Bay scallop genetic diversity
and population structure in
Massachusetts
Steven Roberts
School of Aquatic and Fishery Sciences
University of Washington

Outline
•Beginning
• Marker Development
• Populations / Enhancement
• Conclusions

NCBI Present Status
Library Characteristics
• ESTs = 7057
Individual clones sequenced
D-hinge 671
• CoreNucleotides = 85
Met 61
Set 932
Adductor 367
Gonad 58
8% sequences <200 bp
• Contigs 845
Average length - 719 bp
970 singletons
217 contigs
• Singletons 2746
• Unique Seqs 3591

Enhancement

Population Differences
Self-assignment
Only Adults
Random = 11%
Chatham = 60%

Assessing Enhancement
private alleles
showing up post
enhancement

Summary
• Identify markers that effectively identify
all potential parent populations
• functional markers (SSR-ESTs, SNPs)
• Isolation coupled with life history can
dramatically affect genetic diversity
• Temporal (yearly) variation is interesting
and critical for understanding diversity