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Isolating dna from a banana
 

Isolating dna from a banana

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    Isolating dna from a banana Isolating dna from a banana Presentation Transcript

    • Isolating DNA from a banana…yes a banana!!!
    •  
    • How do we take out the DNA?
      • Detergent (shampoo)
      • Breaks down the cell membrane
      • and nuclear membrane.
    • Filter
      • 2. Filter the banana “goo” with a coffee filter.
      • -This removes everything that you do not need.
      • -Take the filtrate
      Banana goo What you need!!!
    • Now we remove the DNA
      • Add very cold rubbing alcohol
      • This will allow the DNA to
      • precipitate.
      • Now you may remove the
      • DNA with a toothpick.
    • Instructions and Measurements
      • Materials: (per person)
      • piece fruit (1/4 of a banana)
      • a coffee filter
      • two normal sized plastic cups
      • 15 ml of rubbing alcohol (prior to activity place test tubes with alcohol in freezer)
      • a teaspoon of salt
      • 4 teaspoons of water
      • 1 Tablespoon clear shampoo
      • 1 toothpick
    • First Step
      • Take 1/4 of a banana and place it in a bowl.
      • Add four tablespoons of water, 1 tablespoon of shampoo and 1 teaspoon of salt.
      • Start mashing the banana for until it comes to the consistency of a smoothie (five to ten minutes)
    • Second Step
      • Take six tablespoons of this fruit puree and add it to the filter.
      • Make sure the filter sits properly in the cup. You do not want the filter to fall (You may need to hold it)
    • Third Step
      • Once you have your filtrate add 3 tablespoons of very cold rubbing alcohol.
      • Let it stand for a minute DO NOT shake it.
      • You will then see a white “glob”, this is DNA!!!
      • Take your toothpick and extract the DNA.