How do you find genes
• Entrez (qualifers)
• Sequence similarity (BLAST)
• Large scale BLAST on ESTs
• Degenerative PCR
• SSH, DEG
• Sequencing (can include SSH etc)
Top down approaches to Gene Discovery
Differential Display and Suppression Subtraction
Useful for characterizing transcriptome differences in
different treatments, tissues, stages of development
when there is limited genomic information or a desire to
use a non-hypothesis gene target model.
As opposed to…..
Gene discovery using a degenerative PCR Approach.