Column Chromatography and SDS-Page       In this workshop we learned about protein isolation and electrophoreticcharacteri...
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Column chromatography and sds4

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Column chromatography and sds4

  1. 1. Column Chromatography and SDS-Page In this workshop we learned about protein isolation and electrophoreticcharacterization. Proteins are primary structures that serve as support, catalysts, andtransporters. All cells consist of thousands of different proteins. Protein isolation is veryimportant for industrial use and research (i.e. the study of cancer, HIV, and other diseases.).One of the many techniques used for protein isolation-and the one we used for the workshop-is ion-exchange chromatography. This consists of the binding and separation of proteins basedon charge interactions. For this workshop we had to isolate the proteins found in egg whiteusing CM-Sepharose chromatography (a type of ion-exchange chromatography). To determineif our protein was properly purified we ran the proteins on an SDS PAGE, which is a gel-basedtechnique that separates proteins according to their size. Once we ran our gel we stained itwith coomassie blue and observed our results. We were successful in isolating our proteins,since our gel showed bands.

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