BMI   ATA GLANCE    BY: LOKENDRA YADAV
OBJECTIVES: Origin of bio signals Propagation of bioelectric signal Electrodes Recording of bioelectric signals Analy...
ORIGIN     OFBIO-SIGNALS    ?
ORIGIN OF BIO-SIGNALS                      1 (EEG)                           (EOG                      2    /ERG)         ...
Recording   Stimulating
Array ElectrodesSurface           Inserted      Microelectrodes• Metal   Plate     •Needle   •Solid metal•Suction         ...
Surface: Metal PlateElectrodes  (a)Metal-plate electrode used    for application to limbs.  (b)Disposable foam-pad    elec...
Surface: Suction Electrodes• No   straps or adhesives• Precordial (chest) ECG• Can only be used for     short periods
MicroelectrodesMeasure potential difference across cell membraneRequirements  Small enough to be placed into cell  Stron...
Stimulating Electrodes     Features        – Net current across electrode-electrolyte interface        is not zero        ...
Practical Hints for using anelectrode .....   Electrode and lead wire (exposed to    the electrolyte) must be of the same...
WHAT ARE THE BASICELECTRODE REQUIRED FORRECORDING OF A BIO-POTENTIAL            ?ACTIVE ELECTRODEREFERANCE ELECTRODEGRO...
Active electrode: Actually this is the only electrode which pick the signal from the subjects body.     BUT  HOW ?
WE HAVE TOUNDERSTANDELECTRODE JELLYINTERFACE 1ST
REDOXREACTION?
Electrode – Electrolyte Interface         Electrode                           Electrolyte (neutral charge)                ...
Electrode – Electrolyte Interface                       General Ionic Equations                  a)                  n    ...
Half Cell Potential     A characteristic potential difference established by the     electrode and its surrounding electro...
Measuring Half Cell PotentialNote: Electrode material is metal + salt or polymer selective membrane
CONFUSE?D?    ???
Biopotential Electrodes – The Basics     The interface between the body and electronic      measuring devices     Conduc...
Electrode skin jelly interface                                       ELECTROLYTETO                                       -...
Current Flow at the Electrode-Electrolyte Interface                               Ion- flow          Electrons move in o...
Half-Cell Potential   When metal (C) contacts electrolyte, oxidation (C  C + + e –    ) or reduction (A-  A + e –) begi...
Standard Hydrogen electrodeNote: Ag-AgCl has low junctionpotential & it is also very stable ->hence used in ECG electrodes!
Nernst Equation         When two aqueous ionic solutions of different concentration are         separated by an ion-select...
Equivalent Circuit                Cd       : capacitance of electrode-eletrolyte interface                Rd       : resis...
Electrode Skin Interface                                              Ehe                           Alter skin            ...
Polarization     If there is a current between the electrode and electrolyte, the observed half     cell potential is ofte...
Polarizable and Non-Polarizable    Electrodes                                                                   Use for re...
Motion Artifact    Why    When the electrode moves with respect to the electrolyte, the    distribution of the double laye...
But one thing that should notbe stopped is questioning
E-mail: techyadav@gmail.com, lokendra@technovast.co.inM: +91-9694958872,09868732573
THANK YOU
Electrode Interfacing
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Electrode Interfacing

  1. 1. BMI ATA GLANCE BY: LOKENDRA YADAV
  2. 2. OBJECTIVES: Origin of bio signals Propagation of bioelectric signal Electrodes Recording of bioelectric signals Analysis of recorded bio-electric signal complications
  3. 3. ORIGIN OFBIO-SIGNALS ?
