Restriction enzymes

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Restriction enzymes

  1. 1. Restriction Enzymes Kasia Fisher Block 3http://www.google.com/imgres?num=10&um=1&hl=en&biw=1015&bih=738&tbm=isch&tbnid=rM0HwLq8CPQYRM:&imgrefurl=http://ww Mrs. Scherman http://www.google.com/imgres?start=34&num=10&u m=1&hl=en&biw=1015&bih=738&tbm=isch&tbnid =13XaF82AY5mieM:&imgrefurl=http://mol-w.simzymes.com/index.php%3Fmain_page%3 biol4masters.masters.grkraj.org/html/Genetic_EngineDindex%26cPath%3D11000_11500&docid=us- ering1B-Molecular_Tools-welDov7Xk6M&imgurl=http://www.simzymes. Restriction_Enzymes.htm&docid=1w2C5_dAjGkvwcom/images//categories/restrictionEnzymes.gif M&imgurl=http://mol-&w=320&h=240&ei=0pO9T9PhGc_16gHh2Ih biol4masters.masters.grkraj.org/html/Genetic_EngineZ&zoom=1&iact=rc&dur=309&sig=103128700 ering1B-Molecular_Tools-879282556508&sqi=2&page=1&tbnh=120&tbn Restriction_Enzymes_files/image001.gif&w=346&hw=160&start=0&ndsp=15&ved=1t:429,r:11,s:0, =257&ei=upS9T53_JqaO6gGXpp1S&zoom=1&iacti:169&tx=110&ty=62 =rc&dur=334&sig=103128700879282556508&page =3&tbnh=158&tbnw=234&ndsp=17&ved=1t:429,r:3 ,s:34,i:54&tx=85&ty=121
  2. 2. What Are Restriction Enzymes?• Enzymes that are produced by different kinds of bacteria that can cleave or cut DNA strands at a specific sequence of nucleotides http://www.google.com/imgres?nu m=10&um=1&hl=en&biw=1015&bih =738&tbm=isch&tbnid=_oThF_Vt0n 85gM:&imgrefurl=http://www.web- books.com/MoBio/Free/Ch9A3.htm &docid=grGZk3ryZHYEpM&imgurl=h ttp://www.web- books.com/MoBio/Free/images/Ch9 At.gif&w=571&h=434&ei=0pO9T9Ph Gc_16gHh2IhZ&zoom=1&iact=hc&v px=90&vpy=357&dur=1241&hovh=1 96&hovw=258&tx=179&ty=109&sig =103128700879282556508&sqi=2& page=1&tbnh=120&tbnw=158&start =0&ndsp=15&ved=1t:429,r:10,s:0,i: 167
  3. 3. Types of Restriction Enzymes• Type I: Random cuts occur at locations that could be thousands of nucleotides apart• Type III: Cuts are as long as around 25 nucleotides• Type II: This type is most used in Biology – cuts occur about every 4-6 nucleotides
  4. 4. Types of Cleaving• Blunt End: Enzyme • Sticky End: Enzyme cuts straight through cuts closer to each side of the recognition sequence. http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.html
  5. 5. Discovery• Werner Arber & Stuart Linn discovered Restriction Enzymes• 1st Restriction Enzyme that was discovered was EcoB – This came from a strain of E. coli – Soon after this discovery, EcoK was discovered from E. coli as well
  6. 6. Recombinant DNA Process• In this process, bacterial DNA is joined with a gene of interest• This process is performed in order to study the DNA more thoroughly or for useful products like vaccines
  7. 7. Recombinant DNA Process Steps1. DNA from the organism of interest is prepared to be able to be cloned2. A virus is digested with and enzyme and compatible ends (with the DNA) are made3. DNA in a plasmid form is cut4. This DNA is joined with the virus’ compatible ends5. The DNA strands are joined with DNA ligase
  8. 8. Recombinant DNA Process http://www.google.com/i mgres?num=10&um=1&h l=en&biw=1015&bih=73 8&tbm=isch&tbnid=ht1Y nB2I- Plasmid is cut here 9Q5HM:&imgrefurl=http: //en.wikipedia.org/wiki/Re combinant_DNA&docid= KUT- 5jVGGyn4zM&imgurl=ht tp://upload.wikimedia.org/ wikipedia/en/thumb/0/01/ Recombinant_formation_ of_plasmids.svg/280px- Recombinant_formation_ of_plasmids.svg.png&w= 280&h=227&ei=xJe9T6D oELON6QHcvZhe&zoom =1&iact=hc&vpx=127&v py=201&dur=204&hovh= 181&hovw=224&tx=114 &ty=110&sig=103128700 879282556508&sqi=2&p age=1&tbnh=153&tbnw= 189&start=0&ndsp=15&v ed=1t:429,r:0,s:0,i:100 Here, the newCompatible DNA is attachedvirus ends are with DNA ligasejoined
  9. 9. Still Confused?• The beginning of this video shows how Restriction Enzymes work to create Recombinant DNA:http://www.youtube.com/watch?v=YdjvUv-1vCI
  10. 10. Sources• http://users.rcn.com/jkimball.ma.ultranet/Bio logyPages/R/RestrictionEnzymes.html• http://biotech.about.com/od/proteinengineer ing/a/restrictenz.htm• http://www.bookrags.com/research/restrictio n-enzymes-gen-04/

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