Analysis of Vitamin C (ascorbic acid) in fruit juices of the same brand (Peel Fresh)
CHEMISTRY COLLOQUIUM2013Analysis of vitamin c(ascorbic acid) in fruit juicesName: Ysabel DeborahTeacher advisor: Pn Betty Wong
To determine the content ofvitamin c (ascorbic acid) indifferent types of fruits juicesTo investigate the importanceof vitamin c (ascorbic acid) tohuman bodyTo investigate the deficiencyof vitamin c (ascorbic acid) inthe human body
Vitamin C ( Ascorbic Acid)Structure of vitamin c/ ascorbic acid
Do different fruit juices of thesame brand contain the samecontent of vitamin c?
The pH value of the different fruitjuices from same brands wasmeasured using a pen type pHmeter to know the content ofvitamin c (ascorbic acid) in eachtype of fruit juices
Instruments/Apparatus:Pen type pH meter, four 250mlbeakers, polystyrene cup, paper towels
Materials:Different types of fruit juices ofthe same brand, buffer solution
1. Four 250ml beakers are prepared and labelledA, B, C, D.2. 200ml of the fruit juices of different flavours arepoured into the following labelled beakers.3. 100ml of buffer solution is measuredand poured into a polystyrene cup. Thecap of the pen type pH meter is removedand turned on. The probe end of the pentype pH meter is immersed into thebuffer solution.
6. The pen type pH then is immersed intothe fruit juice labelled A to obtain its pHvalue.7. The pen type pH meter is swirled in thefruit juice to prevent air bubbles and isleft in the solution until a stable reading isdisplayed.8. The value shown on the pen type pHmeter is recorded in a table.9. The steps 6 to 9 are repeated using theother sample of fruit juices in beakerB, C, and D.4. The pen type pH meter isswirled in the buffer solution toprevent air bubbles and is left inthe solution for at least 30seconds until it reads pH 7.5. The pen type pH is rinsed underrunning water and excess liquid isdried with a paper towel.
Results:3.1 126.96.36.199.13.03.23.32.852.92.9533.053.13.153.188.8.131.52A B C DReading 1 Reading 2
Food with high amount of vitamin c(ascorbic acid)Fruits Vegetables
Benefits of vitamin c (ascorbic acid) tohuman bodyGrowth And Repair Of TissuesPrevent the build-up of radicalsMaintainshealthybones andteeth
Deficiency of vitamin c(ascorbic acid)AnaemiaBleeding gumsNose bleedingScaly skinWeight gain
Conclusion• All the fruit juice A, B, C, D, had the same vitamin Ccontent due to the insignificant difference in the pH.• Form our observation of the nutritional information ofthe fruit juices on the back of the box, our conclusionwas also strengthened. We saw that all the vitamin Ccontents in the fruit juice were the same (39mg) forjuice A, B, C and D.• Different types of fruit juice of the same brand docontain the same content of vitamin C. The vitamin Ccontent is not affected by the type of fruit juice if thefruit juice came from the same brand.
APPRECIATIONWe would like to express our heartfelt gratitude especially to:1. Pn Betty Wong, our beloved teacher advisor for giving us a lot of guidanceand advices. Not only that, we will not forget the help she gave us insolving the problems we encountered throughout the research.2. The school computer lab, for following us to access the Internet withease in order to obtain the information that we were looking for. Besidesthat, we would also like to thank the school library for providing us witha wide range of reading materials.3. All parties that also succeeded this research.