FBW                   21-02-2008RELOADED                         2           Wim Van Criekinge
Three Basic Data Types                  • Scalars - $                  • Arrays of scalars - @                  • Associat...
• [m]/PATTERN/[g][i][o]• s/PATTERN/PATTERN/[g][i][e][o]• tr/PATTERNLIST/PATTERNLIST/[c][d][s]
The ‘structure’ of a Hash • An array looks something like this:                      0       1     2      Index      @arra...
Sub routine       $a=5;       $b=9;       $sum=Optellen(5,9);       print "The SUM is $sumn";       sub Optellen()       {...
Overview           • Advanced data structures in Perl           • Object-oriented Programming in Perl           • Bioperl:...
Multi-line parsing                     use strict;                     use Bio::SeqIO;                     my $filename="s...
Perl 00          • A class is a package          • An object is a reference to a data            structure (usually a hash...
Perl Classes               • Modules/Packages                 – A Perl module is a file that uses a package               ...
Installation on Windows (ActiveState)                       • Using PPM shell to install BioPerl                          ...
Directory Structure                  • BioPerl directory structure organization:                      – Bio/       BioPerl...
Live.pl          #!e:Perlbinperl.exe -w          # script for looping over genbank entries, printing out name          use...
File converter#!/opt/perl/bin/perl -w#genbank_to_fasta.pluse Bio::SeqIO;my $input = Bio::SeqIO::new->(‘-file’ => $ARGV[0],...
• Bptutorial.pl• It includes the written tutorial as well  as runnable scripts• 2 ESSENTIAL TOOLS  – Data::Dumper to find ...
Oefening 1Run Needleman-Wunsch-monte-carlo.pl–     my $MATCH    = 1; # +1 for letters that match–     my $MISMATCH = -1; #...
A Guide to MySQL & DBI
Objectives• Start MySQL and learn how to use the MySQL  Reference Manual• Create a database• Change (activate) a database•...
Objectives (continued)• Identify and use data types to define columns in  tables• Understand and use nulls• Add rows to ta...
Opvolger voor MySQL Front          • MySQL-Front was destijds een van            de meest populaire MySQL-            mana...
Starting MySQL• Windows XP   –   Click Start button   –   Point to All Programs   –   Point to MySQL on menu   –   Point t...
Obtaining Help in MySQL• Type h at MySQL> prompt• Type “help” followed by name of command  – help contents  – help union
Creating a Database• Must create a database before creating tables• Use CREATE DATABASE command• Include database name
Creating a Database (continued)
Changing the Default Database• Default database: database to which all  subsequent commands pertain• USE command, followed...
Creating a Table  • Describe the layout of each table in the    database  • Use CREATE TABLE command  • TABLE is followed ...
Table and Column Name Restrictions• Names cannot exceed 18 characters• Must start with a letter• Can contain letters, numb...
Creating the REP Table
Entering Commands in MySQL• Commands are free-format; no rules stating specific  words in specific positions• Press ENTER ...
Running SQL Commands
Editing SQL Commands• Statement history: stores most recently used  command• Editing commands:   –   Use arrow keys to mov...
Errors in SQL Commands
Editing MySQL Commands• Press Up arrow key to go to top line• Press Enter key to move to next line if line is correct• Use...
Dropping a Table• Can correct errors by dropping (deleting) a table and  starting over• Useful when table is created befor...
Data Types  • For each table column, type of data must be    defined  • Common data types:     – CHAR(n)     – VARCHAR(n) ...
Nulls• A special value to represent situation when actual  value is not known for a column• Can specify whether to allow n...
Implementation of Nulls• Use NOT NULL clause in CREATE TABLE  command to exclude the use of nulls in a column• Default is ...
Adding Rows to a Table• INSERT command:   – INSERT INTO followed by table name   – VALUES command followed by specific val...
The Insert Command
Modifying the INSERT Command• To add new rows modify previous INSERT command• Use same editing techniques as those used to...
Adding Additional Rows
The INSERT Command with Nulls• Use a special format of INSERT command to enter a  null value in a table• Identify the name...
The INSERT Command with Nulls• Enter only non-null values• Precisely indicate values you are entering by listing  the colu...
The INSERT Command with Nulls (continued)
Viewing Table Data• Use SELECT command to display all the rows and  columns in a table• SELECT * FROM followed by the name...
Viewing Table Data (continued)
Viewing Table Data (continued)
Correcting Errors In the Database• UPDATE command is used to update a value in a  table• DELETE command allows you to dele...
