Isolating DNA from a banana…yes a banana!!!
 
How do we take out the DNA? <ul><li>Detergent (shampoo)  </li></ul><ul><li>Breaks down the cell membrane </li></ul><ul><li...
Filter <ul><li>2. Filter the banana “goo” with a coffee filter. </li></ul><ul><li>-This removes everything that you do not...
Now we remove the DNA <ul><li>Add very cold rubbing alcohol </li></ul><ul><li>This will allow the DNA to  </li></ul><ul><l...
Instructions and Measurements <ul><li>Materials:  (per person) </li></ul><ul><li>piece fruit (1/4 of a banana) </li></ul><...
First Step <ul><li>Take 1/4 of a banana and place it in a bowl. </li></ul><ul><li>Add four tablespoons of water, 1 tablesp...
Second Step <ul><li>Take six tablespoons of this fruit puree and add it to the filter. </li></ul><ul><li>Make sure the fil...
Third Step <ul><li>Once you have your filtrate add 3 tablespoons of very cold rubbing alcohol. </li></ul><ul><li>Let it st...
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Isolating dna from a banana

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Isolating dna from a banana

  1. 1. Isolating DNA from a banana…yes a banana!!!
  2. 3. How do we take out the DNA? <ul><li>Detergent (shampoo) </li></ul><ul><li>Breaks down the cell membrane </li></ul><ul><li>and nuclear membrane. </li></ul>
  3. 4. Filter <ul><li>2. Filter the banana “goo” with a coffee filter. </li></ul><ul><li>-This removes everything that you do not need. </li></ul><ul><li>-Take the filtrate </li></ul>Banana goo What you need!!!
  4. 5. Now we remove the DNA <ul><li>Add very cold rubbing alcohol </li></ul><ul><li>This will allow the DNA to </li></ul><ul><li>precipitate. </li></ul><ul><li>Now you may remove the </li></ul><ul><li>DNA with a toothpick. </li></ul>
  5. 6. Instructions and Measurements <ul><li>Materials: (per person) </li></ul><ul><li>piece fruit (1/4 of a banana) </li></ul><ul><li>a coffee filter </li></ul><ul><li>two normal sized plastic cups </li></ul><ul><li>15 ml of rubbing alcohol (prior to activity place test tubes with alcohol in freezer) </li></ul><ul><li>a teaspoon of salt </li></ul><ul><li>4 teaspoons of water </li></ul><ul><li>1 Tablespoon clear shampoo </li></ul><ul><li>1 toothpick </li></ul>
  6. 7. First Step <ul><li>Take 1/4 of a banana and place it in a bowl. </li></ul><ul><li>Add four tablespoons of water, 1 tablespoon of shampoo and 1 teaspoon of salt. </li></ul><ul><li>Start mashing the banana for until it comes to the consistency of a smoothie (five to ten minutes) </li></ul>
  7. 8. Second Step <ul><li>Take six tablespoons of this fruit puree and add it to the filter. </li></ul><ul><li>Make sure the filter sits properly in the cup. You do not want the filter to fall (You may need to hold it) </li></ul>
  8. 9. Third Step <ul><li>Once you have your filtrate add 3 tablespoons of very cold rubbing alcohol. </li></ul><ul><li>Let it stand for a minute DO NOT shake it. </li></ul><ul><li>You will then see a white “glob”, this is DNA!!! </li></ul><ul><li>Take your toothpick and extract the DNA. </li></ul>

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