Nick’s Test
Last Saturday… <ul><li>Nick and his group “Neisseria” were playing at the battle of the bands competition. </li></ul><ul><...
Neiserria received an encore… <ul><li>Nick was so excited that he forgot to collect his water bottle… </li></ul><ul><li>He...
When he returned… <ul><li>There were several water bottles on stage so Nick just grabbed the one he thought was his… </li>...
Several days later… <ul><li>Nick is feeling sick. </li></ul><ul><li>He is vomiting </li></ul><ul><li>Has a temperature </l...
He goes to the doctor… <ul><li>Who immediately orders a test for meningitis… </li></ul><ul><li>You are the scientist </li>...
Prepare gold nanoparticles by adding antibodies… <ul><li>Antibodies can be glued onto the nanoparticles.  These will stick...
Click to add antibodies… Y Y Y Y
How it works… <ul><li>When the disease protein (green star) is present, the nanoparticles clump together. </li></ul><ul><l...
Click to add the Nanoparticles
Let’s do multiple tests
To check that the test is working.. <ul><li>We need some controls… </li></ul><ul><li>Add Nanoparticles to the remaining we...
Now for the samples… <ul><li>First add the positive control-  this should change colour because  </li></ul><ul><li>it cont...
Let’s repeat the test… <ul><li>The positive control is working properly. </li></ul>
Then add the negative control… <ul><li>the negative control should not cause a reaction because there are no meningitis pr...
Let’s repeat the test… <ul><li>The negative control is working effectively. </li></ul>
Now let’s test Nick’s sample <ul><li>The sample is negative… </li></ul>+ - Nick
We had better repeat the test <ul><li>The other samples are positive. </li></ul>+ - Nick Luckily we repeated the test!
Nick was placed onto intravenous antibiotic therapy…
As a precaution his band and his whole family was treated and immunised.. <ul><li>Even his annoying little sister … </li><...
Nick made a full recovery… <ul><li>He still plays with his band… </li></ul><ul><li>And he is still constantly irritated by...
Questions <ul><li>What is bacterial meningitis? </li></ul><ul><li>How did Nick contract the disease? </li></ul><ul><li>Why...
Upcoming SlideShare
Loading in …5
×

Health & Medicine - NanoGold Activity2 Nicks Test

975 views

Published on

Published in: Health & Medicine, Technology
  • Be the first to comment

  • Be the first to like this

Health & Medicine - NanoGold Activity2 Nicks Test

  1. 1. Nick’s Test
  2. 2. Last Saturday… <ul><li>Nick and his group “Neisseria” were playing at the battle of the bands competition. </li></ul><ul><li>He left his water bottle at the edge of the stage. </li></ul>
  3. 3. Neiserria received an encore… <ul><li>Nick was so excited that he forgot to collect his water bottle… </li></ul><ul><li>He had to wait until the interval. </li></ul>
  4. 4. When he returned… <ul><li>There were several water bottles on stage so Nick just grabbed the one he thought was his… </li></ul><ul><li>He was so thirsty he took a big drink… </li></ul>
  5. 5. Several days later… <ul><li>Nick is feeling sick. </li></ul><ul><li>He is vomiting </li></ul><ul><li>Has a temperature </li></ul><ul><li>And a very stiff neck </li></ul>
  6. 6. He goes to the doctor… <ul><li>Who immediately orders a test for meningitis… </li></ul><ul><li>You are the scientist </li></ul><ul><li>Complete the diagnostic </li></ul><ul><li>test to discover if Nick has </li></ul><ul><li>Meningitis. </li></ul>
  7. 7. Prepare gold nanoparticles by adding antibodies… <ul><li>Antibodies can be glued onto the nanoparticles. These will stick directly to proteins on the meningitis bacteria if Nick has the disease. </li></ul>Gold Nano Particle Y Y Y Antibodies Y
  8. 8. Click to add antibodies… Y Y Y Y
  9. 9. How it works… <ul><li>When the disease protein (green star) is present, the nanoparticles clump together. </li></ul><ul><li>There is a colour change from red to blue. </li></ul>Y Y
  10. 10. Click to add the Nanoparticles
  11. 11. Let’s do multiple tests
  12. 12. To check that the test is working.. <ul><li>We need some controls… </li></ul><ul><li>Add Nanoparticles to the remaining wells.. </li></ul>
  13. 13. Now for the samples… <ul><li>First add the positive control- this should change colour because </li></ul><ul><li>it contains meningitis protein. </li></ul>Pipette image: Microsoft Clipart
  14. 14. Let’s repeat the test… <ul><li>The positive control is working properly. </li></ul>
  15. 15. Then add the negative control… <ul><li>the negative control should not cause a reaction because there are no meningitis proteins present. </li></ul>
  16. 16. Let’s repeat the test… <ul><li>The negative control is working effectively. </li></ul>
  17. 17. Now let’s test Nick’s sample <ul><li>The sample is negative… </li></ul>+ - Nick
  18. 18. We had better repeat the test <ul><li>The other samples are positive. </li></ul>+ - Nick Luckily we repeated the test!
  19. 19. Nick was placed onto intravenous antibiotic therapy…
  20. 20. As a precaution his band and his whole family was treated and immunised.. <ul><li>Even his annoying little sister … </li></ul><ul><li>Who can play the triangle </li></ul><ul><li>And can’t wait to join the band! </li></ul>
  21. 21. Nick made a full recovery… <ul><li>He still plays with his band… </li></ul><ul><li>And he is still constantly irritated by his kid sister. </li></ul><ul><li>Nick was very lucky to be treated so promptly thanks to your early diagnosis using nanotechnology. </li></ul>
  22. 22. Questions <ul><li>What is bacterial meningitis? </li></ul><ul><li>How did Nick contract the disease? </li></ul><ul><li>Why was it so important that Nick be diagnosed early? </li></ul><ul><li>What are some of the serious implications of having this disease? </li></ul><ul><li>Explain what is meant by a positive and a negative control. </li></ul><ul><li>Why do scientists repeat their experiments? </li></ul>

×