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Fishing for mechanisms regulating senescence in
sockeye salmon
Steven Roberts
Tom Quinn
Leslie Jensen
Caroline Storer
photo: Foist
Salmon Senescence


• Rapid decline to death


• Alongside other complex physiological process


• Rates of senescence var...
What physiological process regulate senescence?
Rationale


• Better understand how environmental change might effect senescence
  physiology


• Determine other traits t...
Preliminary Investigations
Carlson and Quinn 2007


Preliminary Investigations




        Hansen Creek

           Small tributary of Lake Aleknagik...
Preliminary Investigations




        Hansen Creek
Approach

• Sample fish (brain tissue)


• Select candidate genes


• Characterize gene expression patterns for select genes
Preliminary Investigations
relative gene expression



                           Cortisol axis
Preliminary Investigations
Preliminary Investigations


                           Chaperones
relative gene expression
Preliminary Investigations



                        HSP70
   relative protein
     expression




   *Liver
Preliminary Investigations
 relative gene expression


                            olfaction   repro




                 ...
Summary

• Baseline information on physiological changes between “arriving” and
  senescent salmon


• Cortisol signaling ...
Future direction


• Compare expression in populations with different rates of senescence


• Characterize corresponding p...
Roberts Alaska Sen
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Roberts Alaska Sen

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Transcript of "Roberts Alaska Sen"

  1. 1. Fishing for mechanisms regulating senescence in sockeye salmon Steven Roberts Tom Quinn Leslie Jensen Caroline Storer
  2. 2. photo: Foist
  3. 3. Salmon Senescence • Rapid decline to death • Alongside other complex physiological process • Rates of senescence vary within population and across populations • Arrival date to spawning ground correlates with rate of senescence (Hendry et al 2003) • Bear predation plays a role in driving rates (Carlson et al 2007)
  4. 4. What physiological process regulate senescence?
  5. 5. Rationale • Better understand how environmental change might effect senescence physiology • Determine other traits that could be impacted by selective pressures • Model system for studying aging in other taxa
  6. 6. Preliminary Investigations
  7. 7. Carlson and Quinn 2007 Preliminary Investigations Hansen Creek Small tributary of Lake Aleknagik Nearly uniform top to bottom: 4m wide & 10cm deep riffles Annual spawning population of 3,000 – 20,000 sockeye Stream life annually averages below 10 days (usually 7-14)
  8. 8. Preliminary Investigations Hansen Creek
  9. 9. Approach • Sample fish (brain tissue) • Select candidate genes • Characterize gene expression patterns for select genes
  10. 10. Preliminary Investigations relative gene expression Cortisol axis
  11. 11. Preliminary Investigations
  12. 12. Preliminary Investigations Chaperones relative gene expression
  13. 13. Preliminary Investigations HSP70 relative protein expression *Liver
  14. 14. Preliminary Investigations relative gene expression olfaction repro learning immune aging
  15. 15. Summary • Baseline information on physiological changes between “arriving” and senescent salmon • Cortisol signaling declines temporally • Molecular chaperone protein (HSP) is upregulated at senescence • Molecules controlling “other” primary physiological process are differentially expressed • Receptor (NMDA) associated with aging and memory in other taxa declines in senescence
  16. 16. Future direction • Compare expression in populations with different rates of senescence • Characterize corresponding protein expression levels • Take a non-biased approach to identify differences (NGS, Differential Display) • Explore genetic (SNP) and epigenetic (DNA methylation pattern) differences
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