Biological Solutions in a chemical world

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A central working hypothesis at Novozymes is that sugar/biomass can gradually & partially replace fossil feedstock

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Biological Solutions in a chemical world

  1. 1. BIOLOGICAL SOLUTIONS IN A CHEMICAL WORLDThomas SchäferVice President, R&DPhiladelphia, August 2012
  2. 2. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovozymes – The World Leader in Bioinnovation• Largest market share of all players in Global enzyme market Industrial Enzymes (47%) 2009 value: DKK ~ 16bn• More than 60 years legacy in the Novozymes Danisco DSM Others Captive business• 2011 sales of DKK 10,510m 47% 21% 6% 16% 10% (+8% in DKK, +10% in LCY, +7% organic) 0% 20% 40% 60% 80% 100%• 20 years sales growth CAGR of 8%• EBIT: DKK 2340m (+11 %)• ~ 14% of sales spent on R&D• + 6,300 granted and pending patents• + 5,500 employees Enzyme Business BioBusiness• Global Organisation Household Food & Feed & Micro- Biopharmaceutical Bioenergy• More than 700 products used in 130 Care Beverages other Tech. organisms ingredients countries in over 30 different industries *A+B shares April, 2011 2
  3. 3. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovozymes’ Vision • Less need for energy, water and chemicals • Better utilization of raw materials • Less CO2 emissions • Ability to convert agricultural feedstocks • Ability to produce food, feed, fibres, fuels & materials 3
  4. 4. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferCentral Working Hypothesis: Sugar/biomass cangradually & partially replace fossile feedstocks Fuel Chemicals Energy … • Where it “makes sense” (economy, LCA, customers) • Where it is technically advantagous 4
  5. 5. 5 NOVOZYMES PRESENTATION Biorefinery as central element
  6. 6. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferSummary: Novozymes technologies deliver key enablers for the biobased society 2 REFINING 1 INPUT 3 OUTPUT The Sugar Platform Microbial products for BioAg Platform Enzymes Chemicals 5 4 Strong partnerships from agriculture to consumer companies 6
  7. 7. 7 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer1 INPUT: Three main technology BioAg platforms Rhizobia Biofertility • Improve the uptake of N and P Biocontrol • Fungicides and bactericides • Insecticides • Herbicides • Nematicides (future interest) LCO: OBSERVED BENEFITS Bio-yield enhancement • Yield improvement unrelated to macronutrient acquisition
  8. 8. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer2 Novozymes’ Central Technology: The Sugar Platform • Cellulosic bioethanol drives our technology development Starch Enzyme process Fermentable sugars Yeast Bio- Fermentation ethanol Waste Pre- Enzyme treatment Cellulosebiomass process process 8
  9. 9. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferFeb 2010: Novozymes Launches Cellic® CTec2Allows production offuel ethanol frombiomass @ ca. USD2.50 per gallon,depending onprocess 9
  10. 10. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferFeb 2012: Next generation Cellic CTec3 ® We can secure your plant’s lowest total cost 10
  11. 11. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferFeb 2012: Next generation Cellic CTec3 ® 11
  12. 12. THE TECHNOLOGY IS READY ANDPRODUCTION IS BEING SCALED UP 2010 2011 2012 2013 2014 US Demonstration scale Commercial scaleEurope Demonstration scale Commercial scale China Pilot scale Demonstration scale Commercial scale Brazil Pilot scale Demonstration scale SELECTED EXAMPLES 12
  13. 13. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferM&G Breaks Ground for First Commercial Plant inEUROPE • Novozymes’ partner M&G begins construction of the world’s first commercial-scale cellulosic ethanol plant • The plant will produce 13 million gallons of ethanol per year from biomass, and is competitive with 60- 70 USD/barrel oil • The biofuels plant will be ready in 2012, and is located in Crescentino, Northern Italy Pictures courtesy of M&G 14
