test antibiotic on vivo

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test antibiotic on vivo

  1. 1. D-:GROUPProf. Masood-ul-Hassan JavedSaadi Aljundi ID: 11110044Abdulrahman Hameed ID:11110042Ali Abdulghafor ID:11110061Yaser Alhodaithy ID:11110071Mustafa Fouad ID:11110120Hamad Emad Dhuhayr ID:10110067ScientificAssignment5.1Block
  2. 2. ContentsIntroductionMaterial and methodresultDiscuses41235 Conclusion6 References7
  3. 3. ( a pharmacological company which study the development ofantibiotics). facilitated by laboratories and materials for performingpharmacological and microbial antibiotics’ researches having a specialized section for the animal experiments. the company has a research on new antibiotics.To study the effectiveness of an antibiotic by:– Firstly, pharmacological experiment. (vitro)– Secondly, Microbiological experiment. (vitro)– Thirdly, experiment on animal (vivo)– And finally these experiments will enable us to test the drug onhumansINTRODUCTIONANDAIMS
  4. 4. 1) Antibiotic SAAHYM.2) Animal models:- Rabbits.3) criteria:- used 12 male healthy rabbits.4) Housing:- They were put in a big, quiet cage andgermfree room with good ventilation, and were exposed tothe sun light at temperature of 27C°.5) feeding:- They were fed thrice a day, at the earlymorning, the afternoon, and the evening each followed bycollection of urine samples.Material and method
  5. 5. Pharmacological part:-In this part, we test if the drug is metabolized in the liverand if it stable in the stomach or not. to examine metabolism by a liver homogenate. to test stability by used solution of ph= 2. to divide 6 rabbits to 2 groups.Microbiological part:-In this part, we study the bacteria in aerobic culture andthe effect of the antibiotic SAAHYM on them. Sterilization of loop in order to put the sample s to forminoculation and distribute it by “blood agar media”. additionally test were performed such as urease and coagulasetest. measured the sensitivity the drug by “disk diffusion method” Selected 6 male rabbits divided into two groups of threeCon>>Material and method
  6. 6.  Pharmacological Part:--From this table we can observe that the drug is metabolized by theliver and at the same time is stable (not hydrolyzed) in the stomach.- After HPLC analysis of the rabbits’ urine, the experimental groupshowed presence of the drug metabolites in the urine.Result
  7. 7.  Microbiological part:-Finally after doing the experiments we found out that:• SAAHYM is effective because of the widest clear zone aroundthe bacteria• And hence, we can complete the test and apply the drug on Vivo.Result con>>
  8. 8. Pharmacological Part:- before take any new drugs we have to experiment it inanimal. when test it we have to concern on its metabolismand affect the highly acidic on it by stomach. we used liver homogenate to test the metabolism. we used the animals’ urine because, aftermetabolism, the drugs are converted to hydrophilicsubstances so they can be excreted via urine.Discuses
  9. 9. Microbiological part:- (NW 2101) is a new discovered type of bacteria that causes(RBCs hemolysis mediated exotoxin) which is resistant to allknown antibiotics. It’s mechanism of resistance is based onthe presence of the enzymes β-lactamases. The antibiotic SAAHYM’s mechanism of action is throughstoppage of the bacterial cell wall synthesis and stimulatingthe autolytic enzymes present in the cell wall of the bacteria.Discuses con>>
  10. 10.  After the successful results followed this experiment, weneed further research and studies to detect any side effectsthat may result from this new drug, and also to see thepossibility of using and applying this drug in a clinical trial inthe future. After the successful results followed this experiment, weneed further research and studies to detect any side effectsthat may result from this new drug, and also to see thepossibility of using and applying this drug in a clinical trial inthe future.Conclusion
  11. 11.  H. P. Rang, M.M. Dale, J.M. Ritter, R.J. Flower, G. Henderson:Rang and Dale’s Pharmacology. 7th Edition. (Elsevier Inc. 2012).Chapters: 49-50 C.K.Bnniker, R.Ananthanarayan. Textbook of microbiology, 8thedition (Universities Press (India 2009-2010)). Chapters: 4,5,6,66. Richard A. Harvey, Pamela C. Champe: Lippincott’s IllustratedReviews Microbiology. 2nd Edition. ( Lippincott’s Williams &Wiklins 2007). Chapter:19 Lectures, papers, journals about Animal Experiment provided byMaastricht University and Alrajhi Colleges.References
  12. 12. Thank you

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