Photosynthesis 6 light independent reaction

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Photosynthesis 6 light independent reaction

  1. 1. Cyclic and Non-cyclic photophosphorylation http://highered.mcgraw- hill.com/sites/9834092339/student_view0/chapter8/cyclic_and_noncyclic_photophosp horylation.html
  2. 2. Light independent reaction (k) outline how the products of the light dependent stage are used in the light independent stage (Calvin cycle) to produce triose phosphate (TP) (reference should be made to ribulose bisphosphate (RuBP), ribulose bisphosphate carboxylase (rubisco) and glycerate 3-phosphate (GP), but no other biochemical detail is required); (l) explain the role of carbon dioxide in the light-independent stage (Calvin cycle); aka Dark Reaction aka Calvin Cycle aka Carbon Fixation Reaction
  3. 3. Photosynthetic electron-transfer reactions in thylakoid membrane ATP NADPH Carbon-fixation reactions in stroma Sugars, amino acids and fatty acids Water Carbon dioxide Oxygen + Chloroplast Cytosol LDR LIR
  4. 4. Word ladder • Toad • ---- • ---- • ---- • ---- • ---- • ---- • ---- • ---- • Frog • Toad • (street) • (leaf through) • (toward the back) • (phobia) • (achievement) • (level or smooth) • (banner) • (whip) • Frog • Toad • road • read • rear • fear • feat • flat • flag • flog • Frog
  5. 5. Phase one – carbon fixation Ribulose bisphosphate (RuBP) (aka ribulose biphosphate) (aka ribulose 1,5 bisphosphate) Ribulose bisphosphate carboxylase (RuBisCo) Glycerate 3- phosphate (GP) (aka 3-phosphoglycerate) 2X
  6. 6. Phase two – reduction (part one) Glycerate 3- phosphate (GP) (aka 3-phosphoglycerate) 1,3- bisphosphoglycerate (GP)
  7. 7. Triose Phosphate (TP) (aka glyceraldehyde-3-phosphate) (aka G3P) Phase two – reduction (part two) 1,3- bisphosphoglycerate (GP)
  8. 8. Regeneration Several steps Triose Phosphate (TP) (aka glyceraldehyde-3-phosphate) (aka G3P) Ribulose 5-phosphate
  9. 9. Return to the start Ribulose bisphosphate (RuBP) (aka ribulose biphosphate) (aka ribulose 1,5 bisphosphate) Ribulose 5-phosphate
  10. 10. Glycerate 3-phosphate 2. Reduction 1. Carbon fixation 6x Triose phosphate 1x Triose phosphate 5x Triose phosphate 3. RuBP is regenerated from triose phosphate Calvin Cycle/LIR Glucose
  11. 11. The role of Carbon Dioxide
  12. 12. Useful Animation • http://www.science.smith.edu/departments/ Biology/Bio231/calvin.html • http://highered.mcgraw- hill.com/sites/007337797x/student_view0/ch apter7/animation_quiz_- _how_the_calvin_cycle_works.html
  13. 13. Summary test… • The light independent reaction is also known as the (1) cycle. In the process, carbon dioxide combines with a 5- carbon compound called (2) to form a 6- carbon intermediate that immediately splits into 2 molecules of (3). By the addition of (4) and (5) formed in the (6) reaction, each of these molecules is converted into a (7) molecule. These combine to form a (8) sugar that can then be made into starch by a process called (9).
  14. 14. Glycerate 3-phosphate 2. Reduction 1. Carbon fixation 6x Triose phosphate 1x Triose phosphate 5x Triose phosphate 3. RuBP is regenerated from triose phosphate Calvin Cycle/LIR Glucose Can be used to make carbohydrates, lipids or amino acids

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