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MAPPING OF WHISKER SENSORY INPUT IN THE TRIGEMINAL GANGLION OF THE MOUSE Stephanie Redmond David W. Matthews Dr. David Kleinfeld University of California, San Diego
Mapping the Trigeminal Ganglion Where is the trigeminal ganglion (TG)? What makes up the TG? Whisker follicle injections Image analysis TG patterning Going forward Adapted from Kleinfeld, et al. 2008
Where is the TG? Adapted from Kleinfeld, et al. 2006
What makes up the TG? Many different cell types Whisker sensory neurons Temperature-sensing neurons Motor (movement) neurons Functions Correlations to vision Hyperacuity Trigeminal neuralgia Blood vessels rub against TG cells M R C L rostral(R), caudal (C), lateral (L), medial (M)
Why should spatial organization exist? TG Studies: Anatomy Gene Expression Adapted from Waite 1992 Adapted from Hodge 2007
Whisker Follicle Injections Fluorescent retrograde neuron labeling Alexa Fluor® Cholera Toxin Subunit B Wild type mice, age ~P16 TG histologicalslice 50 to 75 micrometer slices 488 nm 555 nm 647 nm