NSA08 Roberts Scallop

Bay scallop genetic diversity and population structure in Massachusetts Steven Roberts School of Aquatic and Fishery Sciences University of Washington

Outline •Beginning • Marker Development • Populations / Enhancement • Conclusions

NCBI Present Status Library Characteristics • ESTs = 7057 Individual clones sequenced D-hinge 671 • CoreNucleotides = 85 Met 61 Set 932 Adductor 367 Gonad 58 8% sequences <200 bp • Contigs 845 Average length - 719 bp 970 singletons 217 contigs • Singletons 2746 • Unique Seqs 3591

Enhancement

Population Differences Self-assignment Only Adults Random = 11% Chatham = 60%

Assessing Enhancement private alleles showing up post enhancement

Summary • Identify markers that effectively identify all potential parent populations • functional markers (SSR-ESTs, SNPs) • Isolation coupled with life history can dramatically affect genetic diversity • Temporal (yearly) variation is interesting and critical for understanding diversity

NSA08 Roberts Scallop

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