Fly

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Fly

  1. 1. – –)
  2. 2. C30H37N4SO9 5 ug/ml
  3. 3. - -
  4. 4. - - -
  5. 5.
  6. 6.
  7. 7. – Nutrient agar emended with 1% yeast extract. Nutrient agar emended with 5% sheep blood MaConkeys agar Starch nitrate agar Tryptose blood agar Staphylococcus media (CFU 1944 Holt et al., 2004 Honda et al
  8. 8. ––
  9. 9. – Bioautographictechnique pHThin layer and column chromatography Spectroscopy (UV) Spectrophotometer (IR) Mass spectral Data Hp mudel MS 5988
  10. 10. –Agar Diffusion Method
  11. 11. Table (1): The viable plate count of bacterial flora (CFU/ml) isolated from wings of P. papatasi, M. stabulans, M. domestica and C. pipiens Medium P. papatasi M. stabulans M. domestica C. pipiens. Right Left Right Left Right Left Right Left wing wing wing wing wing wing wing wing Nutrient agar 5 x 102 2 x 102 2.9 x 3.4 x 5.1 x 5.1 x Nil Nil with y. extract 102 102 103 103 Nutrient blood 6 x 102 1 x 102 6.7 x 5.9 x Nil 4.3 x 3 x 102 Nil 103 103 103 MacConkey Nil Nil 3.9 x 3.9 x Nil Nil Nil Nil 103 103 Starch nitrate 1.7 x 102 Nil 5 x 102 4.8 x Nil Nil Nil Nil 102 Tryptose blood 1 x 102 Nil 3.1 x 2.7 x 3.3 x 3.5 x 1 x 102 1.4 x 103 103 103 103 102 Staphylococcus Nil Nil Nil Nil Nil Nil Nil Nil
  12. 12. Table (2): Organisms isolated from wings of the sandfly, the false stable fly, the house fly and the mosquito. Symbole Organism 175b Salmonella arizona 157y Erwina herbicola 68S Yeast 165y Bacillus subtilis 181y Yeast 191T Actinomycete 88T Bacillus circulans 132T Staphylococcus aureus 127T Lactobacillus animalis 98y Bacillus mycoides 113M Pseudomonas aeruginosa 201T Lactobacillus gasseri
  13. 13. Table (3): Antagonistic action of bacterial species between each other grown on nutrient broth amended with yeast extract.Organism S. arizona E. B. B. S. L. B. mycoides P. aeruginosa L. gasseri 175b herbicola subtilis circulans aureus animalis 98y 113M 201T 157y 165y 88T 132T 127TS. arizona x –ve +ve +ve +ve 2+ve –ve +ve +ve175bE. herbicola –ve x +ve –ve –ve –ve –ve –ve –ve157yB. subtilis –ve +ve x +ve 2+ve 3+ve +ve –ve +ve165yB. circulans –ve –ve –ve x +ve 2+ve –ve +ve –ve88TS. aureus –ve –ve +ve +ve x 3+ve –ve +ve –ve132TL. animalis –ve –ve –ve +ve –ve x –ve –ve –ve127TB. mycoides –ve –ve +ve –ve –ve –ve x +ve –ve98yP. –ve –ve –ve –ve –ve –ve –ve x –veaeruginosa113ML. gasseri +ve –ve +ve +ve +ve +ve +ve –ve x201T–ve = no inhibition zone, + = weak inhibition zone, 2+ = moderate inhibition zone, 3+ = good inhibitionzone.
  14. 14. Table (4): Antagonistic action of most potent bacterial species grown on peptone water during log phaseOrganism S. aureus P. aeruginosa B. circulans L. animalis B. subtilis 132T 113M 88T 127T 165yS. aureus x + 4+ + 4+132TP. aeruginosa –ve x –ve –ve –ve113MB. circulans ve + x 3+ +88TL. animalis –ve + 2+ x 2+127TB. subtilis + + 4+ 2+ x165y–ve = no inhibition zone, ve = doubolful inhibition zone, + = weak inhibitionzone, 2+ = moderate inhibition zone, 3+ = good inhibition zone, 4+ = very good
  15. 15. Table (5): Bioautography and migration (Rf) of the active metabolite 88T with various developing solventsDeveloping solvent system Rf value. 0.00Petroleum etherBenzene (saturated with water) 0.00Chloroform (saturated with water) 1.00Carbon tetrachloride (saturated with water) 0.75Methanol 0.85N–Butanol (saturated with water) 0.80Acetone 0.45Diethyl ether 0.55Ethyl acetate 0.50Amyl acetate 0.003% ammonium chloride 0.10N–Butanol : pyridine : water (1 : 0.6 : 1) 0.00N–Butanol : Acetic acid : water (2 : 1 : 1) 0.00Distilled water 0.20