  14. 14. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer…while China accelerates:Shengquan Group in July 2012… • When China’s Shengquan Group starts producing cellulosic ethanol in July 2012, it will be the first in the world to do so on a commercial scale.Novozymes’ partner Shengquan • More than that, the production will also invests $100 million to market be cost-competitive with conventional cellulosic ethanol ethanol, as the feedstock is a waste product from Shengquan’s currentDuring the visit to Denmark of Hu Jintao, production. President of the Peoples Republic of China, Shengquan and Novozymes • Shengquan is a leading producer of finalized an enzyme supply deal to furfural for resin production in the market cellulosic ethanol. foundry industry. Furfural is produced from corncob xylose, with the cellulose from the corncob left behind as a waste15. June 2012 product. Using Novozymes’ enzymes, Shengquan will now be able to converthttp://www.novozymes.com/en/news/news- the cellulose into higher-value sugars archive/Pages/novozymes-partner-shengquan- that can be fermented to ethanol. invests-100-million-to-market-cellulosic-ethanol.aspx 15
  15. 15. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer…as well as Brazil • Today, June 23.05. 2012, GraalBio announced plans to start production in December 2013 of 82 million liters (22Novozymes to supply first advanced million gallons) of advanced biofuels per biofuels factory in Brazil year at a new factory in the Brazilian state of Alagoas.Brazilian bioenergy company GraalBio to construct commercial scale cellulosic • The plant will produce cellulosic ethanol ethanol facility in Brazil by 2013. from sugarcane bagasse and straw.23. June 2012 • Novozymes will supply the necessary enzyme technology • Beta Renewables and Chemtex, both part of Italian chemical group Mossi & Ghisolfi (M&G), will provide other process technologies and engineering.http://www.novozymes.com/en/news/news- http://www.biofuelsdigest.com/bdigest/2012/05/24/graalbio archive/Pages/Novozymes-to-supply-first-advanced- -investimentos-plans-to-invest-724-5-million-in-five- biofuels-factory-in-Brazil.aspx cellulosic-ethanol-projects-in-brazil/ 16
  16. 16. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer3 The Sugar Platform is our link to OUTPUT Starch Enzyme process Fermentable sugars Yeast Bio- Fermentation ethanol Waste Pre- Enzyme treatment Cellulosebiomass process process Platform Technology: enables production of a broad spectrum of products 17
  17. 17. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer3 Production of Renewable Chemicals – a Good Fit to our Technology and our Vision Yeast Bio- Fermentation ethanol Fermentable sugars 18
  18. 18. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer3 Production of Renewable Chemicals – a Good Fit to our Technology and our Vision Yeast Bio- Renewable Fermentation ethanol chemicals Fermentable sugars 19
  19. 19. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer3 Production of Renewable Chemicals – a Good Fit to our Technology and our Vision Yeast Bio- Renewable Fermentation ethanol chemicals Fermentable sugars Heterogenous Renewable catalysis chemicals 20
  20. 20. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer3 Partnering is essential for us Yeast Bio- Renewable Fermentation ethanol chemicals Fermentable sugars Chemical Renewable Catalysis chemicals Novel engineered pathway Renewable -- chemicals Novel Bioprocess/ Enzymes 21
  21. 21. 22 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovozymes’ biochemicals pipeline Acrylic acid Green polypropylene C4 dicarboxylic acids Partner tbdNovozymes’biosolutions forbiochemicals Glycol Glutamic acid HMF/FDCA Partner tbd BY BUILDING A BIOCHEMICAL INDUSTRYPAVING THE WAY FOR THE BIOBASED SOCIETY TOGETHER WITH OUR PARTNERS
  22. 22. 23 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovozymes’ biochemicals pipeline Acrylic acid Green polypropylene C4 dicarboxylic acids Partner tbdNovozymes’biosolutions forbiochemicals Glycol Glutamic acid HMF/FDCA Partner tbdPAVING THE WAY FOR THE BIOBASED SOCIETY HIGHLIGHTS AUGUST 2012
  23. 23. 24 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovozymes/Cargill partner with BASF  August 18th, 2012  Cargill & Novozymes started in 2008  BASF joins the cooperation now
  24. 24. 25 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferCase study: Novozymes’ Malic Acid project Market potential • Market size: 130 mUSD • Market volume: 60 kMT • Annual growth: 4% • Applications: Food, beverages, cleaningProduced from renewables agents Starch or Glucose Malic acid • C4 dicarboxylic acids are platform biomass chemicals: Petro-based Maleic • can be further converted into Malic acid feedstock anhydride derivatives such as BDO, THF and GBL which are used for a wide Price in USD 2000 2010 variety of plastic, polymer and resin applications Malic acid 800/MT 2100/MTSource: ICIS, SRI Consulting
