LAB GUIDE ON SKIN 2005.PDF

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LAB GUIDE ON SKIN 2005.PDF

  1. 1. SKIN HISTOLOGY: UP CLOSE AND PERSONAL A Laboratory Guide
  2. 2. SLIDES TO CONQUER • 56a: Thin skin, H & E • 56b: Thick skin, Masson, H & E • 56e: Scalp • 56f: Pacinian corpuscle • 56g: Meissner corpuscle
  3. 3. KNOW THE HIERARCHY OF HISTOLOGIC ORGANIZATION CELLS (Keratinocytes, etc.) + ECM TISSUE Epithelial tissue (= EPIDERMIS) Connective tissue (=DERMIS/HYPODERMIS) Nervous tissue Muscle tissue Blood tissue ORGAN Skin
  4. 4. SKIN (Organ) EPITHELIAL TISSUE NERVOUS (=Epidermis, CONNECTIVE BLOOD MUSCLE TISSUE TISSUE glands, TISSUE TISSUE endothelium (=Dermis, (=Free nerve hypodermis) (=Smooth of blood endings, muscle cells in vessels) encapsulated blood vessel receptors, wall, platysma, Merkel cell) Arrector pili)
  5. 5. • EPITHELIAL TISSUE • CONNECTIVE TISSUE – CELLS CLOSELY – CELLS FEW AND FAR PACKED TOGETHER BETWEEN W/ INTERCELLULAR – ABUNDANT JUNCTIONS EXTRACELLULAR – SCANTY MATRIX EXTRACELLULAR • FIBERS MATRIX • GROUND SUBSTANCE – AVASCULAR – VASCULAR – GLANDS *INTIMATE RELATIONSHIP - ATTACHED TOGETHER BY A BASEMENT MEMBRANE
  6. 6. ORIENT THE SLIDE PROPERLY! X
  7. 7. RECOGNIZE THE TISSUE • EPIDERMIS – Stratified squamous COMPONENTS THAT keratinized epithelium – Superficial MAKE UP THE SKIN – Thinner • DERMIS – Dense irregular connective tissue – Thicker • EPIDERMAL-DERMAL INTERFACE – Wavy – Basement membrane not visible w/ H & E • HYPODERMIS – Loose connective tissue – May have abundant adipose cells
  8. 8. IDENTIFY THE LAYERS • EPIDERMIS – More deeply staining – Highly cellular (multiple layers) • DERMIS – Paler – Mostly Fibers (irregular bundles) and some ground substance (poorly stained w/ H & E)
  9. 9. ARTEFACT! • ISLANDS OF DERMAL PAPILLA WITHIN THE EPIDERMIS (due to tangential sectioning)
  10. 10. THIN SKIN VS THICK SKIN Slide 56a Slide 56b
  11. 11. LAYERS OF THE EPIDERMIS (thick skin) • S. corneum • S. granulosum • S. spinosum • S. basale
  12. 12. • S. corneum – Anucleate – flattened layers – “basketweave” • S. granulosum – Diamond shaped – Darkly staining cytoplasmic granules • S. spinosum – Polygonal – Rarely see “spines” • S. basale – Single layer, cuboidal cells – Crowded nuclei – Occ. Mitotic figures
  13. 13. MELANOCYTE (thin fair skin)
  14. 14. MELANOCYTE (Pigmented skin)
  15. 15. LANGERHANS CELL
  16. 16. LAYERS OF THE DERMIS • Papillary dermis – Contains dermal papilla – Thin collagen fibers – More cells (fibroblasts) – Smaller blood vessels (capillaries) • Reticular dermis – Thick collagen fibers – Bigger blood vessels (arterioles/venules) – *Elastic network NOT distinguished from collagen with H&E
  17. 17. VELLUS vs TERMINAL (most of body) (scalp) • Superficial • Deeper • Smaller • Bigger • Lightly pigmented • Darkly pigmented
  18. 18. Slide 56e: Scalp • HAIR FOLLICLES – Terminal (up to hypodermis) – Various planes of section – Mostly anagen – Oblique – Continuous with the surface epidermis
  19. 19. ANAGEN HAIR FOLLICLE • Hair shaft • Hair bulb • Hair matrix • Hair papilla
  20. 20. LAYERS OF THE HAIR FOLLICLE: Longitudinal section • Hair shaft • Inner root sheath • Outer root sheath • Fibrous sheath
  21. 21. LAYERS OF THE HAIR FOLLICLE: Cross Section
  22. 22. PILOSEBACEOUS UNIT (Longitudinal section) • Arrector pili muscle – Attaches to lower third of HF • Sebaceous gland – Duct enters into upper third of HF
  23. 23. PILOSEBACEOUS UNIT: Cross section • Hair Follicle • Sebaceous gland lobules
  24. 24. SEBACEOUS GLAND • CENTRAL: Vacuolated lipid-filled cells w/ scalloped nuclei • eventually disintegrating together with sebum into duct • PERIPHERAL: row of flattened germinative cells w/ large nuclei & basophilic cytoplasm
  25. 25. ECCRINE GLAND: LPO Coiled portion in deep dermis
  26. 26. ECCRINE GLAND: Coiled portion in deep dermis (HPO) • SECRETORY PORTION – Pale – More cross sectional profiles, some tangential and bizarre-shaped (=tadpole) • DUCTAL PORTION – Dark – Fewer, more regularly rounded profiles
  27. 27. ECCRINE GLAND: Coiled basal portion • Secretory portion – Single layer – Pale pyramidal cells • Ductal portion – 2 layers – Eosinophilic cuboidal cells – Hyaline lumenal lining
  28. 28. INTRAEPIDERMAL ECCRINE DUCT (=Acrosyringium) • Corkscrew appearance • Ductal cells are also keratinized
  29. 29. MEISSNER PACINIAN CORPUSCLE (56g) CORPUSCLE (56f)
  30. 30. MEISSNER vs. PACINIAN CORPUSCLE CORPUSCLE
  31. 31. BLOOD VESSELS in Reticular dermis • Arterioles • Venules
  32. 32. Capillaries in Dermal papilla MEISSNER’S CORPUSCLE LYMPHATIC VESSEL

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