Can Aspargausinhibit population growth of yeast cells? Tiffany Sato Experium Science Academy Marlborough School, Ninth Grade
Hypothesis and Goals During digestion, asparagus produces sulfur compounds, which is beneficial in the reduction of the growth of cancer cells (Mitchell, 2001). Therefore, I decided to investigate this further which led me to the information that asparagus produced high levels of sulfur. From this, I decided to start studying the effect of asparagus on yeast activity. I wanted to see a decrease in yeast activity compared to a negative and positive control. A decrease in growth meant that the asparagus, the high amounts of sulfur, was inhibiting the growth of the yeast cells.
Why I used Yeast Cells and the Similarities Yeast Cells Cancer Cells (Saccharomyces Typical eukaryotic cerevisiae) structure Typical eukaryotic Fast and structure incontrollable cell Fast and division incontrollable cell division Economically accessible Genetic material is known
Yeast Cells as a Model Organism I realized that scientific studies have shown thatSaccharomyces cerevisiae could be used as an alternative to cancer cells when doing research. (Hartwell, 2001) (Mortimer, 1973)
Yeast Activity Growthwith SpectrophotometerGrow S. cerevisiae Centrifuge all Incubated all in YPD+, YPD- the different cultures for 24 hours , YPDAsp Broths mediums Measured the absorbance of each using a Compared the Yeast spectrophotometer at Activity Growth Results 600nm
Viable Colony Counts using Agar Plates For my agar plates, I Streak onto my three different inoculated a Incubate for 48 plates:colony from a hours at 30oC positive, negative colony off a and experimentalpositive plate. Incubate for 24 Record Viable Colony hours at 30o C Count Results
Data Average for Negative (without any nutrients) : 0.189 abs Average for Experimental (with asparagus): 0.488 abs Average for Positive (with nutrients, without asparagus):0.633 abs
Conclusions for Yeast Growth Activity My results from my experiment with the liquid medium with asparagus had the lowest absorbance (0.488 abs) compared to my positive control (0.633 abs ) and higher absorbance compared to my negative control (0.189 abs). This indicates a decrease in yeast activity upon addition of asparagus indicating minimal cell growth.
Comparison of the ControlsNegative Experiment Positive Experiment
Conclusions from Viable Colonies Although viable colonies have limited quantification, the pictures show the apparent difference of structural colonies between the Positive, Experimental, and Negative. Positive Experimental Negative • Raised, Corvex • Flat Surface • Produced no Surface colonies
Acknowledgements Thank you to Sigma Xi for this opportunity. Thank you to RaudhahRahman, ExperiumScience Academy, my parents, and those who have supported me throughout.