Correcting Errors in the Database• UPDATE: change the value in a table• DELETE: delete a row from a table
Correcting Errors in the Database (continued)
Correcting Errors in the Database (continued)
Saving SQL Commands• Allows you to use commands again without retyping• Different methods for each SQL implementation you ...
Saving SQL Commands• Script file:   –   File containing SQL commands   –   Use a text editor or word processor to create  ...
Creating the Remaining Database Tables• Execute appropriate CREATE TABLE and INSERT  commands• Save these commands to a se...
Describing a Table
Summary• Use MySQL Command Line Client window to enter  commands• Type h or help to obtain help at the mysql> prompt• Use ...
Summary (continued)• Use the CREATE DATABASE command to create a  database• Use the USE command to change the default  dat...
Summary (continued)• CHAR, VARCHAR, DATE, DECIMAL, INT and  SMALLINT data types• Use INSERT command to add rows• Use NOT N...
Summary (continued)• Use UPDATE command to change the value in a  column• Use DELETE command to delete a row• Use SHOW COL...
• DBI
•   use DBI;•   my $dbh = DBI->connect( dbi:mysql:guestdb,•                 root,•                 ,•               ) || d...
The Players• Perl – a programming language• DBMS – software to manage datat storage• SQL – a language to talk to a DBMS• D...
• What is DBI ?• DBI is a DataBase Interface  – It is the way Perl talks to Databases• DBI is a module by Tim Bunce• DBI i...
• What is an interface ?• The overlap where two phenomeba affect  each other• A point at which independent systems interac...
• A Sample Interface (the bedrock of DBI)                     Bone           Fred               Wilma                     ...
• Characteristics of the DINO interface• Separation of knowledge  – Fred doesn’t need to know how to find Wilma  – Dino do...
• The DBI interface                      SQL           Perl             DBMS                      DBI
• Characteristics of the DBI interface• Separation of knowledge  – You don’t need to know how to connect  – DBI doesn’t ne...
• The ingredients of a DBI App  – 1: A perl script that uses DBI  – 2: A DBMS  – 3: SQL statements
Outline of a basic DBI scriptSet the Perl EnvironmentConnect to a DBMSPerform data-affecting SQL instructionsPerform data-...
• $dbh = DataBase Handle• Done by DBI  – Connect• Done by $dbh, The Database Handle  – Perform SQL instructions  – Perform...
• Set the Perl Environment  – use warnings;  – use strict;  – Use DBI;
• Connect to a DBMSmy $dbh = DBI -> connect (‘dbi:DBM:’)$dbh is a Database Handke  An object created by DBI to handle acce...
• Perform data-affecting Instructions• $dbh->do($sql_string);• $dbh->do(“ INSERT INTO geography  VALUES (‘Nepal’,’Asia’)” );
• Perform data-returning requests• My @row = $dbh-  >selectrow_array($sql_string)• Disconnect from DBMS• $dbh->disconnect()
A complete script• use strict;• use warnings;• use DBI;• my $dbh=DBI->connect("dbi:mysql:test","root","");• $dbh->do("CREA...
• The script output• Only one row• No seperation of the fields• No metadata
• Improvements• DBI  – Connect to DBMS  – Creates a database handle ($dbh)• $dbh  – Provides course-grained access to the ...
• Life-cycle of a statement handle ($sth)• Prepare  – Creates the handle, sends SQL to the DBMS to    be analyzed and opti...
• Life-cycle of a statement handle ($sth)• My $sth = $dbh->prepare($sql_string);• $sth->execute();• Print $sth->fetchrow_a...
• Fecthing rows in a loop – the snippet• My $sth=$dbh->prepare(“SELECT * FROM  geography”);• $sth->execute();• While (my @...
• Output    – Nepal:Asia    – Portugal:Europe•   All data retrieved•   Colums seperated•   Rows seperated•   Still no meta...
• Finding Metadata – Handle Attributes• $handle->{$key}=$value;• Print $handle->{$key};• $dbh->{RaiseError}=1;• Print $dbh...
• Finding Metadata with $sth->{NAME}• my $sth=$dbh->prepare(“SELECT * FROM  geography”);• $sth->execute();• my @column_nam...
• Errors• $dbh->do (“Junk”);• Print “I Got here!”;
• Checking Errors with RaiseError• my $dbh=DBI->connect >..• $dbh->{RaiseError}=1;• $dbh->do(“Junk”);• Print “Here ?”;
Number of rows affected$rows=$dbh->do(“DELETE FROM user  WHERE age <42”);# undef = error# 3 = 3 rows affected# 0E0 = no er...
