for movement and support1. Base – allows the microscope to stand2. Pillar – supports the stage3. Stage – where glass slide is placed4. Arm – curved part grasped whencarrying the microscope
  21. 21. C. Mechanical Parts5. Revolving nosepiece – where theobjectives are attached6. Draw tube – holds the eyepiece7. Coarse adjustment knob – raises orlowers the body tube to bring image intofocus8. Fine adjustment knob – focuses imagefurther
  22. 22. C. Mechanical Parts9. Inclination joint – allows tilting of themicroscope10. Mirror rack – holds mirror in place11. Stage clip – holds specimen in placeon the stage