MAPPING OF WHISKER SENSORY INPUT IN
THE TRIGEMINAL GANGLION OF THE
MOUSE
Stephanie Redmond
David W. Matthews
Dr. David Kle...
Mapping the Trigeminal Ganglion
• Where is the trigeminal ganglion (TG)?
• What makes up the TG?
• Whisker follicle inject...
Where is the TG?
Adapted from Kleinfeld, et al. 2006
What makes up the TG?
• Many different cell types
– Whisker sensory neurons
– Temperature-sensing neurons
– Motor (movemen...
Why should spatial organization
exist?
TG Studies:
• Anatomy
• Gene Expression
Adapted from Waite 1992 Adapted from Hodge ...
Whisker Follicle Injections
• Fluorescent retrograde neuron labeling
– Alexa Fluor® Cholera Toxin Subunit B
– Wild type mi...
Image Analysis
• Confocal Microscopy
• Image through sample (~50-100 um)
• Develop image stacks
"Confocal Microscopy", D S...
Sample E
A (488) B (555) C (647)
R
M
L
C
Image Analysis
• Spatial Organization
• Measure cell height, width, length
• Calculate and compare average:
• Position, vo...
TG Patterning
0
200
400
600
800
1000
1200
A B C D E
SquareMicrometers
Whisker
Average Cell Cross-sectional Area
1
2
3
4
5
Going Forward
• Process image data
• Investigate other patterns
• Average X-coordinate
• Average Y-coordinate
• Average vo...
Acknowledgments
David W. Matthews
Dr. David Kleinfeld
The Kleinfeld Lab
Physics REU Program
UCSD
MPS Faculty
Professor Shi...
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Mapping of Vibrissa Sensory Input in the Trigeminal - MAPPING OF ...

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  • Bigger Picture!
  • Animals with Whiskers – 2 types
  • What did I actually do? (follicle injections)
  • Now that we have the labeled tissue, used Confocal microscopy to get…
  • Mapping of Vibrissa Sensory Input in the Trigeminal - MAPPING OF ...

    1. 1. MAPPING OF WHISKER SENSORY INPUT IN THE TRIGEMINAL GANGLION OF THE MOUSE Stephanie Redmond David W. Matthews Dr. David Kleinfeld University of California, San Diego
    2. 2. Mapping the Trigeminal Ganglion • Where is the trigeminal ganglion (TG)? • What makes up the TG? • Whisker follicle injections • Image analysis • TG patterning • Going forward Adapted from Kleinfeld, et al. 2008
    3. 3. Where is the TG? Adapted from Kleinfeld, et al. 2006
    4. 4. What makes up the TG? • Many different cell types – Whisker sensory neurons – Temperature-sensing neurons – Motor (movement) neurons • Functions – Correlations to vision • Hyperacuity – Trigeminal neuralgia • Blood vessels rub against TG cells R M L C rostral (R), caudal (C), lateral (L), medial (M)
    5. 5. Why should spatial organization exist? TG Studies: • Anatomy • Gene Expression Adapted from Waite 1992 Adapted from Hodge 2007
    6. 6. Whisker Follicle Injections • Fluorescent retrograde neuron labeling – Alexa Fluor® Cholera Toxin Subunit B – Wild type mice, age ~P16 • TG histological slice – 50 to 75 micrometer slices 488 nm 555 nm 647 nm
    7. 7. Image Analysis • Confocal Microscopy • Image through sample (~50-100 um) • Develop image stacks "Confocal Microscopy", D Semwogerere & ER Weeks, published in the Encyclopedia of Biomaterials and Biomedical Engineering, Taylor & Francis (2005). Screen with pinhole Focal Point
    8. 8. Sample E A (488) B (555) C (647) R M L C
    9. 9. Image Analysis • Spatial Organization • Measure cell height, width, length • Calculate and compare average: • Position, volume A (488) B (555) C (647) R M L C
    10. 10. TG Patterning 0 200 400 600 800 1000 1200 A B C D E SquareMicrometers Whisker Average Cell Cross-sectional Area 1 2 3 4 5
    11. 11. Going Forward • Process image data • Investigate other patterns • Average X-coordinate • Average Y-coordinate • Average volume • Finish thesis paper • Graduate
    12. 12. Acknowledgments David W. Matthews Dr. David Kleinfeld The Kleinfeld Lab Physics REU Program UCSD MPS Faculty Professor Shilepsky Professor Heinekamp MPS Majors Wells College
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