• Summary so far• DBI connect($data_source)•   $dbh do($sql_instruction)•   Prepare ($sql_request)•   Disconnect()•   {Rai...
• A Deeper look at connection                           DBD#1   MySQL          Perl       DBI                             ...
• DBDs- Database Drivers• DRIVER          DBMS• DBD::DBM        DBM• DBD::Pg         postgreSQL• DBD::mysql      MySQL• DB...
• Variation in DBDs & DBMSs•   Driver-specific connection parameters•   Driver-specific attributes and methods•   SQL impl...
• Driver-Specific Connection Params – driver name – user  name and password•   My $dbh = DBI->connect(•   “DBI:$driver:”,•...
Finish() – fetchus interuptusWhile (my @row=$sth->fetchrow_array){Last if $row[0] eq $some_conditions;}$sth->finish();
• Alternate fecthes• My @row=$sth->fetchrow_array();  – Print $row[1];• My @row=$sth->fetchrow_arrayref();  – Print $row->...
• Placeholders !• my $sth = $dbh -> prepare (“SELECT name  from user WHERE country = ? AND city = ?  AND age > ?”);• $sth-...
• DBDs that don’t need a separate DBMS• DBD::CSV, DBD::Excel• DBD::Amazon DBD::Google• use DBI; my $dbh = DBI-  >connect("...
Step1: Getting DriversEssential for SQL Querying• A driver is a piece of software that lets your  operating system talk to...
MySQL Driver: Needed to Query MySQL Databases  • Windows: Download MySQL    Connector/ODBC 3.51 here  • Must be installed ...
Oefening 2Fetch a sequence by adapting live.pl and do remote blast using 3different scoring matrices (summarize results) a...
Parsing BLAST Using BPlite, BPpsilite, and BPbl2seq                   • Similar to Search and SearchIO in                 ...
Parse BLAST output#!/opt/perl/bin/perl -w#bioperl_blast_parse.pl# program prints out query, and all hits with scores for e...
Parse BLAST in a little more detail#!/opt/perl/bin/perl -w#bioperl_blast_parse_hsp.pl# program prints out query, and all h...
Shuffle   #!/usr/bin/perl -w   use strict;   my ($def, @seq) = <>;   print $def;   chomp @seq;   @seq = split(//, join("",...
Searching for Sequence Similarity• BLAST with BioPerl• Parsing Blast and FASTA Reports   – Search and SearchIO   – BPLite,...
Remote Execution of BLAST• BioPerl has built in capability of running BLAST jobs remotely  using RemoteBlast.pm• Runs thes...
Example of Remote BlastA script to run a remote blast would be something like the following skeleton:$remote_blast = Bio::...
ExampleIt is important to note that all command line options that fall under the blastall     umbrella are available under...
Parsing BLAST and FASTA Reports• Main BioPerl objects in 1.2 are  Search.pm/SearchIO.pm   – SearchIO is more robust and th...
Parsing Blast Reports• One of the strengths of BioPerl is its ability to  parse complex data structures. Like a blast  rep...
Sample Script to Read and Parse BLAST Report# Get the report $searchio = new Bio::SearchIO (-format => blast, -file =>   $...
2012 03 08_dbi
2012 03 08_dbi
2012 03 08_dbi
2012 03 08_dbi
2012 03 08_dbi
2012 03 08_dbi
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2012 03 08_dbi

  1. 1. FBW 21-02-2008RELOADED 2 Wim Van Criekinge
  2. 2. Three Basic Data Types • Scalars - $ • Arrays of scalars - @ • Associative arrays of scalers or Hashes - %
  3. 3. • [m]/PATTERN/[g][i][o]• s/PATTERN/PATTERN/[g][i][e][o]• tr/PATTERNLIST/PATTERNLIST/[c][d][s]
  4. 4. The ‘structure’ of a Hash • An array looks something like this: 0 1 2 Index @array = val1 val2 val3 Value • A hash looks something like this: Rob Matt Joe_A Key (name)%phone = 353-7236 353-7122 555-1212 Value
  5. 5. Sub routine $a=5; $b=9; $sum=Optellen(5,9); print "The SUM is $sumn"; sub Optellen() { $d=@_[0]; $e=@_[1]; #alternatively we could do this: my($a, $b)=@_; my($answer)=$d+$e; return $answer; }
  6. 6. Overview • Advanced data structures in Perl • Object-oriented Programming in Perl • Bioperl: is a large collection of Perl software for bioinformatics • Motivation: – Simple extension: “Multiline parsing“ more difficult than expected • Goal: to make software modular, easier to maintain, more reliable, and easier to reuse
