Oyster Hemocytes - NSA 2009
Upcoming SlideShare
Loading in...5
×
 

Oyster Hemocytes - NSA 2009

on

  • 1,014 views

 

Statistics

Views

Total Views
1,014
Views on SlideShare
1,014
Embed Views
0

Actions

Likes
0
Downloads
2
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Adobe PDF

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Oyster Hemocytes - NSA 2009 Oyster Hemocytes - NSA 2009 Presentation Transcript

  • Analysis of Genes Isolated from Plated Hemocytes of the Pacific Oyster, Crassostrea gigas Steven Roberts School of Aquatic and Fishery Sciences University of Washington Rick Goetz Great Lakes WATER Institute University of Wisconsin
  • Goal Better understand stress response
  • Immune System Laura Canesi, Gabriella Gallo, Miriam Gavioli, and Carla Pruzzo Bacteria–hemocyte Interactions and Phagocytosis in Marine Bivalves. 2002. Microscopy Research And Technique 57:469–476
  • Specific Objectives • Identify genes expressed in oyster hemocytes • Characterize response of select molecules following Vibrio challenge
  • Outline • cDNA Library Description • Select Functional Categories • Proteases and Protease Inhibitors • Receptor and Associated Proteins • Transcription/Translation/Cell Cycle
  • Plated hemocyte cDNA library Incubate 24 hours Pacific Oyster Wash in sterile seawater C. gigas Extract with Tri-Reagent + sterile saltwater
  • Plated hemocyte cDNA library Prior to washing After washing
  • Extract from tissue ESTs Make cDNA Gene - DNA (Complementary DNA = Copy) Clone into a “Vector” Transcript - mRNA = cDNA Library Randomly sequence clones = “Expressed Sequence Tags” (ESTs)
  • C. gigas plated hemocytes Roberts et al 2009
  • C. gigas plated hemocytes modified from Roberts et al 2009
  • Markers & more
  • Markers & more
  • C. gigas Vibrio challenge Roberts et al 2009
  • Proteases and Antiproteases Gene Function
  • Proteases and Antiproteases Gene Function
  • Proteases and Antiproteases Gene Function Inhibitors
  • Proteases and Antiproteases Gene Function Cathepsins
  • Proteases and Antiproteases Gene Function Cathepsins
  • Proteases and Antiproteases Gene Function C. virginica Homologs
  • Proteases and Antiproteases Gene C. virginica Homologs
  • Receptors and other proteins Gene Function Prostaglandin E2 EP4 subtype
  • Prostaglandin other ESTs present lipoxygenases production of leukotrienes, lipoxins, hydroperoxy fatty acids 15-hydroxyprostaglandin dehydrogenase PGE2 breakdown
  • Prostaglandin Mackenzie Gavery - Poster
  • Receptors and other proteins Gene Function Ectodysplasin A2 Receptor
  • Transcription / Translation / Cell Cycle Gene Function Erythroid differentiation- related factor
  • Transcription / Translation / Cell Cycle Gene Function High-mobility group proteins
  • Transcription / Translation / Cell Cycle High-mobility group proteins
  • Transcription / Translation / Cell Cycle High-mobility group proteins
  • Conclusions • Several novel genes were identified from C. gigas hemocytes • Hemocyte signaling plays important role in immune response • Stress response is diverse and complex •
  • Conclusions • Several novel genes were identified from C. gigas hemocytes • Hemocyte signaling plays important role in immune response • Stress response is diverse and complex • h/t