  4. 4. ORIGIN OF BIO-SIGNALS 1 (EEG) (EOG 2 /ERG) 5 (pulse) 4(EMG) 3(EGG)
  5. 5. Recording Stimulating
  6. 6. Array ElectrodesSurface Inserted Microelectrodes• Metal Plate •Needle •Solid metal•Suction •Wire •Supported metal•Floating •Glass micropipette•Flexible
  7. 7. Surface: Metal PlateElectrodes (a)Metal-plate electrode used for application to limbs. (b)Disposable foam-pad electrodes, often used with ECG
  8. 8. Surface: Suction Electrodes• No straps or adhesives• Precordial (chest) ECG• Can only be used for short periods
  9. 9. MicroelectrodesMeasure potential difference across cell membraneRequirements Small enough to be placed into cell Strong enough to penetrate cell membrane Typical tip diameter: 0.05 – 10 microns
  10. 10. Stimulating Electrodes Features – Net current across electrode-electrolyte interface is not zero – The body/electrode has a highly nonlinear response to stimulation Platinum electrodes: – Large currents can cause Applications: neural – Chemical reaction stimulation – Cavitation – Cell damage Steel electrodes for – Heating pacemakers and Applications of stimulating electrodes defibrillators 1. Pacing 2. Ablation 3. Defibrillation
  11. 11. Practical Hints for using anelectrode ..... Electrode and lead wire (exposed to the electrolyte) must be of the same material. Use two similar electrodes when measuring differentials. Lead electrode interface failure. Electrode insulation material. Input impedance of Amplifier.
  12. 12. WHAT ARE THE BASICELECTRODE REQUIRED FORRECORDING OF A BIO-POTENTIAL ?ACTIVE ELECTRODEREFERANCE ELECTRODEGROUND ELECTRODE
  13. 13. Active electrode: Actually this is the only electrode which pick the signal from the subjects body. BUT HOW ?
  14. 14. WE HAVE TOUNDERSTANDELECTRODE JELLYINTERFACE 1ST
  15. 15. REDOXREACTION?
  16. 16. Electrode – Electrolyte Interface Electrode Electrolyte (neutral charge) C C+, A- in solution Current flow C C+ e- C A- C+ e- A- C+ : Cation A- : Anion e- : electron Fairly common electrode materials: Pt, Carbon, …, Au, Ag,… Electrode metal is use in conjunction with salt, e.g. Ag-AgCl, Pt-Pt black, or polymer coats (e.g. Nafion, to improve selectivity)
  17. 17. Electrode – Electrolyte Interface General Ionic Equations a) n C C ne b) m A A me a) If electrode has same material as cation, then this material gets oxidized and enters the electrolyte as a cation and electrons remain at the electrode and flow in the external circuit. b) If anion can be oxidized at the electrode to form a neutral atom, one or two electrons are given to the electrode. The dominating reaction can be inferred from the following : Current flow from electrode to electrolyte : Oxidation (Loss of e-) Current flow from electrolyte to electrode : Reduction (Gain of e-)
  18. 18. Half Cell Potential A characteristic potential difference established by the electrode and its surrounding electrolyte which depends on the metal, concentration of ions in solution and temperature (and some second order factors) . Half cell potential cannot be measured without a second electrode. The half cell potential of the standard hydrogen electrode has been arbitrarily set to zero. Other half cell potentials are expressed as a potential difference with this electrode.Reason for Half Cell Potential : Charge Separation at InterfaceOxidation or reduction reactions at the electrode-electrolyteinterface lead to a double-charge layer, similar to that which existsalong electrically active biological cell membranes.
  19. 19. Measuring Half Cell PotentialNote: Electrode material is metal + salt or polymer selective membrane
  20. 20. CONFUSE?D? ???
  21. 21. Biopotential Electrodes – The Basics  The interface between the body and electronic measuring devices  Conduct current across the interface  Current is carried in the body by ions  Current is carried in electronics by electrons  Electrodes must change ionic current into electronic current  This is all mediated at what is called the Electrode- Electrolyte Interface or the Electrode-Tissue Interface
  22. 22. Electrode skin jelly interface ELECTROLYTETO -SKIN TISSUEINSTRUMENT METAL- INTERFACE ELECTROLYTE ELECTROLYTE INTERFACE
  23. 23. Current Flow at the Electrode-Electrolyte Interface  Ion- flow  Electrons move in opposite Electron flow direction to current flow Ion+ flow   Cations (C+ ) move in same direction as current flow  Anions (A– ) move in opposite direction of current flow  Chemical oxidation (current flow right) - reduction (current flow left) reactions at the interface: + Current flow  C C++e– (5.1) The current crosses it from left to right. A– A + e– (5.2) The electrode consists of metallic atoms C. The electrolyte is an aqueous solution  No current at equilibrium containing cations of the electrode metal C+ and anions A-.
