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4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
4 Ppt Dengue By Maria Niaz
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4 Ppt Dengue By Maria Niaz

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Transcript

  • 1.  
  • 2. D E N G U E MARIA NIAZ BS.MT 4 TH SEMISTER
  • 3.  
  • 4. Introduction
    • Dengue is a virus spread by Aedes mosquitoes that bite during the day.
    • Unfortunately, there’s no vaccine to prevent it.
    • The best you can do is avoid being bitten.
  • 5. Pathogenecity
    • Dengue is a virus serotypes DEN-1, DEN-2, DEN-3, DEN-4, cause dengue (DEN) and dengue haemorrhagic fever (DHF)
    • These viruses are transmitted by Aedes mosquities
  • 6. Dengue
    • Characterized by;
    • * High fever
    • * Headache
    • * Pain behind the eyes
    • * Pain in muscles
    • * Joints pain
    • * Enlarged lymph glands
    • * Maculopapular rash
  • 7.  
  • 8. Contd………
    • Sometimes ;
    • * Abdominal pain
    • * Vomiting
    • Occasionaly ;
    • * Haemorrhagic symptoms
  • 9. Contd…………
    • Virus enter into the blood circulation by multiplicatoin of virus in the reticuloendothelial system
    • Leucopenia is the most infectious cause
  • 10.  
  • 11. Contd……………
    • Early infections are misdiagnosed by ;
    • * malaria
    • * hepatitis
    • * flu-like illness
    • High infectious rates occur in epidemics (70%-80%) of populations can de affected
  • 12. Dengue haemorrhagic fever
    • Serious life-threatening condition
    • Frequently children and others are affected whom previously infected with one serotype or strain of dengue virus and subsequently become reinfected with a different serotype or strain
    • The secondary immue response leads to the formation of virus-antibody immune complexes which activate complement
  • 13. Contd………
    • These complements are causing vascular damage and dissaminated intravascular coagulation (DIC) with spontaneous bleeding
    • In some patients, DHF progresses to circulatory collapse, referred as dengue shock syndrome (DSS)
  • 14. Contd………..
    • DHF is characterized by;
    • Raised haematocrit (PCV) due to plasma leakage
    • Redused platelet count
    • Hepatomegaly
    • Haemorrhagic symptoms with bleeding into the skin
  • 15. Dengue strikes
  • 16. Go for performing dengue tests in serology laboratory of Liaquat National Hospital
  • 17. Dengue test
    • Dengue test performe by the immunochromatographic method using the BIAS-3 (bi-directional immuno assay system) dengue IgG/IgM kit use in LNH microbiology lab
    • BIAS-3; two step assay for the differential detection of IgG or IgM antibodies to dengue virus in serum or whole blood
    • Use for in vitro diagnosis
  • 18. Principle of the test
    • This test is the membrane based screening test to differentialy detect the presence of antibodies to dengue virus
    • Lateral flow immunochromatographic type assay
    • Once the sample is added to the sample pad along with diluent the mixture passes bi-directionally through two antibody binding complexes which then binds the imunoglobulins in the sample
  • 19. Contd………..
    • The complex passes over the immobilized antigens on the membrane, if any antibodies to dengue (IgG or IgM) are present the antigens capture them in turn
    • This produces a pink/purple band in the test-zone of the device
    • The remaining complex continues to migrate to a control area on the test device and produces a pink/purple band each control area
    • This control bands indicate that the test has been performed properly
  • 20. Kit components
    • BIAS-3 Dengue IgG/IgM test device
    • Diluent in dropper vial
    • Directions for use
  • 21. Dengue rapid test device
  • 22. Stability and Storage Conditions
    • The BIAS-3 Dengue IgG/IgM test kit is stable at any room temperature between 8-30C when in the unopened pouches.
    • DO NOT FREEZE the kit or expose to temperature extremes.
    • Stability of the kit is 24 months from the date of manufacture - dating is indication on the kit label.
  • 23. General Precautions
    • The test is for In Vitro Diagnostic Use only
    • Appropriate infection control and handling procedures should be followed - do not smoke, eat or drink in the area where the test is to be performed. Use suitable clothing and gloves when handling samples and when performing the test
  • 24. Sample Collection
    • The BIAS-3 Dengue IgG/IgM test can be run on serum or whole blood
    • The test works best on fresh samples.
    • If testing cannot be done immediately, they should be stored at 2-8C after collection for up to 3 days
    • If testing cannot be done within 3 days, serum can be stored frozen at -20C or colder
  • 25. Test Procedure
    • For WHOLE BLOOD - add 10 uls of sample to the center sample pad hole (S) of the test device using a measuring pipet.
    • For SERUM - add 5 uls of sample to the center sample pad hole of the test device using a measuring pipet.
  • 26. Contd…………
    • Follow sample addition with 6drops of the diluent provided in the dropper bottle by holding the bottle vertically over the hole in the center sample pad cover. SLOWLY add diluent 1-2 drops at a time waiting for the previous drops to absorb
    • Results are then read in as little as 5minutes for strong positives or upto 30minutes for weaker positives and to make sure negatives are confirmed
  • 27. Reading the Test Results
    • Negative Only one pink/purple band appears in the Control areas of the BIAS test device.
  • 28. Contd………
    • Positive One or Two pink/purple bands appear in the Test area of the BIAS test device and one line each in the Control area of the test device
  • 29. Quality Control
    • It is recommended that a known positive and negative control be run to insure proper performance
    • All controls should be handled in the same manner as patient samples
  • 30.  
  • 31. T H A N K Y O U

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