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Elene Dorfmeier's presentation: How will a changing climate impact Vibrio tubiashii growth and pathogenicity to Pacific oyster larvae?

Elene Dorfmeier's presentation: How will a changing climate impact Vibrio tubiashii growth and pathogenicity to Pacific oyster larvae?

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Elene Dorfmeier pcsga11 Presentation Transcript

  • 1. How will a changing climate impactVibrio tubiashii growth and pathogenicityto Pacific oyster larvae?Elene Dorfmeier, Steven Roberts, Carolyn FriedmanUniversity of Washington | S A F SPCSGA – NSA Joint Meeting 9-21-11
  • 2. Aquaculture and Bivalve Larvae Regional Problems Ü  Limited natural recruitment of bivalve species Ü Ocean chemistry changes Ü Re-emergent disease Photo: Norbert Dankers
  • 3. Acidification of Northwest Waters SUMMER 2009: Dabob Bay Totten InletMap: R. Jacobsen Graphs: Simone Alin et al. NOAA
  • 4. EnvironmentImpact of Acidification on C. gigas Ü Effects of OA on calcifying marine organisms: •  Growth and development •  Energy allocation •  Metabolic depression Ü Pathogen – Host interaction? Ü Disease susceptibility?
  • 5. PathogenVibrio tubiashiiÜ  Gram-negative, facultative anaerobeÜ  Pathogenic to a variety of marine invertebrate larvae, including Pacific oysterÜ  Re-emergent vibriosis in the Northwest Photo: Phetsouvanh et al. 2008
  • 6. Research GoalsÜ  Investigate how environmental stressors will influence Vibrio tubiashii growthÜ  Determine how elevated pCO2 impacts Pacific oyster larval survival and disease susceptibility Photo: Norbert Dankers
  • 7. Research Goalsü  Investigate how environmental stressors will influence Vibrio tubiashii growthÜ  Determine how elevated pCO2 impacts Pacific oyster larval survival and disease susceptibility Photo: Norbert Dankers
  • 8. Vt growth experimental design 25°C 25°C~380 750ppm ppm 12°C 12°C Vt strain RE22 Inoculation dose: 103 CFU/ml 3 days of growth
  • 9. Results: 25°C Vt Growth Error Bars = ± 1 SE
  • 10. Results: Vt Growth at elevated pCO2 Ü  At 25°C, no detectable difference in Vt growth at 750 ppm pCO2 Ü  All stages of growth at 12°C not captured within 72 hr experimentGrowth curves fit to Gompertz growth model: y = Ae(-b2*b3)^x
  • 11. Research GoalsÜ  Investigate how environmental stressors will influence Vibrio tubiashii growthü  Determine how elevated pCO2 impacts Pacific oyster larval survival and disease susceptibility Photo: Norbert Dankers
  • 12. Larval Disease Challenge Temperature: 16°C pCO2: ~380 ppm = 8.0 pH Disease: 750 ppm = 7.8 pH V. tubiashii 2000 ppm = 7.4 pH Ü  Five Vt doses (102 – 106 CFU/ml), plus sterile control Ü  40 larvae per well; 6 replicates per dose of Vt Ü  LD50 determination at 24, 48, and 72 hrs Photo: Ghent University
  • 13. Overview: Vt Disease Challenge Early stage Late stage D-veliger veliger 3 days old 10 days oldPhoto: FAO
  • 14. Results: Larval Disease Challenges Larval Survival on Day 3 Early stage Late Stage Error Bars: ± 95% CI p-values > 0.05
  • 15. LD50 ResultsLD50 reported in CFU/ml of V. tubiashii
  • 16. LD50 ResultsLD50 reported in CFU/ml of V. tubiashii
  • 17. LD50 ResultsLD50 reported in CFU/ml of V. tubiashii
  • 18. Larval Disease Challenge: SummaryNo detectable difference in Pacific oystersusceptibility to vibriosis at elevated pCO2.Considerations: Ø  Larval population variation Ø  Length of exposure to low pH conditions Ø  Carbonate ion availability and saturation states during spawning (Gazeau et al. 2011) Ø  Vt culture conditions
  • 19. Conclusions Ü  Vt growth at 25°C was not significantly different at elevated pCO2 Ü  12°C Vt growth to be performed again to capture full growth curve at 750 ppm Ü  No significant differences in susceptibility to vibriosis were detected at either larval age or pCO2 level in Pacific oysters Ø  Important to consider factors that may affect host susceptibility that are difficult to account for
  • 20. Next Step: Vt Gene Expression VtpR (Hasegawa & Hase 2009) • Metalloprotease Toxins (vtpA) • Hemolysin •  Flagellar Motility expression Photo: NOAA NWFSC
  • 21. THANK YOU!Saltonstall-Kennedy Program (NOAA)UW School of Aquatic & Fishery SciencesEd and Vicky Jones - Taylor Shellfish HatcheryJoth Davis - Taylor ResourcesSam WhiteRichard Wilson – Bay Center MaricultureRussell Rogers – WDFWGenerous student support provided by:Chelsea Farms LLC, Little Skookum ShellfishGrowers, Rock Point Oyster Co., SeattleShellfish, and Taylor Shellfish Co.