Roberts Alaska Sen
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Roberts Alaska Sen

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Roberts Alaska Sen Roberts Alaska Sen Presentation Transcript

  • Fishing for mechanisms regulating senescence in sockeye salmon Steven Roberts Tom Quinn Leslie Jensen Caroline Storer
  • photo: Foist
  • Salmon Senescence • Rapid decline to death • Alongside other complex physiological process • Rates of senescence vary within population and across populations • Arrival date to spawning ground correlates with rate of senescence (Hendry et al 2003) • Bear predation plays a role in driving rates (Carlson et al 2007)
  • What physiological process regulate senescence?
  • Rationale • Better understand how environmental change might effect senescence physiology • Determine other traits that could be impacted by selective pressures • Model system for studying aging in other taxa
  • Preliminary Investigations
  • Carlson and Quinn 2007 Preliminary Investigations Hansen Creek Small tributary of Lake Aleknagik Nearly uniform top to bottom: 4m wide & 10cm deep riffles Annual spawning population of 3,000 – 20,000 sockeye Stream life annually averages below 10 days (usually 7-14)
  • Preliminary Investigations Hansen Creek
  • Approach • Sample fish (brain tissue) • Select candidate genes • Characterize gene expression patterns for select genes
  • Preliminary Investigations relative gene expression Cortisol axis
  • Preliminary Investigations
  • Preliminary Investigations Chaperones relative gene expression
  • Preliminary Investigations HSP70 relative protein expression *Liver
  • Preliminary Investigations relative gene expression olfaction repro learning immune aging
  • Summary • Baseline information on physiological changes between “arriving” and senescent salmon • Cortisol signaling declines temporally • Molecular chaperone protein (HSP) is upregulated at senescence • Molecules controlling “other” primary physiological process are differentially expressed • Receptor (NMDA) associated with aging and memory in other taxa declines in senescence
  • Future direction • Compare expression in populations with different rates of senescence • Characterize corresponding protein expression levels • Take a non-biased approach to identify differences (NGS, Differential Display) • Explore genetic (SNP) and epigenetic (DNA methylation pattern) differences