  7. 7. Multi-line parsing use strict; use Bio::SeqIO; my $filename="sw.txt"; my $sequence_object; my $seqio = Bio::SeqIO -> new ( -format => swiss, -file => $filename ); while ($sequence_object = $seqio -> next_seq) { my $sequentie = $sequence_object-> seq(); print $sequentie."n"; }
  8. 8. Perl 00 • A class is a package • An object is a reference to a data structure (usually a hash) in a class • A method is a subroutine in the class
  9. 9. Perl Classes • Modules/Packages – A Perl module is a file that uses a package declaration – Packages provide a separate namespace for different parts of program – A namespace protects the variable of one part of a program from unwanted modification by another part of the program – The module must always have a last line that evaluates to true, e.g. 1; – The module must be in “known” directory (environment variable) • Eg … site/lib/bio/Sequentie.pm
  10. 10. Installation on Windows (ActiveState) • Using PPM shell to install BioPerl – Get the number of the BioPerl repository: – PPM>repository – Set the BioPerl repository, find BioPerl, install BioPerl: – PPM>repository set <BioPerl repository number> – PPM>search * – PPM>install <BioPerl package number> • Download BioPerl in archive form from – http://www.BioPerl.org/Core/Latest/index.shtml – Use winzip to uncompress and install
  11. 11. Directory Structure • BioPerl directory structure organization: – Bio/ BioPerl modules – models/ UML for BioPerl classes – t/ Perl built-in tests – t/data/ Data files used for the tests – scripts/ Reusable scripts that use BioPerl – scripts/contributed/ Contributed scripts not necessarily integrated into BioPerl. – doc/ "How To" files and the FAQ as XML
  12. 12. Live.pl #!e:Perlbinperl.exe -w # script for looping over genbank entries, printing out name use Bio::DB::Genbank; use Data::Dumper; $gb = new Bio::DB::GenBank(); $sequence_object = $gb->get_Seq_by_id(MUSIGHBA1); print Dumper ($sequence_object); $seq1_id = $sequence_object->display_id(); $seq1_s = $sequence_object->seq(); print "seq1 display id is $seq1_id n"; print "seq1 sequence is $seq1_s n";
  13. 13. File converter#!/opt/perl/bin/perl -w#genbank_to_fasta.pluse Bio::SeqIO;my $input = Bio::SeqIO::new->(‘-file’ => $ARGV[0], ‘-format’ => ‘GenBank’);my $output = Bio::SeqIO::new->(‘-file’ => ‘>output.fasta’, ‘-format’ => ‘Fasta’);while (my $seq = $input->next_seq()){ $output->write_seq($seq)}
  14. 14. • Bptutorial.pl• It includes the written tutorial as well as runnable scripts• 2 ESSENTIAL TOOLS – Data::Dumper to find out what class your in – Perl bptutorial (100 Bio::Seq) to find the available methods for that class
  15. 15. Oefening 1Run Needleman-Wunsch-monte-carlo.pl– my $MATCH = 1; # +1 for letters that match– my $MISMATCH = -1; # -1 for letters that mismatch– my $GAP = -1; # -1 for any gap Score (-64) Score = f($MATCH,$MISMATCH,$GAP)f?Implement convergence criteriaStore in DATABASE, make graphs in Excel
  16. 16. A Guide to MySQL & DBI
  17. 17. Objectives• Start MySQL and learn how to use the MySQL Reference Manual• Create a database• Change (activate) a database• Create tables using MySQL• Create and run SQL commands in MySQL
  18. 18. Objectives (continued)• Identify and use data types to define columns in tables• Understand and use nulls• Add rows to tables• View table data• Correct errors in a database
  19. 19. Opvolger voor MySQL Front • MySQL-Front was destijds een van de meest populaire MySQL- management applicaties. Wat PHPMyAdmin voor webapplicaties is, was MySQL-Front dat voor de desktop. Helaas kon /wilde de auteur niet langer doorgaan met het project en werd het project stilgelegd. • In begin April 2006 heeft de originele auteur besloten om de laatste broncode voor MySQL-Front beschikbaar te maken onder de naam HeidiSQL en de eerste beta is
  20. 20. Starting MySQL• Windows XP – Click Start button – Point to All Programs – Point to MySQL on menu – Point to MySQL Server 4.1 – Click MySQL Command Line Client• Must enter password in Command Line Client window