  24. 24. Half-Cell Potential When metal (C) contacts electrolyte, oxidation (C  C + + e – ) or reduction (A-  A + e –) begins immediately. Local concentration of cations at the surface changes. Charge builds up in the regions. Electrolyte surrounding the metal assumes a different electric potential from the rest of the solution. This potential difference is called the half-cell potential ( E0 ). Separation of charge at the electrode-electrolyte interface results in a electric double layer (bilayer). Measuring the half-cell potential requires the use of a second reference electrode. By convention, the hydrogen electrode is chosen as the reference.
  25. 25. Standard Hydrogen electrodeNote: Ag-AgCl has low junctionpotential & it is also very stable ->hence used in ECG electrodes!
  26. 26. Nernst Equation When two aqueous ionic solutions of different concentration are separated by an ion-selective semi-permeable membrane, an electric potential exists across the membrane. For the general oxidation-reduction reaction Note: interested in A B C D ne ionic activity at the electrode The Nernst equation for half cell potential is (but note temp dependence RT a a E E0 ln C D nF a A aB where E0 : Standard Half Cell Potential E : Half Cell Potential a : Ionic Activity (generally same as concentration) n : Number of valence electrons involved
  27. 27. Equivalent Circuit Cd : capacitance of electrode-eletrolyte interface Rd : resistance of electrode-eletrolyte interface Rs : resistance of electrode lead wire Ecell : cell potential for electrode Corner frequency Rd+Rs Rs Frequency Response
  28. 28. Electrode Skin Interface Ehe Alter skin transport Electrode Cd Rd (or deliver drugs) by: Sweat glands Gel Rs and ducts Pores100 produced Ese EP by laser, ultrasound Stratum Corneum Epidermis Ce Re CP RP or by iontophores100 is Dermis and subcutaneous layer Ru Nerve Skin impedance for 1cm2 patch: endings Capillary 200kΩ @1Hz 200 Ω @ 1MHz
  29. 29. Polarization If there is a current between the electrode and electrolyte, the observed half cell potential is often altered due to polarization. Overpotential Difference between observed and zero-current half cell potentials Activation Resistance Concentration The activation energyCurrent changes resistance Changes in distribution barrier depends on the of electrolyte and thus, of ions at the electrode- direction of current and a voltage drop results. electrolyte interface determines kinetics V p VR VC VA Note: Polarization and impedance of the electrode are two of the most important electrode properties to consider.
  30. 30. Polarizable and Non-Polarizable Electrodes Use for recording Perfectly Polarizable Electrodes These are electrodes in which no actual charge crosses the electrode- electrolyte interface when a current is applied. The current across the interface is a displacement current and the electrode behaves like a capacitor. Example : Ag/AgCl Electrode Perfectly Non-Polarizable Electrode These are electrodes where current passes freely across the electrode- electrolyte interface, requiring no energy to make the transition. These electrodes see no overpotentials. Example : Platinum electrode Use for stimulationExample: Ag-AgCl is used in recording while Pt is use instimulation
  31. 31. Motion Artifact Why When the electrode moves with respect to the electrolyte, the distribution of the double layer of charge on polarizable electrode interface changes. This changes the half cell potential temporarily. What If a pair of electrodes is in an electrolyte and one moves with respect to the other, a potential difference appears across the electrodes known as the motion artifact. This is a source of noise and interference in biopotential measurementsMotion artifact is minimal for non-polarizable electrodes
  32. 32. But one thing that should notbe stopped is questioning
  33. 33. E-mail: techyadav@gmail.com, lokendra@technovast.co.inM: +91-9694958872,09868732573
  34. 34. THANK YOU

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