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Transgenic organisms powerpoint

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Transgenic organisms powerpoint

  1. 1. By Stephanie Wilson
  2. 2. How do you create a Transgenic Mouse?•Several methods but one most often used: Transgenic Mouse Process Animation
  3. 3. First Method of Transgenic Mice Creation• (basic steps) Standard Transgenic Approach: 1. Microinject fertilized mouse eggs with transgene
  4. 4. Methods continued…2. Injected eggs are implanted into a fostermother
  5. 5. Methods continued…3. Offspring are then born “Pups”; possiblefounders (have the transgene)
  6. 6. Method Continued…4. Potential founders (pups with the transgene) are found using a PCR analysis and or southern blotting (Black offspring = founders)5. Founders can then be further analyzed for affectsof that transgene
  7. 7. Methods Continue… Transgenic Mice Technology Part 1 Transgenic Mice Technology Part 2
  8. 8. Why create transgenic mice?•Transgenic Mice have: •Aided the study of Alchemies Disease • Aided in study of pancreatic cancer Transgenic Mice aids study of Pancreatic Cancer •Acted as disease models •Tested toxicants •Produced specific proteins helpful in biotechnology
  9. 9. Pros and Cons• Pros – Cheap – Short generation time – Defined genes• Cons – Ethical Concerns • Are the animals treated with respect? – Environmental Issues • What if a genetically modified animal gets loose? – Religious Views • Do we have the right to act as God?
  10. 10. Animation And Picture Credits• Experiments." Experiments. Web. 23 May 2012. <http://student.biology.arizona.edu/honors2005/group07/Experiments.html>.• "Transgenic Mice." Transgenic Mice. Web. 23 May 2012. <http://www.accessexcellence.org/RC/VL/GG/transgenic.php>.• Creating Transgenic Mice: On-Line Self-Study." Human Development. Professor Danton ODay. Web. <http://www.utm.utoronto.ca/~w3bio380/pdf/Creating%20Transgenic%20Mice.pdf>.• "Transgenic Mouse Animation." Transgenic Mouse Animation. Web. 23 May 2012. <http://spine.rutgers.edu/cellbio/assets/flash/transgen.htm>.• Transgenic Animals. 13 June 2011. Web. <http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html>.• "Fig002jme: Two Methods to Produce Transgenic Mice." Fig002jme: Two Methods to Produce Transgenic Mice. Web. 23 May 2012. <http://journals.cambridge.org/fulltext_content/ERM/ERM3_12/S1462399401002952sup00 4.htm>.• Proneural. "The Science of Transgenic Technology Part 2 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=EFNtTfhhQ70>.• Proneural. "The Science of Transgenic Technology Part 1 of 2." YouTube. YouTube, 25 Apr. 2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=ujZHrR1mro8>.

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