Anatomy of Some Dicot Plants in Salaya  Putthamonthol Nakornpathom Mahidol Wittayanusorn School, Thailand
Introduction
 
Introduction Each kind of vegetables has its own specific  nutrition and benefit. The vegetables under our study  are Chil...
Introduction   Since all the study in anatomy of plants are always on the   basis of tissue of these plants are not widely...
Materials, Chemicals and Equipments
Materials <ul><li>Slide   </li></ul><ul><li>Cover glass   </li></ul><ul><li>Aluminium Foil  </li></ul><ul><li>Paper </li><...
Chemicals <ul><li>Paraffin   8.  TBA (Tertiary Butyl Alcohol) </li></ul><ul><li>Paraffin oil   9.  Absolute ethyl alcohol ...
Equipments <ul><li>Microtome    9.  Microscope </li></ul><ul><li>Razor blade    10.  Oven </li></ul><ul><li>Vacuum pump  1...
Methods
Method Paraffin method Selecting Fixing Dehydration Infiltration embedding Sectioning Affixation Staining Mounting
Select and cut plant into square pieces, 0.5 cm in length.
Preserve the specimens in   Formalin Acetic Acid (FAA) at least 8 Hr.
Suck the air out of the specimens   with vacuum pump until there isn’t any bubble.
Wash them with   ethanol 50%, at least 3 Hr.   3 times.
Steep in   TBA 50% 70% 85% 95% 100% At least 12 Hr. 50% 70% 85% 95% 100%
Steep in   pure TBA at least 12 Hr.   3 times.
Steep in   TBA   mixed with paraffin oil 1:1 at least 12 Hr.   at 60 Celsius degrees. 60 o
Steep in   paraplast at least 12 Hr.   at 60 Celsius degrees. In   3   times. 60 o
Eliminate bubble while Embed in   paraplast   and wait until getting cool.
Cut in shape of cubic and stick on wooden block.   Then cut with   rotary microtome.
Mount them with   Formalin 3%   and haupt’s adhesive and lay on   slide warmer at 40 -50 o C. Then keep at room temperatur...
Dip in   xylene 5 Minutes Xylene+ethanol  ether +   ethanol  Ethanol 95%  Ethanol 70%   Ethanol 50%   Ethanol 30%
Steep in   Safranin-O 1   Hr.   Then wash with water.
Ethanol 95%   Ethanol 70%   Ethanol 50%   Dip in   Ethanol 30%  5 Minutes
Absolute Ethanol +Xylene Absolute Ethanol Wash with Ethanol 95% Clove oil Drop   fast green  Steep in   Xylene 6   Hr.
Cover them with cover glass by   Canada Balsam or   permount. Then bake them in oven.
Clean up the surplus mountant  and keep them in the slide box.
Examine with microscope and analysis the result.
Methods Search for information Select and preserve the plant specimens in FAA  Dehydrate the tissue by preserving it in Te...
Methods Preserve the tissue in melting paraplast at 60 o C Embedding Cut it with rotary microtome Stretch the specimens by...
Methods Staining with Safrinin O and Fast green Wash the stain out Mounting with mounting medium (Permount), Drop it and c...
Results & Discussion
Results Parsley Leaf 40x
Results Dill Leaf 100x
Results Celery Leaf 40x
Results Chilli Fruit 40x
Results Bitter Cucumber Fruit 40x
Results Bitter Cucumber Fruit 40x
Results Bitter Cucumber Fruit 40x
 
Conclusion
Plants which people admire to eat their leafs, celery had larger area of dermal tissue than dill and celery, respectively....
References Consortium of Thai Medical Schools.  1997.  Local Plant.   The War Veterans  Organization Press, Bangkok C. Bri...
References Prof. Ass. Dr. Prasart Keurmanee.  2006.  Plant  Tissue Technique.   Botany Department,  Science  Faculty, Kase...
References Sylvia Lee-Huang, Paul L. Huang, Philip L. Huang, Aldar S.  Bourinbaiar, Hao-Chia  Chen and Hsiang-Fu  Kung.  1...
