Actins are a group of proteins that play a role in different types of cell mortality. They are universally expressed in eukaryotic cells. Beta actin is one of six isoforms of actin that exist in human cells. It is also one of the two non-muscle cytoskeletal actins. The β-actin antibody detects endogenous levels of β-actin protein. Anti-β-actin-http://www.stjohnslabs.com/b-actin-antibody-p-98576 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“No background detected. The antibody was highly visible in the sample. Highly specific.
In Normal NPCs and TRF2-knockdwon NPCs cells, there were target bands detected by STJ96941 Anti-β-
actin antibody (Figure: Lane1: TRF2 control NPCs; Lane 2: TRF2 knockdown NPCs).“- Sitti, Imperial College
London, United Kingdom.
Antibody Customer Review:
STJ96941 Anti-β-actin antibody
Antibody Specificity:
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Protocol
Treatment of materials: When cells were cultured to 70%-80% plating density, RIPA lysis buffer with 1:100 protease
inhibitors (Sigma) and PMSF were added. 5X loading buffer were added prior to running. No heating.
E.g. Gel electrophoresis: 12% polyacrylamide gel, constant voltage 120 V, 90 min.
Transfer: 0.45 nm PVDF membrane, wet transfer, constant voltage 20 V, 90 min.
Blocking: 1X TBST with 5% skimmed milk powder for 1 hr in room temperature.
Primary antibody probing: primary antibody was added to the solution of 1X TBST with 5% skimmed milk powder,
4˚C on roller for overnight.
Membrane wash: 1X TBST wash for 3 times at 10 minutes intervals.
Secondary antibody probing: secondary antibody was added to the solution of 1X TBST with 5% skimmed milk
powder with 1:5000 dilution ratio, for 1 hr
Membrane wash: 1X TBST wash for 3 times at 10 minutes intervals.
Visualization: ECL visualization for 1 sec.
Cell Line: Human Neural Progenitor Cells
Method of validation: Western Blot
Primary Antibody: STJ96941 Anti-β-actin antibody
Secondary Antibody
HRP goat anti-mouse, light
chain specific
Dilution ratio: 1:1000
Gel electrophoresis information：12% polyacrylamide gel, constant voltage 120 V, 90 min.
Transfer information：0.45 nm PVDF membrane, semi-dry transfer, constant voltage 20 V, 90 min.
Lane No. Antigen
Loading
amount
Primary
antibody
Primary
antibody
dilution ratio
Secondary
antibody dilution
ratio
Target band KD
Visualizati
on time
1
hESC-derived
NPCs
20ug
STJ96941
Anti-β-actin
antibody
1:1000 1:5000 ~45KD
1S
2
TRF2-
knockdown
NPCs
1S