  21. 21. Obtaining Help in MySQL• Type h at MySQL> prompt• Type “help” followed by name of command – help contents – help union
  22. 22. Creating a Database• Must create a database before creating tables• Use CREATE DATABASE command• Include database name
  23. 23. Creating a Database (continued)
  24. 24. Changing the Default Database• Default database: database to which all subsequent commands pertain• USE command, followed by database name: – Changes the default database – Execute at the start of every session
  25. 25. Creating a Table • Describe the layout of each table in the database • Use CREATE TABLE command • TABLE is followed by the table name • Follow this with the names and data types of the columns in the table • Data types define type and size of data
  26. 26. Table and Column Name Restrictions• Names cannot exceed 18 characters• Must start with a letter• Can contain letters, numbers, and underscores (_)• Cannot contain spaces
  27. 27. Creating the REP Table
  28. 28. Entering Commands in MySQL• Commands are free-format; no rules stating specific words in specific positions• Press ENTER to move to the next line in a command• Indicate the end of a command by typing a semicolon• Commands are not case sensitive
  29. 29. Running SQL Commands
  30. 30. Editing SQL Commands• Statement history: stores most recently used command• Editing commands: – Use arrow keys to move up, down, left, and right – Use Ctrl+A to move to beginning of line – Use Ctrl+E to move to end of line – Use Backspace and Delete keys
  31. 31. Errors in SQL Commands
  32. 32. Editing MySQL Commands• Press Up arrow key to go to top line• Press Enter key to move to next line if line is correct• Use Right and Left arrow keys to move to location of error• Press ENTER key when line is correct• If Enter is not pressed on a line, line not part of the revised command
  33. 33. Dropping a Table• Can correct errors by dropping (deleting) a table and starting over• Useful when table is created before errors are discovered• Command is followed by the table to be dropped and a semicolon• Any data in table also deleted
  34. 34. Data Types • For each table column, type of data must be defined • Common data types: – CHAR(n) – VARCHAR(n) – DATE – DECIMAL(p,q) – INT – SMALLINT
  35. 35. Nulls• A special value to represent situation when actual value is not known for a column• Can specify whether to allow nulls in the individual columns• Should not allow nulls for primary key columns
  36. 36. Implementation of Nulls• Use NOT NULL clause in CREATE TABLE command to exclude the use of nulls in a column• Default is to allow null values• If a column is defined as NOT NULL, system will reject any attempt to store a null value there
  37. 37. Adding Rows to a Table• INSERT command: – INSERT INTO followed by table name – VALUES command followed by specific values in parentheses – Values for character columns in single quotation marks
  38. 38. The Insert Command
  39. 39. Modifying the INSERT Command• To add new rows modify previous INSERT command• Use same editing techniques as those used to correct errors
  40. 40. Adding Additional Rows
  41. 41. The INSERT Command with Nulls• Use a special format of INSERT command to enter a null value in a table• Identify the names of the columns that accept non- null values, then list only the non-null values after the VALUES command
  42. 42. The INSERT Command with Nulls• Enter only non-null values• Precisely indicate values you are entering by listing the columns
  43. 43. The INSERT Command with Nulls (continued)
  44. 44. Viewing Table Data• Use SELECT command to display all the rows and columns in a table• SELECT * FROM followed by the name of the table• Ends with a semicolon
  45. 45. Viewing Table Data (continued)
  46. 46. Viewing Table Data (continued)
  47. 47. Correcting Errors In the Database• UPDATE command is used to update a value in a table• DELETE command allows you to delete a record• INSERT command allows you to add a record
