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Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
Biotech 2011-03-prokaryotic-genes
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Biotech 2011-03-prokaryotic-genes

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    • 1. 8 Microbial Genetics
    • 2.
      • Genetics: The study of what genes are, how they carry information, how information is expressed, and how genes are replicated.
      • Gene: A segment of DNA that encodes a functional product, usually a protein.
      Terminology
    • 3.
      • Genome: All of the genetic material in a cell
      • Genomics: The molecular study of genomes
      • Genotype: The genes of an organism
      • Phenotype: Expression of the genes
      Terminology
    • 4. E. coli Figure 8.1a
    • 5. Chromosome Map Figure 8.1b
    • 6. Flow of Genetic Information Figure 8.2
    • 7. DNA
      • Polymer of nucleotides: Adenine, thymine, cytosine, and guanine
      • Double helix associated with proteins
      • "Backbone" is deoxyribose-phosphate
      • Strands are held together by hydrogen bonds between AT and CG.
      • Strands are antiparallel.
      Figure 8.3b
    • 8. DNA Figure 8.3a
    • 9. DNA Figure 8.4
    • 10. DNA
      • DNA is copied by DNA polymerase
        • In the 5'  3' direction
        • Initiated by an RNA primer
        • Leading strand is synthesized continuously
        • Lagging strand is synthesized discontinuously
        • Okazaki fragments
        • RNA primers are removed and Okazaki fragments joined by a DNA polymerase and DNA ligase
    • 11. Important Enzymes Table 8.1
    • 12. DNA Replication Figure 8.5
    • 13. DNA
      • DNA replication is semiconservative.
      Figure 8.6
    • 14. Transcription
      • DNA is transcribed to make RNA (mRNA, tRNA, and rRNA).
      • Transcription begins when RNA polymerase binds to the promotor sequence
      • Transcription proceeds in the 5'  3' direction
      • Transcription stops when it reaches the terminator sequence
      PLAY Animation: DNA Replication
    • 15. Transcription Figure 8.7 (1 of 2)
    • 16. Transcription PLAY Animation:Transcription Figure 8.7 (2 of 2)
    • 17. RNA processing in Eukaryotes Figure 8.11

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