Once your semen is in the syringe and the syringe is properly protected in the shirt or coverall, place a paper towel or two in the pocket on the side away from the gloved hand.
Using the tail as leverage, insert your cupped gloved hand into the cow.
Once your hand is in, take the paper towel and remove manure or debris from the vulva.
As we learned in Chapter 4, insert the syringe at a 45 degree angle, level off, move forward through the vagina and cervix, stopping the syringe with your index finger at the target.
Once on target slowly deposit the semen, by pushing the plunger forward. I like to use my two middle fingers to hold the syringe while pushing the plunger with my thumb, that way I don’t have a tendency to pull the syringe back off target. Five seconds is enough time to say “Another fine calf from an ABS progeny tested sire”! Rushing the semen out of the straw too fast can harm them. The objective is to get them all in there while capable of fertilizing the egg.
With your gloved hand pull the straw and sheath from the syringe, put the syringe back in your shirt or coverall, spread the fingers on your ungloved hand, place them inside the sleeve and peel it down over the sheath. This makes for a neat, not messy, package that is easily disposed of.
Do it while it’s fresh in your mind.
Used gloves and sheaths don’t look very nice hanging on the fence or laying around the yard.
Anything that kills germs kills sperm.
Just good business.
Lets review the equipment needed.
A review of how to handle the straw.
Transcript of "Ai manual chapter 05 part 2"
Step 30 <ul><li>Put on Plastic, Shoulder-length Glove on Arm That Will be Working in Cow </li></ul><ul><li>Make Sure it’s Stretched Completely up Arm and Fingers are Well-filled and Fairly Tight </li></ul><ul><li>Have Two or Three Clean Paper Towels Opened in Your Pocket </li></ul>
Step 31 <ul><li>Pick up Cow’s Tail and Move it Toward the Outside of the Arm That Will Enter the Cow </li></ul><ul><li>Use Hand That Does Not Have the Glove </li></ul><ul><li>Use a Paper Towel to Avoid Direct Contact With Manure and Genital Discharge </li></ul>
Step 32 <ul><li>With the Hand Outside the Cow, Wipe the Area Around the Vulva and Inside Lips With a New, Dry Paper Towel </li></ul>
Step 33 <ul><li>By Exerting a Slight Backward and Downward Pressure With the Wrist Inside the Cow, You Can Open the Vulva Lips to Make Syringe Insertion Easier </li></ul><ul><ul><li>Keeps the Syringe Clean From External Contamination </li></ul></ul>
Step 34 <ul><li>When the Syringe Tip is on Target, Slowly Depress Plunger </li></ul><ul><ul><li>Should Take 5 Seconds to Completely Empty Inseminating Straw </li></ul></ul><ul><ul><li>Be Sure to Push the Plunger Forward and Not Pull the Syringe Back Away From the Target </li></ul></ul>
Step 35 <ul><li>After Semen is Deposited Slowly Withdraw the Syringe and Your Arm </li></ul><ul><li>Release the Sheath Containing the Straw From the Syringe </li></ul><ul><ul><li>Hold it in Your Gloved Hand </li></ul></ul><ul><li>Peel Glove Down Over Them </li></ul>
Step 36 <ul><li>Be Sure to Complete the Breeding Record Immediately After Insemination </li></ul><ul><li>Record Sire Identification and Collection Code if You Didn’t Earlier </li></ul><ul><ul><li>Can be Read Through Used Glove, but Maybe Difficult </li></ul></ul>
Step 37 <ul><li>After Completing the Breeding Record, Break the Sheath Inside the Glove </li></ul><ul><li>Dispose of Used Materials Immediately and Properly </li></ul><ul><li>Never Reuse Gloves or Sheaths </li></ul>
Step 38 <ul><li>Clean Reusable Equipment After Each Cow/Heifer </li></ul><ul><ul><li>Syringe and Cito Cutter </li></ul></ul><ul><ul><li>Rinse With Water if Necessary </li></ul></ul><ul><ul><li>NEVER Use Disinfectants or Soaps </li></ul></ul><ul><ul><li>Wipe the Syringe With 70% Ethyl or Isopropyl Alcohol </li></ul></ul><ul><ul><ul><li>Pay Special Attention to the “O” Ring and Upper Parts of Both Syringes </li></ul></ul></ul>