  48. 48. Correcting Errors in the Database• UPDATE: change the value in a table• DELETE: delete a row from a table
  49. 49. Correcting Errors in the Database (continued)
  50. 50. Correcting Errors in the Database (continued)
  51. 51. Saving SQL Commands• Allows you to use commands again without retyping• Different methods for each SQL implementation you are using – Oracle SQL*Plus and SQL*Plus Worksheet use a script file – Access saves queries as objects – MySQL uses an editor to save text files
  52. 52. Saving SQL Commands• Script file: – File containing SQL commands – Use a text editor or word processor to create – Save with a .txt file name extension – Run in MySQL: • SOURCE file name • . file name – Include full path if file is in folder other than default
  53. 53. Creating the Remaining Database Tables• Execute appropriate CREATE TABLE and INSERT commands• Save these commands to a secondary storage device
  54. 54. Describing a Table
  55. 55. Summary• Use MySQL Command Line Client window to enter commands• Type h or help to obtain help at the mysql> prompt• Use MySQL Reference Manual for more detailed help
  56. 56. Summary (continued)• Use the CREATE DATABASE command to create a database• Use the USE command to change the default database• Use the CREATE TABLE command to create tables• Use the DROP TABLE command to delete a table
  57. 57. Summary (continued)• CHAR, VARCHAR, DATE, DECIMAL, INT and SMALLINT data types• Use INSERT command to add rows• Use NOT Null clause to identify columns that cannot have a null value• Use SELECT command to view data in a table
  58. 58. Summary (continued)• Use UPDATE command to change the value in a column• Use DELETE command to delete a row• Use SHOW COLUMNS command to display a table’s structure
  59. 59. • DBI
  60. 60. • use DBI;• my $dbh = DBI->connect( dbi:mysql:guestdb,• root,• ,• ) || die "Database connection not made: $DBI::errstr";• $sth = $dbh->prepare(SELECT * FROM demo);• $sth->execute();• while (my @row = $sth->fetchrow_array) {• print join(":",@row),"n";• }• $sth->finish();• $dbh->disconnect();
  61. 61. The Players• Perl – a programming language• DBMS – software to manage datat storage• SQL – a language to talk to a DBMS• DBI – Perl extensions to send SQL to a DBMS• DBD – software DBI uses for specific DBMSs• $dbh – a DBI object for course-grained access• $sth – a DBI object for fine-grained access
  62. 62. • What is DBI ?• DBI is a DataBase Interface – It is the way Perl talks to Databases• DBI is a module by Tim Bunce• DBI is a community of modules & developers
  63. 63. • What is an interface ?• The overlap where two phenomeba affect each other• A point at which independent systems interact• A boundary across which two systems communicate
  64. 64. • A Sample Interface (the bedrock of DBI) Bone Fred Wilma Dino
  65. 65. • Characteristics of the DINO interface• Separation of knowledge – Fred doesn’t need to know how to find Wilma – Dino doesn’t need to know how to read• Generalizability – Fred can send any message – Fred can communicate with anyone
  66. 66. • The DBI interface SQL Perl DBMS DBI
  67. 67. • Characteristics of the DBI interface• Separation of knowledge – You don’t need to know how to connect – DBI doesn’t need to know SQL• Generalizeability – You can send any SQL – You can communicate with any DBMS
  68. 68. • The ingredients of a DBI App – 1: A perl script that uses DBI – 2: A DBMS – 3: SQL statements
  69. 69. Outline of a basic DBI scriptSet the Perl EnvironmentConnect to a DBMSPerform data-affecting SQL instructionsPerform data-returning SQL requestsDisconnect from the DBMS
  70. 70. • $dbh = DataBase Handle• Done by DBI – Connect• Done by $dbh, The Database Handle – Perform SQL instructions – Perform SQL request – Disconnect
  71. 71. • Set the Perl Environment – use warnings; – use strict; – Use DBI;
  72. 72. • Connect to a DBMSmy $dbh = DBI -> connect (‘dbi:DBM:’)$dbh is a Database Handke An object created by DBI to handle access to this specific connection
