Image from http://en.wikipedia.org/wiki/Cancer, image uploaded by http://de.wikipedia.org/wiki/Benutzer:Lange123 Beschreibung: Konventionelles Röntgenbild des Thorax (der Lunge) mit rundlicher Verdichtung in der linken Lunge Quelle: selbst erstellt -- de:Benutzer:Lange123 17:18, 11. Nov. 2004 (CEST)
Infection stage. Slide shows the bacteria as yellow spheres. The blue indicates a mucosal surface in a human. The long hairy structures are called cilia which are part of cells that help move particles over their surface. Image: http://www.pbs.org/wgbh/nova/meningitis/bloodstream.html
A toxin is a poison. Meningitis bacteria produce large amounts of poison. Image: http://www.pbs.org/wgbh/nova/meningitis/bloodstream.html
How the poison causes damage to the infected person. The picture indicates a ruptured blood vessel. Image: http://www.pbs.org/wgbh/nova/meningitis/bloodstream.html
Image of Japanese Yakult from http://en.wikipedia.org/wiki/Image:Yakult_(Yakult_Honsha).jpg
The ultra-sensitive test mentioned in the slide is the biobarcode method for detecting DNA – see animation.
http://www.dorlingkindersley-uk.co.uk Reminder of “what if” scenario. The following slides will discuss various nanoparticles that could be used as alternatives to surgery, particularly for invasive types of cancer that effects vital organs like the brain. Sometimes surgery may mean survival but a loss of function, e.g. paralysis. A person who has cancer in an organ is not eligible for organ donation. Therefore it cannot be removed and replaced with a new organ.
http://www.flickr.com/photos/iurifernandes/2465538344/ Nano bullets. Use of coated gold nanoparticles that when heated preferentially kill cancer cells.
Australian example info found at: http://www.dest.gov.au/sectors/science_innovation/science_agencies_committees/prime_ministers_science_engineering_innovation_council/meetings/thirteenth_meeting.htm
The idea is to detect a protein that is only found in the disease state. Two different types of nanoparticle are used. The gold nanoparticle has antibodies on its surface that detect the disease protein. The gold nanoparticle also has DNA fragments on it. The second particle used is magnetic. It has antibodies to the disease protein on its surface. If the disease protein is present, both the gold nanoparticle and the magnetic nanoparticle will bind to it. This will create a sandwich effect.
Image courtesy of http://www.flickr.com/photos/mujitra/2559450853/ (creative commons license)
Photos and info sourced from nanoprobes, http://www.nanoprobes.com/
Photos and info sourced from nanoprobes, http://www.nanoprobes.com/
Introductory slide showing a “nanobot” the size of a red blood cell. Of course this would make the bot actually a microsized bot. Nevertheless the components inside the bot must be nanosized.
Health & Medicine Module - NanoGold
Health & Medicine Nanogold
Module Outcomes <ul><li>Understand the importance of developing a rapid diagnostic test for meningitis </li></ul><ul><li>Understand how nanotechnology can improve the sensitivity of medical diagnostics </li></ul><ul><li>Be familiar with the characteristics of nanoparticulate gold </li></ul><ul><li>Identify recent developments in nanotechnology and how they may benefit human health. </li></ul>
What If? Image: bbaunach@flickr What if you could save a baby’s life with a rapid test for meningococcal disease?
What If? Image: Benutzer:Lange123@wikipedia What if you could selectively kill cancer cells and not surrounding healthy tissue? Lung cancer
Activity 1 The following is a headline from ABC news online, August 13 2008: ‘ A 14 year-old boy has died from a suspected case of meningococcal disease on the New South Wales central coast .’ Perform the Meningitis Activity to learn more about this fatal disease.
Two Types Of Meningococcal Disease <ul><li>Meningitis - bacteria attack the meninges (lining around the brain) - early symptom is a stiff, sore neck - meninges and brain swell, putting pressure on nerves - survivors often left deaf or with brain damage </li></ul><ul><li>Sepsis - infection occurs throughout the bloodstream - affects the entire body - bacterial toxins rupture blood vessels and can rapidly shut down vital organs - survivors often left with permanent injuries and/or require amputations. </li></ul>
Not All Bacteria Is Bad Image: Yakult.Honsah@wikipedia <ul><li>Very few bacteria are harmful, most bacteria have the following functions: - Food production - Normally live on our bodies and stop pathogenic bacteria from growing - Help with digestion (hence dietary supplements) - Can be genetically altered to make the products we need in the laboratory e.g. vaccines, drugs. </li></ul>
Experiment 1 Observation Of Bacteria Perform the Observation of Bacteria experiment as instructed by your teacher.
How Can We Diagnose Meningitis? <ul><li>Current tests detect bacterial proteins in blood/other fluid - can only detect in the range of micro) mol (10 -6 ) to pico mol (10 -12 ) of protein concentration </li></ul><ul><li>If we develop new tests using gold nanoparticles combined with DNA amplification we could detect molecule concentrations in the atto (10 -18 ) mol range i.e. one million times more sensitive than current tests </li></ul><ul><li>This would allow earlier detection and therefore faster medical interventions. </li></ul>
What Are Gold Nanoparticles? Image: Northwestern University <ul><li>Gold nanoparticles (‘ nanogold ’) occur as clusters of gold atoms up to 100nm in diameter. </li></ul><ul><li>Nanogold has unusual visible properties because the particles are small enough to scatter visible light - in contrast, mass gold reflects light </li></ul><ul><li>Gold nanoparticles appear deep red to black in solution - colour depends on size of nanoparticles </li></ul><ul><li>The distance between particles also affects colour - surface plasmon resonance is the term used by nanotechnologists to describe this effect. </li></ul>5nm gold clusters Gold nanoparticle
Experiment 2 Making Gold Nanoparticles Perform or watch a demonstration of the Making Gold Nanoparticles Experiment to see the differing chromic properties of nanoparticulate gold for yourself.