Step 39 <ul><li>Before Leaving Insemination Area, Always Discard Your Trash and Disinfect Your Footwear and Bottom of Your Inseminating Kit With a Disinfectant </li></ul><ul><ul><li>Will Help to Prevent/Eliminate Disease Transfer From Farm to Farm or to Your Own Cattle </li></ul></ul>
Summary <ul><li>When Ready to Inseminate: </li></ul><ul><ul><li>Transfer a Single Straw From Refrigerator to Thaw Water Quickly </li></ul></ul><ul><ul><li>Water Temp Must be Between 94 - 98 Degrees Fahrenheit (35 - 37 Degrees Celsius) </li></ul></ul><ul><ul><li>Keep Straw in Water for at Least 30 Seconds, but No More Than 15 Minutes </li></ul></ul><ul><ul><li>Make Sure Syringe is Properly Warmed Before Inserting Semen Straw </li></ul></ul><ul><ul><li>Frequently Check Calibration of Thaw Water Thermometers </li></ul></ul>
Question 1 <ul><li>Why is it important to check the insemination record first, before inseminating any cow? </li></ul>To see if she had been inseminated before and could be pregnant, or if her heat is on a normal cycle.
Question 2 <ul><li>Why are frequent checks of the dial thermometer necessary? </li></ul>The calibration can get out of adjustment, resulting in a temperature too high or too low for optimum semen recovery.
Question 3 <ul><li>Safety glasses and gloves should be worn each time you open a liquid nitrogen refrigerator. Why? </li></ul>Glasses to protect from spitting nitrogen, or exploding straw. Gloves to protect from nitrogen burns.
Question 4 <ul><li>Will any thawing procedure (including pocket and air) work optimally for ABS straws? </li></ul>No
Question 5 <ul><li>At what temperature must water be maintained for thawing ABS straws? </li></ul>Optimum thawing is at a water temperature of 94 to 98 degrees Fahrenheit.
Question 6 <ul><li>How soon can you remove an ABS straw from the warm water thaw? </li></ul>It takes 30 seconds for the semen to thaw properly.
Question 7 <ul><li>What is the maximum length of time that an ABS straw should be left in the warm water thaw? </li></ul>15 minutes
Question 8 <ul><li>When you’re looking for a straw from a specific bull, how high can you safely raise the canister in the refrigerator? </li></ul>Just high enough in the neck of the refrigerator to be able to read the numbers on the top of the ABS Semen racks.
Question 9 <ul><li>What is the time limit for holding a canister of semen in the refrigerator’s neck tube before possible damage will result? </li></ul>10 seconds
Question 10 <ul><li>Why is it important to warm an inseminating syringe prior to loading it? </li></ul>To eliminate cold shocking the sperm cells.
Question 11 <ul><li>Describe the sheath or sheaths that can be used with the two styles of syringes. </li></ul>Split sheaths for use with “O”ring Syringes. Unsplit sheaths for use with Spiral Syringes.
Question 12 <ul><li>Is the procedure the same for loading all types of sheaths and syringes? </li></ul><ul><li>Compare. </li></ul>No “ O” Ring Syringe and sheath with no adapter: Put the straw in the syringe and pull the sheath into place. If using a split sheath with a green adapter you must set the straw in the adapter then insert the straw and pull the sheath in place. With a spiral syringe the straw must be seated in the white adapter and then inserted in the syringe pulling the sheath down and screwing it on.
Question 13 <ul><li>If the sheath you’re using contains a plastic adapter, do you set the cut end of the straw in it first or do you load the straw in the syringe first? </li></ul>You insert the cut end into the adapter first.
Question 14 <ul><li>When you’re working with your own animals or those of others, why should you be concerned about disinfecting footwear and equipment? </li></ul>Disinfecting all footwear and equipment is important in reducing/eliminating the transfer of diseases from cow to cow and farm to farm.