As the development of mobile technology keeps marching forward, a noticeable trend of integrating location- and orientation-aware sensors (i.e., GPS receiver, digital compass, and accelerometer) into today’s smartphones has significantly promoted the development of mobile Location Based Services (LBS). At the same time, geospatial information is increasingly recognized as the common denominator in both today’s “Web 2.0” peer-to-peer social network era and tomorrow’s “Web 4.0”. As a result, embracing the growing rich spatial resources and adopting them for the mobile geospatial application development is essential. However, considering the large amount spatial data for processing and the physical limitation of today’s mobile devices in relation to battery power, CPU speed and data storage etc., it is nearly impossible to accomplish all computation intensive tasks efficiently on the device itself. Therefore, client-server architecture is usually a preferred option where the mobile devices act as clients sending requests and receiving responses whilst the calculation modules are wrapped and delivered in the form of web services. In this case, on the server side, the geospatial applications can take advantages of the capability of a variety of spatial databases back-ends, including spatial indexing, searching and retrieving. There are now a variety of Python modules, libraries and open sources etc. available for fast constructing and deploying web services for mobile geospatial applications. This talk attempts to give an introduction on using Python for building RESTful web-services for mobile geospatial application development, including back-end spatial database access and spatial information retrieval.