  25. 25. 26 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferOverview of Novozymes’ Malic Acid project Overall objective was to develop a microorganism that produces commercially relevant levels of malic acid and secure freedom to operate by timely filing of IP Approach: Improve malic acid production in the novel, natural malic acid producing filamentous fungus Aspergillus oryzae NRRL 3488 by overexpression of cloned genes and classical mutagenesis Project targets were defined by a minimum volumetric productivity and an overall Performance Factor (= Final product concentration * Volumetric Productivity * g/g Yield on glucose) Target performance must also be achieved in commercially acceptable fermentation mediumMALIC ACID STATUS AUGUST 2012
  26. 26. 27 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer Highest final malic acid concentration (Mutant M3) and highest volumetric productivity (Mutant M4) Major Acids Major Acids 350 300Acid Concentration (g/L) Acid Concentration (g/L) 300 250 250 200 200 150 150 100 100 50 50 0 0 0 24 48 72 96 120 144 168 192 0 24 48 72 96 120 144 168 192 Total C4 Time (hrs) Total C4 Time (hrs) acids acids Malic acid Malic acid  Data from un-optimized lab scale fermentations  Likelihood of further strain improvements is high 27
  27. 27. 28 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferMalic Acid as the preferred C4-platform chemical:RECOVERY - crystal formation in fermentation Mutant M3 Mutant M4 Crystal size varies with strain/lineage Smaller crystals give higher viscosity
  28. 28. 29 NOVOZYMES PRESENTATION ACS 2012 Schäfer Enzymatic modifications relevant for the4 chemical industry: > het/homogenous catalysis! Esterification and ester • Application of lipases & cutinases in aqueous or non- aqueous environment. Can be enantioselective. Also used for hydrolysis polyester synthesis. Nitrile hydrolysis • Hydrolysis to amide or carboxylic acid under mild conditions. Can be enantioselective. • Polymerization of aromatic structures by hydrogen Free-radical polymerization abstraction using oxidoreductases • Oxidation of aromatic side chains Side chain oxidation using oxidoreductases • Hydroxylation of aromatic or aliphatic carbon using Hydroxylation oxidoreductases. Can be enantioselective • Epoxidation of double bonds using lipases Epoxidation or oxidoreductases 8/1 9/2 012
  29. 29. 30 NOVOZYMES PRESENTATION ACS 2012 Schäfer Enzymatic modifications relevant for the4 chemical industry: > het/homogenous catalysis! Esterification and ester • Application of lipases & cutinases in aqueous or non- aqueous environment. Can be enantioselective. Also used for hydrolysis polyester synthesis. Nitrile hydrolysis • Hydrolysis to amide or carboxylic acid under mild conditions. • Proteases Can be enantioselective. • Lipases, • Polymerization of aromatic structures by hydrogen Free-radical polymerization esterases/cutinases abstraction using oxidoreductases • Oxido-reductases; laccases, • Oxidation of aromatic side chains Side chain oxidation using oxidoreductases peroxidases, chloroperoxidase, peroxygenase • Hydroxylation of aromatic or aliphatic carbon using Hydroxylation oxidoreductases. Can be enantioselective • Amylolytic enzymes • Epoxidation of double bonds using lipases Epoxidation or oxidoreductases 8/1 9/2 012
  30. 30. 31 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferNovel experimental unspecific peroxygenasecatalyses hydroxylation reactions using onlyhydrogen peroxide• Opportunities: • Peroxygenases provides oxyfunctionality to a range of different substrates Peroxygenases can be used i.e. in: • Hydroxylation reactions • Oxidation reactions • Polymerisation reactions • Regio and stereo selectivity can be very high but depends on the actual substrate • Peroxygenases operates under mild conditions (pH 5-8; 30-50C), but is still relatively stable towards auxiliary Aromatic epoxidation (e.g. naphthalene) chemicals (e.g. cosolvents, detergents etc.) Alkane hydroxylation (e.g. n-hexane) Epoxidation (e.g. styrene) • Only waste product is water Ether cleavage (e.g. Tetrahydrofuran) N-Oxidation (e.g. pyridine) Sulfoxidation (e.g. dibenzothiophene) Hofrichter (2010), Applied Microbiol. Halide oxidation (e.g bromide, Br-) Biotechnology, 87
  31. 31. 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 SchäferConclusions: • The sugar platform is ready for implementation • ethanol will be deployed first • biochemicals catching up…also from biomass • Market pull for renewable chemicals • ”Untraditional partnerships” • combine agriculture, biology & chemistry and building new value chains • new growth markets for grain processors, biotech companies, chemical companies and consumer goods companies 32
  32. 32. 33 8/19/2012 NOVOZYMES PRESENTATION ACS 2012 Schäfer Thank you! tsch@novozymes.com

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