  73. 73. • Perform data-affecting Instructions• $dbh->do($sql_string);• $dbh->do(“ INSERT INTO geography VALUES (‘Nepal’,’Asia’)” );
  74. 74. • Perform data-returning requests• My @row = $dbh- >selectrow_array($sql_string)• Disconnect from DBMS• $dbh->disconnect()
  75. 75. A complete script• use strict;• use warnings;• use DBI;• my $dbh=DBI->connect("dbi:mysql:test","root","");• $dbh->do("CREATE TABLE geography (country Text, region Text)");• $dbh->do("INSERT INTO geography VALUES (Nepal,Asia)");• $dbh->do("INSERT INTO geography VALUES (Portugal,Europe)");• print $dbh->selectrow_array("SELECT * FROM geography");• $dbh->disconnect
  76. 76. • The script output• Only one row• No seperation of the fields• No metadata
  77. 77. • Improvements• DBI – Connect to DBMS – Creates a database handle ($dbh)• $dbh – Provides course-grained access to the DBMS – Creates a statement handle ($sth)• $sth – Provides fine-grained access to the DBMS
  78. 78. • Life-cycle of a statement handle ($sth)• Prepare – Creates the handle, sends SQL to the DBMS to be analyzed and optimized• Execute – Instructs the DBMS to perform operations• Fetch – Brings data from the DBMS into a script
  79. 79. • Life-cycle of a statement handle ($sth)• My $sth = $dbh->prepare($sql_string);• $sth->execute();• Print $sth->fetchrow_array();
  80. 80. • Fecthing rows in a loop – the snippet• My $sth=$dbh->prepare(“SELECT * FROM geography”);• $sth->execute();• While (my @row = $sth->fetchrow_array){• Print join(“:”,@row),”n”;• }
  81. 81. • Output – Nepal:Asia – Portugal:Europe• All data retrieved• Colums seperated• Rows seperated• Still no metadata
  82. 82. • Finding Metadata – Handle Attributes• $handle->{$key}=$value;• Print $handle->{$key};• $dbh->{RaiseError}=1;• Print $dbh->{RaiseError};• My $column_names = $sth->{NAME};
  83. 83. • Finding Metadata with $sth->{NAME}• my $sth=$dbh->prepare(“SELECT * FROM geography”);• $sth->execute();• my @column_names=@{$sth->{NAME}};• my $num_cols = scaler @column_names;• print join “:”,@column_names;• print “(there are $num_cols columns)”;
  84. 84. • Errors• $dbh->do (“Junk”);• Print “I Got here!”;
  85. 85. • Checking Errors with RaiseError• my $dbh=DBI->connect >..• $dbh->{RaiseError}=1;• $dbh->do(“Junk”);• Print “Here ?”;
  86. 86. Number of rows affected$rows=$dbh->do(“DELETE FROM user WHERE age <42”);# undef = error# 3 = 3 rows affected# 0E0 = no error; no rows affected# -1 = unknown
  87. 87. • Summary so far• DBI connect($data_source)• $dbh do($sql_instruction)• Prepare ($sql_request)• Disconnect()• {RaiseError}• $sth execute() – Fetchrow_array() – {NAMEM}
  88. 88. • A Deeper look at connection DBD#1 MySQL Perl DBI Oracle DBD#2
  89. 89. • DBDs- Database Drivers• DRIVER DBMS• DBD::DBM DBM• DBD::Pg postgreSQL• DBD::mysql MySQL• DBD::Oracle Oracle• DBD::ODBC Ms-Access, MS-SQL- Server• …
  90. 90. • Variation in DBDs & DBMSs• Driver-specific connection parameters• Driver-specific attributes and methods• SQL implementaion• Optimization Plans
  91. 91. • Driver-Specific Connection Params – driver name – user name and password• My $dbh = DBI->connect(• “DBI:$driver:”,• “root”,• “password”;• {• RaiseError => 1,• PrinError => 0,• AutoCommit =>1,• }• );
  92. 92. Finish() – fetchus interuptusWhile (my @row=$sth->fetchrow_array){Last if $row[0] eq $some_conditions;}$sth->finish();
  93. 93. • Alternate fecthes• My @row=$sth->fetchrow_array(); – Print $row[1];• My @row=$sth->fetchrow_arrayref(); – Print $row->[1]• My @row=$sth->fetchrow_hashref(); – Print $row->{region};
  94. 94. • Placeholders !• my $sth = $dbh -> prepare (“SELECT name from user WHERE country = ? AND city = ? AND age > ?”);• $sth-> execute(‘Venezuela’,’Caracas’,21);
  95. 95. • DBDs that don’t need a separate DBMS• DBD::CSV, DBD::Excel• DBD::Amazon DBD::Google• use DBI; my $dbh = DBI- >connect("dbi:Google:", $KEY); my $sth = $dbh->prepare(qq[ SELECT title, URL FROM google WHERE q = "perl" ]); while (my $r = $sth->fetchrow_hashref) { ...
