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BY SREEREMYA.S
Anaerobic environment
Anaerobic environment
 Also microaerophiles like Campylobacter jejuni very 
important in medial terms 
 For proper diagnosis and for studies of anaerobic 
environments culture techniques are desirable
 Culture of anaerobes is extremely difficult due to the 
need to exclude oxygen, slow growth and complex 
growth requirements 
 Molecular methods based on DNA analysis and direct 
microscopy have shown that we are largely ignorant of 
the microbial world and previously unknown diversity 
has been discovered
Culture methods 
 Anaerobes differ in their sensitivity to oxygen and the 
culture methods employed reflect this - some are 
simple and suitable for less sensitive organisms, others 
more complex but necessary for fastidious anaerobes 
 Vessels filled to the top with culture medium can be 
used for organisms not too sensitive
 Most common adaptation of media is the addition of a 
reducing agent, e.g. thioglycollate, cysteine 
 Acts to reduce the oxygen to water, brings down the 
redox potential -300mV or less. 
 Can add a redox indicator such as rezazurin, pink in 
the presence of oyxgen - colourless in its absence
 Deep culture tubes can be used to test whether an 
unknown organism is anaerobic/facultative or aerobic 
 Thioglycollate added to culture medium, oxygen only 
found near top where it can diffuse from air -pattern 
of colony formation characteristic of organisms
REDOX POTENTIAL
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment
Anaerobic environment

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Anaerobic environment

  • 4.  Also microaerophiles like Campylobacter jejuni very important in medial terms  For proper diagnosis and for studies of anaerobic environments culture techniques are desirable
  • 5.  Culture of anaerobes is extremely difficult due to the need to exclude oxygen, slow growth and complex growth requirements  Molecular methods based on DNA analysis and direct microscopy have shown that we are largely ignorant of the microbial world and previously unknown diversity has been discovered
  • 6. Culture methods  Anaerobes differ in their sensitivity to oxygen and the culture methods employed reflect this - some are simple and suitable for less sensitive organisms, others more complex but necessary for fastidious anaerobes  Vessels filled to the top with culture medium can be used for organisms not too sensitive
  • 7.  Most common adaptation of media is the addition of a reducing agent, e.g. thioglycollate, cysteine  Acts to reduce the oxygen to water, brings down the redox potential -300mV or less.  Can add a redox indicator such as rezazurin, pink in the presence of oyxgen - colourless in its absence
  • 8.  Deep culture tubes can be used to test whether an unknown organism is anaerobic/facultative or aerobic  Thioglycollate added to culture medium, oxygen only found near top where it can diffuse from air -pattern of colony formation characteristic of organisms