Sigma xi showcase 2013

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Sigma xi showcase 2013

  1. 1. Antioxidative Effects on MaleFertility: Roles of reactive oxygenspecies (ROS) on D. melanogasterfertility and sperm viability Weily Lang, Preethi Radhakrishnan CUNY LaGuardia Community College
  2. 2. HYPOTHESIS• We hypothesized that when flies are fed antioxidants, sperm viability and fertility will increase because the antioxidants accumulated within gametic tissue will help rescue cells from prolonged paraquat- induced ROS damage.
  3. 3. COLLECTED VIRGINS STARVED MALES FOR 24 ISOLATED HOURS FEMALES FEED DIETS WITH TREATMENTS:MELATONIN LIPOIC ACID ETHANOL(Antioxidant) (Antioxidant) (Control) MATING PARAQUAT ASSAULT & Copula (induce ROS) Duration SPERM FERTILITY VIABILITY (Progeny Counts)
  4. 4. RESULTS
  5. 5. Results: Mating Probability 100 80% Mated 60 40 20 M NM 0 ETHANOL MELATONIN LIPOIC ACID
  6. 6. Results: Copula Duration 30Duration of mating (min) 25 20 15 10 5 0 ETHANOL MELATONIN LIPOIC ACID
  7. 7. Results: Sperm Viability 0.9Proportion of Live Sperm 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 ETHANOL MELATONIN LIPOIC ACID
  8. 8. Results: Fertility 120Total Number of Offspring 100 80 Produced 60 Male Female 40 20 0 ETHANOL MELATONIN LIPOIC ACID
  9. 9. Conclusion• Mating probability and Copula Duration: − Antioxidant- fed treatments had no influence in male to female attraction or mate guarding behavior. − This indicates that feeding antioxidants does not significantly influence pre- copulatory behaviors• Sperm viability: − Males fed Melatonin and Lipoic acid had a significantly higher proportion of live sperm (74% and 56%) than the Ethanol treatment (32%). − This indicated that both antioxidants Melatonin and Lipoic Acid, might protect sperm from the lipid peroxidation activity of Paraquat• Fertility: − Males fed Melatonin and Lipoic acid sired significantly more offspring (mean = 187 and 148) the Ethanol treatment (mean = 82). − This shows that antioxidant-fed males, passed more viable sperm to females than the Ethanol treated flies, thereby siring more offspring.
  10. 10. References1) Bonilla, E., et al., 2006. Paraquat –induced Oxidative stress in D. melanogaster: Effects of Melatonin, Glutathione, Serotonin, Minocycline, Lipoic Acid and Ascorbic Acid. Neurochem Res, 31:1425-14322) Bonilla, E, et al., 2002. Extension of life-span and stress resistance of D.melanogaster by long-term supplementation with melatonin. Experimental Gerontology, 37:629-638.3) Dowling, D, Simmons, L.W. 2009. Reactive oxygen species as universal constraints in life-history evolution. Proc. R. Soc. B. 276: 1737-1745.4) Aitken, J., Roman, D, S., Antioxidant Systems and Oxidative stress in the testes. 2008. Molecular mechanisims in Spermatogenesis.5) Radhakrishnan, P., Fedorka, K.MF. 2011. Influence of female age, sperm senescence and multiple mating on sperm viability in female D. melanogaster. 57: 778-836) Radhakrishnan, P., Taylor, P.W. 2007. Seminal fluids mediate sexual inhibition and short copula duration in mated female Quuensland fruit flies. 53: 741-745.
  11. 11. Acknowledgements• Dr. Preethi Radhakrishnan• NIH-Bridges R25 PAR-11-285• Dr. Thomas Onorato• Cheryl Pinzone, University of Georgia• Judith O’Toole, CUNY LaGuardia Community College
  12. 12. Questions& Comments

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