Cancer Cells
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Cancer Cells

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Cancer Cells Cancer Cells Presentation Transcript

  • Can Aspargausinhibit population growth of yeast cells? Tiffany Sato Experium Science Academy Marlborough School, Ninth Grade
  • Hypothesis and Goals During digestion, asparagus produces sulfur compounds, which is beneficial in the reduction of the growth of cancer cells (Mitchell, 2001). Therefore, I decided to investigate this further which led me to the information that asparagus produced high levels of sulfur. From this, I decided to start studying the effect of asparagus on yeast activity. I wanted to see a decrease in yeast activity compared to a negative and positive control. A decrease in growth meant that the asparagus, the high amounts of sulfur, was inhibiting the growth of the yeast cells.
  • Why I used Yeast Cells and the Similarities Yeast Cells  Cancer Cells (Saccharomyces  Typical eukaryotic cerevisiae) structure  Typical eukaryotic  Fast and structure incontrollable cell  Fast and division incontrollable cell division  Economically accessible  Genetic material is known
  • Yeast Cells as a Model Organism I realized that scientific studies have shown thatSaccharomyces cerevisiae could be used as an alternative to cancer cells when doing research. (Hartwell, 2001) (Mortimer, 1973)
  • Research Methods
  • Can Asparagus inhibit population growth in yeast cells? Yeast Activity Viable Colony Growth with Counts with AgarSpectrophotometer Plates
  • RecipesPositive (YPD+)• 1% Yeast Extract• 2% Peptone• 1% DextroseNegative (YPD-)• WaterExperimental (YPDAsp)• 1% Yeast Extract• 2% Peptone• 1% Dextrose• 50mL of Filtered Asparagus
  • Yeast Activity Growthwith SpectrophotometerGrow S. cerevisiae Centrifuge all Incubated all in YPD+, YPD- the different cultures for 24 hours , YPDAsp Broths mediums Measured the absorbance of each using a Compared the Yeast spectrophotometer at Activity Growth Results 600nm
  • Viable Colony Counts using Agar Plates For my agar plates, I Streak onto my three different inoculated a Incubate for 48 plates:colony from a hours at 30oC positive, negative colony off a and experimentalpositive plate. Incubate for 24 Record Viable Colony hours at 30o C Count Results
  • Results for Yeast Growth Activity
  • Absorbance of Yeast Activity Sample 1 0.9 0.8Absorbance (600nm) 0.7 0.6 0.5 POSITIVE 0.4 NEGATIVE 0.3 EXPL 0.2 0.1 0 0 5 10 15 Sample Numbers
  • Data Table
  • Data Average for Negative (without any nutrients) : 0.189 abs Average for Experimental (with asparagus): 0.488 abs Average for Positive (with nutrients, without asparagus):0.633 abs
  • Conclusions for Yeast Growth Activity My results from my experiment with the liquid medium with asparagus had the lowest absorbance (0.488 abs) compared to my positive control (0.633 abs ) and higher absorbance compared to my negative control (0.189 abs). This indicates a decrease in yeast activity upon addition of asparagus indicating minimal cell growth.
  • Results for Viable Colonies
  • ExperimentalResults
  • Comparison of the ControlsNegative Experiment Positive Experiment
  • Conclusions from Viable Colonies Although viable colonies have limited quantification, the pictures show the apparent difference of structural colonies between the Positive, Experimental, and Negative. Positive Experimental Negative • Raised, Corvex • Flat Surface • Produced no Surface colonies
  • Acknowledgements Thank you to Sigma Xi for this opportunity. Thank you to RaudhahRahman, ExperiumScience Academy, my parents, and those who have supported me throughout.