References Tiamjai Komkris.  2003.  Plant Anatomy.  Botany  Department, Science Faculty, Kasetsart  University, Bangkok Ur...
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Anatomy of Some Dicot Plant in Salaya Putthamonthon Nakhonpathom

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Anatomy of Some Dicot Plant in Salaya Putthamonthon Nakhonpathom

  1. 1. Anatomy of Some Dicot Plants in Salaya Putthamonthol Nakornpathom Mahidol Wittayanusorn School, Thailand
  2. 2. Introduction
  3. 4. Introduction Each kind of vegetables has its own specific nutrition and benefit. The vegetables under our study are Chilli ( Capsicum frutescens L. var. frutescens ) , Celery ( Apium graveolens L.) , Parsley ( Erygium foetidum Linn. ) , Dill ( Anethum gaveolens L.) and Bitter Cucumber ( Momordica charantia L.) . All of which are abundant and easy to obtain in the Salaya area and, moreover, the study in the level of tissue of these plants are not widely known
  4. 5. Introduction Since all the study in anatomy of plants are always on the basis of tissue of these plants are not widely known. Since all the study in anatomy of plants are always on the basis of tissue level study, the data of this project can be used as the basis of other kinds of studies regarding vegetables, such as the study of the relation between the plants’ structure and the ability to collect antioxidant substance.
  5. 6. Materials, Chemicals and Equipments
  6. 7. Materials <ul><li>Slide </li></ul><ul><li>Cover glass </li></ul><ul><li>Aluminium Foil </li></ul><ul><li>Paper </li></ul>
  7. 8. Chemicals <ul><li>Paraffin 8. TBA (Tertiary Butyl Alcohol) </li></ul><ul><li>Paraffin oil 9. Absolute ethyl alcohol </li></ul><ul><li>Xylene 10. FAA 50% </li></ul><ul><li>95% Ethanol (Formalin aceto-alcohol) </li></ul><ul><li>Fast green 11. Haupt’s adhesive </li></ul><ul><li>Safranin O 12. Distilled water </li></ul><ul><li>Glycerine 13. Ether </li></ul>
  8. 9. Equipments <ul><li>Microtome 9. Microscope </li></ul><ul><li>Razor blade 10. Oven </li></ul><ul><li>Vacuum pump 11. Slide warmer </li></ul><ul><li>Alcohol lamp 12. Camera </li></ul><ul><li>Thermometer 13. Beaker </li></ul><ul><li>Paintbrush 14. Staining Jar </li></ul><ul><li>Dropper 15. Plate </li></ul><ul><li>Pin 16. Forceps </li></ul>
  9. 10. Methods
  10. 11. Method Paraffin method Selecting Fixing Dehydration Infiltration embedding Sectioning Affixation Staining Mounting
  11. 12. Select and cut plant into square pieces, 0.5 cm in length.
  12. 13. Preserve the specimens in Formalin Acetic Acid (FAA) at least 8 Hr.
  13. 14. Suck the air out of the specimens with vacuum pump until there isn’t any bubble.
  14. 15. Wash them with ethanol 50%, at least 3 Hr. 3 times.
  15. 16. Steep in TBA 50% 70% 85% 95% 100% At least 12 Hr. 50% 70% 85% 95% 100%
  16. 17. Steep in pure TBA at least 12 Hr. 3 times.
  17. 18. Steep in TBA mixed with paraffin oil 1:1 at least 12 Hr. at 60 Celsius degrees. 60 o
  18. 19. Steep in paraplast at least 12 Hr. at 60 Celsius degrees. In 3 times. 60 o
  19. 20. Eliminate bubble while Embed in paraplast and wait until getting cool.
  20. 21. Cut in shape of cubic and stick on wooden block. Then cut with rotary microtome.
  21. 22. Mount them with Formalin 3% and haupt’s adhesive and lay on slide warmer at 40 -50 o C. Then keep at room temperature for 5 days.