  96. 96. Step1: Getting DriversEssential for SQL Querying• A driver is a piece of software that lets your operating system talk to a database – Installed drivers visible in ODBC manager • “data connectivity” tool• Each database engine (Oracle, MySQL, etc) requires its own driver – Generally must be installed by user• Drivers are needed by Data Source Name tool and querying programs• Require (simple) installation
  97. 97. MySQL Driver: Needed to Query MySQL Databases • Windows: Download MySQL Connector/ODBC 3.51 here • Must be installed for direct querying using e.g. Excel – Not necessary if you are using the MySQL Query Browser
  98. 98. Oefening 2Fetch a sequence by adapting live.pl and do remote blast using 3different scoring matrices (summarize results) and perform“controls” using adaptation of shuffle … Rat versus Rat versus mouse RBP bacterial lipocalin
  99. 99. Parsing BLAST Using BPlite, BPpsilite, and BPbl2seq • Similar to Search and SearchIO in basic functionality • However: – Older and will likely be phased out in the near future – Substantially limited advanced functionality compared to Search and SearchIO – Important to know about because many legacy scripts utilize these objects and either need to be converted
  100. 100. Parse BLAST output#!/opt/perl/bin/perl -w#bioperl_blast_parse.pl# program prints out query, and all hits with scores for each blast resultuse Bio::SearchIO;my $record = Bio::SearchIO->new(-format => ‘blast’, -file => $ARGV[0]);while (my $result = $record->next_result){ print “>”, $result->query_name, “ “, $result->query_description, “n”; my $seen = 0; while (my $hit = $result->next_hit){ print “t”, $hit->name, “t”, $hit->bits, “t”, $hit->significance, “n”; $seen++ } if ($seen == 0 ) { print “No Hits Foundn” }}
  101. 101. Parse BLAST in a little more detail#!/opt/perl/bin/perl -w#bioperl_blast_parse_hsp.pl# program prints out query, and all hsps with scores for each blast resultuse Bio::SearchIO;my $record = Bio::SearchIO->new(-format => ‘blast’, -file => $ARGV[0]);while (my $result = $record->next_result){ print “>”, $result->query_name, “ “, $result->query_description, “n”; my $seen = 0; while (my $hit = $result->next_hit{ $seen++; while (my $hsp = $hit->next_hsp){ print “t”, $hit->name, “has an HSP with an evalue of: “, $hsp->evalue, “n”;} if ($seen == 0 ) { print “No Hits Foundn” }}
  102. 102. Shuffle #!/usr/bin/perl -w use strict; my ($def, @seq) = <>; print $def; chomp @seq; @seq = split(//, join("", @seq)); my $count = 0; while (@seq) { my $index = rand(@seq); my $base = splice(@seq, $index, 1); print $base; print "n" if ++$count % 60 == 0; } print "n" unless $count %60 == 0;
  103. 103. Searching for Sequence Similarity• BLAST with BioPerl• Parsing Blast and FASTA Reports – Search and SearchIO – BPLite, BPpsilite, BPbl2seq• Parsing HMM Reports• Standalone BioPerl BLAST
  104. 104. Remote Execution of BLAST• BioPerl has built in capability of running BLAST jobs remotely using RemoteBlast.pm• Runs these jobs at NCBI automatically – NCBI has dynamic configurations (server side) to “always” be up and ready – Automatically updated for new BioPerl Releases• Convenient for independent researchers who do not have access to huge computing resources• Quick submission of Blast jobs without tying up local resources (especially if working from standalone workstation)• Legal Restrictions!!!
  105. 105. Example of Remote BlastA script to run a remote blast would be something like the following skeleton:$remote_blast = Bio::Tools::Run::RemoteBlast->new( -prog => blastp,-data => ecoli,-expect => 1e-10 );$r = $remote_blast->submit_blast("t/data/ecolitst.fa");while (@rids = $remote_blast->each_rid ) { foreach $rid ( @rids ) {$rc = $remote_blast->retrieve_blast($rid);}}In this example we are running a blastp (pairwise comparison) using the ecoli database and a e-value threshold of 1e-10. The sequences that are being compared are located in the file “t/data/ecolist.fa”.
  106. 106. ExampleIt is important to note that all command line options that fall under the blastall umbrella are available under BlastRemote.pm.For example you can change some parameters of the remote job.Consider the following example:$Bio::Tools::Run::RemoteBlast::HEADER{MATRIX_NAME} = BLOSUM25;This basically allows you to change the matrix used to BLOSUM 25, rather than the default of BLOSUM 62.
  107. 107. Parsing BLAST and FASTA Reports• Main BioPerl objects in 1.2 are Search.pm/SearchIO.pm – SearchIO is more robust and the preferred choice (will be continued to be supported in future releases)• Support parsing of BLAST XML reports and other• Also allow the ability to parse HMMER reports• Will continue to grow and provide functionality for parsing all types of reports. This way multiple report types can be handled by simply creating multiple instantiations of the SearchIO object.
  108. 108. Parsing Blast Reports• One of the strengths of BioPerl is its ability to parse complex data structures. Like a blast report.• Unfortunately, there is a bit of arcane terminology.• Also, you have to ‘think like bioperl’, in order to figure out the syntax.• This next script might get you started
  109. 109. Sample Script to Read and Parse BLAST Report# Get the report $searchio = new Bio::SearchIO (-format => blast, -file => $blast_report);$result = $searchio->next_result; # Get info about the entire report $result- >database_name;$algorithm_type = $result->algorithm;# get info about the first hit $hit = $result->next_hit;$hit_name = $hit->name ;# get info about the first hsp of the first hit $hsp =$hit->next_hsp;$hsp_start = $hsp->query->start;

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