DPA 4100 System Micro-Flow Imaging™ or MFI is Brightwell Technologies ’ flow microscopy technology MFI is designed to precisely control the volume represented by each image frame, and therefore permits accurate measurement of very low particle concentrations in addition to particle size and morphology. The DPA4100 is Brightwell ’ s R&D grade MFI system. Brightwell DPA 4100 System (pump and sample vessel not shown)
Stability of formulation measured in terms of particle count as a function of time
Sensitive measurement of differences allowing formulation optimization
Quantification of Protein Particles in Parenteral Solutions using Light Obscuration and MFI_July'07_ImmunoGen Inc.
Image Comparison Time (hr) 0 2 4 6 24 24 hr, 0 rpm PBS Buffer Formulation Improved Formulation Quantification of Protein Particles in Parenteral Solutions using Light Obscuration and MFI_July'07_ImmunoGen Inc.
Conclusions Flow microscopy combines the speed and convenience of obscuration counters with the visual insights offered by manual microscopic analysis. The higher sensitivity and material independence of flow microscopy improves particle detection and measurement of translucent, heterogeneous, and irregularly shaped particles. Morphology-based software filters provide rapid, accurate method for isolating and classifying particle sub-populations Breadth of measurement parameters and analysis features make the technology applicable to a large number of applications ranging from contamination detection to suspension characterization.