Identification of suitable substrate for oyster mushroom cultivation

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Identification of suitable substrate for oyster mushroom cultivation

  1. 1. Identification of suitable and efficient substrate for theproduction of Oyster Mushrooms (Pleurotus ostreatus) By:T-Gayathiri
  2. 2. Contents Introduction Objectives Materials and Methods Results and Discussion Conclusions Suggestions References
  3. 3. Introduction
  4. 4.  Saprophytic fungi - Basidiomycetes & Ascomycetes. Mushrooms cultivation - year 600, Asia. Biggest manufacturer - China 70%. Medicinal properties: Nutritional properties: Oyster mushroom Pleurotus ostreatus
  5. 5. Pinheads Button stage Development stages(Pleurotus ostreatus) Full bloom
  6. 6. Objectives
  7. 7.  To find out the suitability of Pleurotus ostreatus cultivation in Batticaloa district To find out the suitable substrate for Pleurotus ostreatus cultivation To find out the substrate with efficient production To find out the substrate which takes shortest period
  8. 8. Materials andMethods
  9. 9.  Location Duration Experiment
  10. 10. Treatments and compositionTreatments CompositionT1 SawdustT2 Paddy strawT3 Dry leavesT4 Shredded paperT5 Sawdust + Paddy straw (1:1)T6 Sawdust + Dry leaves (1:1)T7 Sawdust + Shredded paper (1:1)
  11. 11. Chopped Heat NutrientRaw materials treatment supplementInoculation Autoclaving Filling substrate bagsIncubation Growing period period
  12. 12.  Constituents:  Substrate  Rice bran 10%  CaCO3 2%  Soya bean flour 1%  Epsum salt (MgSO4) 0.2% Growth conditions:  Temperature range 15-30 o C  Optimum growth for mycelium 25- 28 o C  Relative humidity >75
  13. 13. Evaluation was done for, Average yield Total numbers of blooms Large bloom percentage Harvest interval Large bloom diameter Large bloom weight Small bloom diameter Small bloom weight
  14. 14. Data analysis ANOVA DMRT
  15. 15. Results andDiscussion
  16. 16. The average yield (fresh weight) of oyster mushrooms in different substrates 60 a*Average yield (g / harvest ) ab 50 40 abc bc 30 c c Average yield c 20 10 0 T1 T2 T3 T4 T5 T6 T7 Treatments
  17. 17. T1 Average yield T2 from different treatmentsT3 T4T5 T6 T7
  18. 18. The total numbers of blooms produced from different substrates 14 a* aTotal number of blooms / 12 a 10 ab ab harvest 8 ab Total number of blooms 6 b 4 2 0 T1 T2 T3 T4 T5 T6 T7 Treatments
  19. 19. The percentage of large blooms produced from different substratesTreatment Large bloom percentage T1 31.551 2.4528 a* T2 24.9863 16.4311 a T3 31.2607 6.8495 a T4 27.1925 7.6683 a T5 36.6527 23.9670 a T6 27.1083 7.1975 a T7 48.254 6.6040 a
  20. 20. The harvest interval of oyster mushrooms produced from different substrates 7 a* 6Harvest interval (days) ab 5 ab ab 4 ab b Harvest interval 3 b 2 1 0 T1 T2 T3 T4 T5 T6 T7 Treatments
  21. 21. The efficient substrate for the production of oyster mushrooms 60Average yield(g)/Total number interval(days)/Large bloom 50 Average yield of blooms/Harvest percentage(%) 40 Total number of blooms 30 Harvest interval 20 Large bloom 10 percentage 0 T1 T2 T3 T4 T5 T6 T7 Treatments
  22. 22. Size and weight of the mushroom bloomsTreatment Large bloom Large bloom Small bloom Small bloom diameter weight diameter weightT1 6.1458 + 0.0955 5.765 + 0.4276 1.9792 + 0.0722 1.0225 + 0.1607T2 7.3958 + 1.2060 8.98 + 2.8102 2.1667 + 0.7324 1 + 0.9855T3 7.0625 + 1.2640 7.6417 +2.2169 1.9792 + 0.3442 0.795 + 0.1506T4 7.3125 + 0.1654 8.8905+ 1.3436 2.2708 + 0.2526 1.2771 + 0.1732T5 8.0417 + 1.0777 12.4668 +2.7906 2.875 + 0.8197 3.1189 + 2.9978T6 6.0417 + 0.8356 5.0786+ 0.6109 2.7292 + 0.3662 1.3251 + 0.2481T7 10.3325 + 0.5 22.9867+ 1.8194 3.7083 + 2.1518 5.4178 + 7.0960
  23. 23. Mushroom fly Black mould Abnormal blooms
  24. 24. Conclusions
  25. 25.  Cultivation of oyster mushrooms (Pleurotus ostreatus) is suitable in Batticaloa district. The sawdust + paddy straw and sawdust + shredded paper substrate are the suitable and efficient substrates. The sawdust + paddy straw is the substrate with efficient production. The sawdust + paddy straw substrate took the shortest time for production.
  26. 26. Suggestions
  27. 27.  Further studies should be carried out by using the substrate after the fermentation of the raw materials for 1-2 days. The studies should be needed by using other materials such as maize leaves, corn cobs, sugarcane leaves, rice bran …etc. The studies should be needed to find out the effect of various grain spawns.
  28. 28. References
  29. 29.  Bhal,N(2000).Hand book on mushrooms,4th Ed. Oxford and IBH publishing Co.Pvt.Ltd, New Delhi, India.pp.99. Amuthan,G.,Visvanathan,R.,Kailappan,R. and Sreenarayanan,V.V.(1999).Studying of osmo-air drying of milky mushroom.Calocyba indica.Mushroom Res.8:49-52. Chang,S.T. and P.G.Miles (1987).Edible mushrooms and their cultivation.CBS publishers and distributors,Delhi,India,pp.3,8,12,225-251. Pathak,V.N.,N.Yadav and M.Gaur (1998).Mushroom production and processing technology.Agrobios,India.pp. 1, 76-82.

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