  16. 16. Table (5): Bioautography and migration (Rf) of the active metabolite 88T with various developing solventsMethylene chloride (1 : 1)Developing solvent system Rf valu ePetroleum ether 0.00Benzene (saturated with water) 0.00Chloroform (saturated with water) 1.00Carbon tetrachloride (saturated with water) 0.75Methanol 0.85N–Butanol (saturated with water) 0.80Acetone 0.45Diethyl ether 0.55Ethyl acetate 0.50Amyl acetate 0.003% ammonium chloride 0.10N–Butanol : pyridine : water (1 : 0.6 : 1) 0.00N–Butanol : Acetic acid : water (2 : 1 : 1) 0.00Distilled water 0.20
  17. 17. Table (6): The MIC of active metabolite 88T Test organism MIC ( g/ml) Reference strains: Bacillus subtilis NCTC 8236 <5 Bacillus pumilus NCTC 8241 <5 Micrococcus luteus ATCC 9341 12 Staphylococcus aureus NCTC 7447 12 E. coli BPP01 16 Pseudomonas aeruginosa ATCC 10145 83 Klebsiella pneumonia NCIB 9111 18 Candida albicans IMRU 3669 94 Saccharomyces cerevisiae CBS 1171 94
  18. 18. Table (6): The MIC of active metabolite 88TAspergillus niger LTU 131 >100Local isolates:Bacillus subtilis 165y <5Bacillus mycoides 98y <5Staphylococcus aureus 132T <5Lactobacillus animalis 127T 32Lactobacillus gasseri 201T 40Salmonella arizona 175b <5Erwina herbicola 157y >100Pseudomonas aeruginosa 113M >100Yeast 181y >100Yeast 68y >100
  19. 19.  -B.subtilis cause ocular infection - B. pumilus - B. anthracis - Vibro cholerae - Salmonella - B. dysentri, Amoebic dysentri - - Poliomylitis - (HAV) A - Yaws - Infentile diarrhea
  20. 20.  Pseudomonas aeryginosa Staphylococcus aureus Furuncles (boils) Carbuncles Impetigo Cystitis
  21. 21.  Enterocolitis Osteomylitis Bacteremia. Cerebritis. Abscesses of muscle, skin. Urinogenital tract. Central Nervous system. Various intraabdominal organs. Food poisoning.
  22. 22. (Spore forming) Lactobacillus  Lactoperoxidase system   Lactobacilli(Vincent, Veomell and Riley 1959) (Gram-ve bacteria)
  23. 23.  (Spore forming) Bacillius (The prokaryotes vol. II) proteolysis power Bacilli polymyxin, gramicidin, Bactiracin Several proteinases, amylase, subtilysin B. circulus 2000
  24. 24.  Ampusa muscae (Gram-ve,+ve) (Gram-ve,+ve)
  25. 25.  Sarcophaga Aspergillus sp. B. circulus (1997) Ml.Wt (2002) 26kDa.
  26. 26. Fig. (1): A simplified scheme for the extraction, isolation and purification of the active metabolite 88T biosynthesized by Bacillus circulans 88T.
  27. 27. Fig. (2): A simplified scheme for the extraction, isolation and purification of the active metabolite 88T biosynthesized by Bacillus circulans 88T
  28. 28. Fig. (3): Mass spectrum of the active metabolite 88T
  29. 29. Fig. (4): IR spectrum of the active metabolite 88T .
  30. 30. Fig. (5): 1H–NMR spectrum of the active metabolite 88T
  31. 31. Fig. (6): The time killing curves of the active metabolite 88T using Bacillus mycoides 98y, Bacillus subtilis 165y, Staphylococcus aureus 132T and Salmonella arizona 175b.
  32. 32.  B.circulans
  33. 33.
  34. 34. 
  35. 35. 5 mg/ml
  36. 36.
  37. 37.  ...

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