Uses small pieces of DNA to identify: Criminals Parental Identity Bodies Most commonly used for court cases where DNA is used as evidence
DNA is unique to its person-much like fingerprints Chances that you will find two people with the same DNA who are not identical twins is VERY low DNA is extremely reliable in identifying who a person is, much like a fingerprint can identify who someone is DNA is 99.9% similar between humans!
There are 6 steps to fingerprinting: 1. Isolation of DNA 2. Cutting, sizing, and sorting of DNA 3. Transfer of DNA to nylon 4. Probing 5. Probing 6. DNA Fingerprint
**NOTE: THIS PROCESS WAS MADE TO DO AT HOME** Obtain a sample of DNA from mouth, and spit into a shot glass Add 3 pinches of table salt Add 1 mL. of dish soap-stir for 3 minutes Do not make bubbles Pour 5 mL. of alcohol down the glass’s side Wait for 5 minutes DNA will become visible, can be extracted with toothpick
Restriction enzymes cut DNA DNA then sorted by size using electrophoresis DNA placed in gel, then electric current used to drag pieces through the gel Gel is made from seaweed agarose
Gel used in electrophoresis is placed on nylon Allowed to sit and dry overnight, so that all liquid is evaporated, and only DNA is left
Use restriction enzymes to cut DNA Add radioactive N-bases to DNA Pol, which are coded for a wanted gene Once the DNA Pol has synthesized a new DNA strand, the radioactive labels will be able to be read!
The DNA fingerprint can now be assembled Have the radioactive labels transfer to the sheet Align the genetic information with that of the criminal/suspect You are now ready to catch a criminal!