  22. 23. Dip in xylene 5 Minutes Xylene+ethanol ether + ethanol Ethanol 95% Ethanol 70% Ethanol 50% Ethanol 30%
  23. 24. Steep in Safranin-O 1 Hr. Then wash with water.
  24. 25. Ethanol 95% Ethanol 70% Ethanol 50% Dip in Ethanol 30% 5 Minutes
  25. 26. Absolute Ethanol +Xylene Absolute Ethanol Wash with Ethanol 95% Clove oil Drop fast green Steep in Xylene 6 Hr.
  26. 27. Cover them with cover glass by Canada Balsam or permount. Then bake them in oven.
  27. 28. Clean up the surplus mountant and keep them in the slide box.
  28. 29. Examine with microscope and analysis the result.
  29. 30. Methods Search for information Select and preserve the plant specimens in FAA Dehydrate the tissue by preserving it in Tertiary Butyl Alcohol (TBA)
  30. 31. Methods Preserve the tissue in melting paraplast at 60 o C Embedding Cut it with rotary microtome Stretch the specimens by using 3% formalin and Haupt’s decisive
  31. 32. Methods Staining with Safrinin O and Fast green Wash the stain out Mounting with mounting medium (Permount), Drop it and cover it with cover glass
  32. 33. Results & Discussion
  33. 34. Results Parsley Leaf 40x
  34. 35. Results Dill Leaf 100x
  35. 36. Results Celery Leaf 40x
  36. 37. Results Chilli Fruit 40x
  37. 38. Results Bitter Cucumber Fruit 40x
  38. 39. Results Bitter Cucumber Fruit 40x
  39. 40. Results Bitter Cucumber Fruit 40x
  40. 42. Conclusion
  41. 43. Plants which people admire to eat their leafs, celery had larger area of dermal tissue than dill and celery, respectively. While the ratio of ground tissue area and vascular tissue area, celery had much value than celery and dill, respectively. In another group that people admire to eat their fruits, chili had larger area of dermal tissue than bitter cucumber. But bitter cucumber had more value of ratio of ground tissue area and vascular tissue area than chili.
  42. 44. References Consortium of Thai Medical Schools. 1997. Local Plant. The War Veterans Organization Press, Bangkok C. Brickell. 2003. The Royal Horticultural Society A-Z of GARDEN PLANT volume1: A-J. Dorling Kindersley L imited, London. L. Bremness. 1994. HERBS. Dorling Kindersley Limited, London. L. Bremness. 2000. HERBS. Dorling Kindersley Limited, London. Faculty, Kasetsart University, Bangkok
  43. 45. References Prof. Ass. Dr. Prasart Keurmanee. 2006. Plant Tissue Technique. Botany Department, Science Faculty, Kasetsart University, Bangkok R.F. Evert. 2006. Esau’s Plant Anatomy 3rd edition. John Wiley & Sons, Inc., New Jersey. Rost T . L . , M . G . Barbour, C . R . Stocking, and T . R . Murphy . Plant Biology . 2nd edition . Thomson Higher Education, USA .
  44. 46. References Sylvia Lee-Huang, Paul L. Huang, Philip L. Huang, Aldar S. Bourinbaiar, Hao-Chia Chen and Hsiang-Fu Kung. 1995. Inhibition of The Integrase of Human Immunodeficiency Virus (HIV) Type 1 by Anti-HIV Plant Proteins MAP30 and GAP31. M.S. thesis, Department of Biochemistry, New York University School of Medicine, New York, NY 10016 ; tAmerican BioSciences, New York, NY 10021 ; INational Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 ; and IlLaboratory of Biochemical Physiology, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD. Faculty, Kasetsart University, Bangkok
  45. 47. References Tiamjai Komkris. 2003. Plant Anatomy. Botany Department, Science Faculty, Kasetsart University, Bangkok Urai Jiramonkholkarn. 2004. Indigenous Vegetables 1. Ammarin Printing and Publishing Ltd., Bangkok Urai Jiramonkholkarn. 2004. Indigenous Vegetables 2. Ammarin Printing and Publishing Ltd., Bangkok
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