Gc ms applications

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Gc ms applications

  1. 1. GUIDED BY SUBMITTED BYMR. PRASHANT BENIWAL VIKRAMJEET SINGHASST. PROFESSOR
  2. 2. INTRODUCTIONHYPHENATED TECHNIQUESADVANTAGES1. Shorter analysis time2. Higher degree of automation3. Higher sample throughput4. Better reproducibility
  3. 3. TYPE OF DIFFERENT HYPHENATEDANALYTICAL TECHNIQUES Gas chromatography- Mass spectrometry (GC-MS) Gas chromatography-Infra red spectroscopy Gas chromatography- Infra red spectroscopy- Mass spectrometry Gas chromatography-Thin layer chromatography Liquid chromatography- Mass spectrometry Capillary electrophoresis- Mass spectrometry
  4. 4. GAS CHROMATOGRAPHY- MASSSPECTROMETRY (GC-MS) INTRODUCTION PRINCIPLE
  5. 5. GAS CHROMATOGRAPH – MASSSPECTROMETER INTERFACE Effusion Separator Jet/Orifice Separator Membrane Separator
  6. 6. GC- MS INSTRUMENTATION
  7. 7. GC-MS APPLICATIONS Metabolite profiling Detection of lipophilic compounds in diverse plant tissues Analysis of biologically important aromatic amines. Application to human dosimetry. For the sensitive and quantitative single-run analysis of acidic phytohormones. and related compounds, and its application to Arabidopsis thaliana Identification of volatile components For the determination of pyrethroid residues in vegetable samples Analysis of Pesticides in Foodstuffs Environmental and forensic applications Miscellaneous
  8. 8. Metabolite profiling Toxicity assessment/toxicology e.g. a specific lesion in liver or kidney Functional genomics Nutrigenomics: e.g. Arabidopsis thaliana leaf extracts
  9. 9. Detection of lipophilic compoundsin diverse plant tissues liphophilic profiling of red and green tomato
  10. 10. Analysis of biologically importantaromatic amines and Applicationto human dosimetrye.g. Exposure to 2, 4-difluoroaniline (DFA) was monitored by GC-MS of DFA adducts bound to hemoglobin (Hb)
  11. 11. For the analysis of acidic phytohormones and related compounds(i) Often, there is a highly local and controlled production of enzymes of phytohormone bio synthesis. Examples; ethylene biosynthetic enzymes in abscission zones Gibberellins biosynthetic enzymes in embryo s (seeds) or meristems.(ii) phytohormone production has unveiled an amazing degree of interactions
  12. 12. Identification of volatile componentsand For the determination ofpyrethroid residues in vegetablesamples volatile components in certified brand of origin (CBO) Rias Baixas white wines
  13. 13. Environmental and forensic applicationsTypes of analysis systems : photo ionization detectors (PIDs) with and without a GC electrochemical and catalytic bead gas detection systems colorimetric tube systems ion mobility based systems (IMS) GC-MS
  14. 14. Miscellaneous Environmental monitoring and cleanup Criminal forensics Law enforcement Sports anti-doping analysis Security Food, beverage and perfume analysis Medicine

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