Animation <ul><li>View the Nanoparticles Diagnostics Animation to better understand how gold nanoparticles are used to detect bacterial meningitis </li></ul><ul><li>Complete a flow chart outlining the key steps in the nanoparticle diagnostic test. </li></ul>
Activity 2 <ul><li>Use the Nick’s Test PowerPoint presentation provided by your teacher to model the use of a nanoparticle diagnostic test in real-life </li></ul><ul><li>Answer the questions on the last slide. </li></ul>
Rapid Diagnosis So nanogold can be used to rapidly diagnose meningitis… … what else can nanogold offer to the world of medicine?
Nanogold: ‘Nanobullets’ To Treat Cancer Image: iurifernandes@flickr <ul><li>Also called 'thermal scalpels‘ or ‘nano bullets’ </li></ul><ul><li>Particularly useful for small cancers that are embedded in vital tissues such as the brain </li></ul><ul><li>The nanoshells are silica coated with gold nanoparticles that binds them to cancer cells, and are then injected into tumours </li></ul><ul><li>Light from a near-infrared laser, which is harmless to healthy tissue, is absorbed by the nanoshells. The nanoshells heat up, destroying the tumour cells. </li></ul>
Nanogold: ‘Drug Smugglers’ To Treat Cancer Image: Courtesy E. Zubarev, Rice University <ul><li>Gold Nanoparticles coated with bristles of an anticancer drug, Taxol® </li></ul><ul><li>Travel inside cancer cells and bind to internal structures </li></ul><ul><li>Sabotages cell machinery preventing cell division. </li></ul>
Activity 3 & Experiment 3 Perform the Gold Nanobullets Activity and the Golden Cancer Treatment Experiment to better understand the benefits of using nanogold to treat cancer .
What If? What if you could detect disease at home with a ‘lab on a chip’? <ul><li>Nanoparticles are bound to antibodies that recognise a disease marker protein – a protein found only in a disease state, or at higher levels in a disease state </li></ul><ul><li>When the protein is present in a sample, extraction of the magnetic particles also pulls out the gold nanoparticles because the two are bound together </li></ul><ul><li>The DNA on the gold nanoparticle can be copied many times, so very small amounts of the original protein can be detected </li></ul><ul><li>Potentially, nanoparticles with different antibody and DNA fragment combinations could be used to search for multiple disease marker proteins at the one time (hence the 'biobarcode'). </li></ul>View the BioBarcode Animation
What If? What if you could rapidly sequence DNA on a space mission? NASA is working on a ‘Nanopore Sensor’ to rapidly sequence DNA - detect DNA of pathogenic bacteria or viruses in astronauts - sequence DNA of new life forms. Image: mujitra@flickr
Nano-Imaging In Australia <ul><li>Nanotechnology Victoria and RMIT Medical Radiations have a collaboration investigating the use of nanogold for combined X-Ray imaging and radiotherapy, with a focus on cancer detection and treatment. </li></ul>ureter Dr Jeanette Pritchard, Manager, Imaging Delivery & Sensing, Nanotechnology Victoria Ltd
Animation ureter View the Nano-imaging Animation .
What If? ureter <ul><li>A ‘nanobot’ is a robot constructed from nanoscale components </li></ul><ul><li>Nanobots could one day be injected into the body to treat diseases by delivering drugs or to perform surgery </li></ul><ul><li>Nanobots could also perform a surveillance function where they check that the body is healthy and detect diseases very early. </li></ul>
Nanobot Research In Australia <ul><li>Researchers led by Dr James Friend at the MicroNanoPhysics Research Laboratory at Monash University, Melbourne are developing robot-like components and motors at the micro- and nano-scales. </li></ul><ul><li>Watch the Micro-motor Animation to learn more </li></ul>ureter
Activity 4 Perform the Nanobots and Design Your Own Nanobot activities to explore this concept further.
Quantum Problems ureter <ul><li>Play Duckboy in Nanoland! www. sciencemuseum .org. uk /antenna/ nano / nanoland / nanoworld .asp </li></ul><ul><li>MURKY MANSION </li></ul><ul><li>STUCK WITH THE DUCK </li></ul><ul><li>STRANGE ATTRACTIONS </li></ul><ul><li>BROWNIAN BUMPERS </li></ul><ul><li>FUNHOUSE </li></ul><ul><li>Answer the questions in the Design Your Own Nanobot Activity which relate to this game. </li></ul>
Summary ureter <ul><li>Meningococcal meningitis is a deadly bacterial disease for which rapid diagnosis can be critical to prevent death and permanent side effects </li></ul><ul><li>Gold nanoparticles have been used by Australian scientists to develop a rapid and sensitive test for meningococcal meningitis </li></ul><ul><li>Gold nanoparticles are the focus of many health tools currently in development - targeted at prevention, diagnosis and treatment. </li></ul>
Revision <ul><li>What is bacterial meningitis and how do the bacteria cause the symptoms of the disease? </li></ul><ul><li>Why have scientists developed a rapid diagnostic test for meningococcal infection? </li></ul><ul><li>Describe one way that gold nanoparticles can be used to treat cancer. </li></ul><ul><li>Do you believe that nanobots will one day be a reality? What problems must nanobot designers overcome? </li></ul>