Grain size analysis by using ImageJ

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Hướng dẫn sử dụng phần mềm ImageJ để tính toán kích thước hạt (grain, particle size)

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Grain size analysis by using ImageJ

  1. 1. Analyze Particlesby using imageJ<br />http://rsb.info.nih.gov/ij/docs/menus/analyze.html#ap<br />By Nguyen Hoang Viet, PhD.<br />
  2. 2. Content<br />Where to download<br />How to run ImageJ<br />Image Processing & Calculation<br />
  3. 3. Visit this link:<br />http://rsbweb.nih.gov/ij/download.html<br />Note: please check your window first, 32- or 64-bit system<br />Where to download<br />
  4. 4. First – Run ImageJ program and Load the your image file.<br />How to run ImageJ<br />
  5. 5. Open Image File<br />Run ImageJ: Start/All Programs/ImageJ/<br />Open Image File: File/Open…, choose the file and click OK<br />
  6. 6. Image Processing & Calculation<br />
  7. 7. Step 1 – Convert to 8-bit & Crop<br />Open Image File: File/Open…, choose the file and click OK - Grain Size Calculation.jpg<br />Convert to 8-bit: Image/Type/8-bit.<br />Crop: Draw a rectangular shape then Image/Type/Crop.<br />Save the cropped file: File/Save as/Jpeg… - Grain Size Calculation-Crop.tif<br />
  8. 8.
  9. 9. Measurement scale unit<br />Draw a rectangular shape fits on scale bar. You will see its width to be 237 px.<br />So, 1 m equals 79px.<br />
  10. 10. Step 2: Convert to grainy image<br />Image/Adjust/Threshold:<br />Other processing:<br /><ul><li>Process/Binary/Erode
  11. 11. Process/Binary/Dilate </li></li></ul><li>Step 3: Analyze Particles<br />Analyze/Analyze Particles …<br />There are two data files:<br />Results.xls<br />Summary.xls<br />Average grain size:<br />708 px2= 0,113m2.<br />
  12. 12. How to set new scale bar<br />Analyze/Set Scale<br />Then, the unit in imageJwill be µm (micrometer)<br />
  13. 13. Step 4: Analyze data <br />Based on two data files in Excel format, you should find a software for doing further analysis. Such software like: Excel, MatLab, Origin, SciLab*, SciDAVis* …<br />*: free software<br />
  14. 14. Step 4: Analyze data (2) <br />On the “Results” dialog. Choose Results/Distribution… the set bin number for ‘specify bins’ field: 10, 50 and 100.<br />Based on Histogram dialogClick on List to get data list,then you should use other software to draw the graph.<br />
  15. 15. Terms: What is bin(s)?<br />In a particle size distribution measurement, typically each distribution, whether is displayed cumulatively or differentially, is composed of a few hundred data points in a pre-set size range. Each data points is called a bin. Since every particle is measured, each bin is a collection of particles in the size range that bin represents. Depending on the distribution broadness, the total size range can be reset to a finer division, therefore showing the distribution details, i.e., each bin can be pre-set to cover a smaller size range. Other advantages include fine differential between two particles and more accurate statistic values calculated from the distribution.<br />More at http://www.spectrex.com/html_files/practanl.html<br />
  16. 16. Histograms of the grain sizes<br />
  17. 17. Grain sizes of cropped images<br />
  18. 18. Acknowledgement <br />Thank my students, ATP k52 (Fall 2010), for doing well this homework and have good suggestion for further processing.<br />
  19. 19. Any questions? <br />Please email me at viet4777@gmail.com for further information<br />Discussion time!<br />
  20. 20. Widescreen Presentation<br />Even if you do not have a widescreen display, you can still create and present 16:9 slides. PowerPoint’s Slide Show always resizes your slides to fit any screen.<br />This template is formatted with a 16:9 “widescreen” aspect ratio. It’s a great way to take advantage of laptops, televisions and projectors equipped with widescreen displays.<br />
  21. 21. Widescreen Advantages<br />Side by side material fits more naturally.<br />Widescreen adds drama to graphics and images.<br />
  22. 22. Widescreen Graphics<br />Even a single graphic, such as a chart, can be presented more dramatically in widescreen.<br />
  23. 23. Pictures can also be presented more dramatically in widescreen.<br />Widescreen Pictures<br />
  24. 24. Creating 16:9 Presentations<br />To setup a widescreen presentation, do one of the following:<br />Start with this template. Simply delete the example slides and add your own content.<br />Or, go to the Design tab and open the Page Setup Dialog. Click the Slide Sizedropdown and pick On-screen Show (16:9) (Note: we also support 16:10, which is a common widescreen laptop resolution. )<br />Important: Always start with your slide size set to the aspect ratio you intend to use. If you change the slide size after you’ve created some slides, your pictures and other graphics will be resized. This could potentially distort their appearance.<br />
  25. 25. Slide Show Tips<br />To present in true widescreen, you’ll need a computer and, optionally, a projector or flat panel that can output widescreen resolutions.<br />Common computer widescreen resolutions are 1280 x 800 and 1440 x 900. (These are 16:10 aspect ratio, but will work well with 16:9 projectors and screens.)<br />Standard high definition televisions resolutions are1280 x 720 and 1920 x 1080. <br />Use the Test Pattern on the next slide to verify your slide show settings.<br />
  26. 26. Widescreen Test Pattern (16:9)<br />Aspect Ratio Test<br />(Should appear circular)<br />4x3<br />16x9<br />

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