Spotfire_8

Spotﬁre 8

Spotﬁre

• • DBCLS • DNA twitter id : yag_ays http://g86.dbcls.jp/~yag/wordpress/

• Spotﬁre • Spotﬁre 3.0 • • 7 9

• • • de novo RNA-Seq SNP ChIP-Seq

read length day / run base/run (base) (Gb) Solexa (50bp) 75 9.5 20.5 - 25 SOLiD3 50 6-7 20 - 30 454 FLX Ti (330bp) 400 0.4 0.4 - 0.6 http://spreadsheets.google.com/pub?key=tQ5PDSBsSTkLXhDQFugandg&output=html

• • • ( ) http://g86.dbcls.jp/mtblm/b/NM_133977

Cell AAAAA mRNA AAAAA AAAAA RNA fragments Mapping

ORF gt ag ag or

• • • FPKM (Fragments Per Kilobase of exon model per Million mapped fragments) ID

Refseq id diff FPKM...etc

Reference Analyzed data diff

Spotﬁre 1 2 3

Spotﬁre •

http://g86.dbcls.jp/mtblm/

• Spotﬁre http://g86.dbcls.jp/~yag/spotﬁre.pdf

Spotfire_8

by yag ays on Nov 04, 2010

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