Restriction enzymes
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Restriction enzymes

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    Restriction enzymes Restriction enzymes Presentation Transcript

    • Restriction Enzymes Kasia Fisher Block 3http://www.google.com/imgres?num=10&um=1&hl=en&biw=1015&bih=738&tbm=isch&tbnid=rM0HwLq8CPQYRM:&imgrefurl=http://ww Mrs. Scherman http://www.google.com/imgres?start=34&num=10&u m=1&hl=en&biw=1015&bih=738&tbm=isch&tbnid =13XaF82AY5mieM:&imgrefurl=http://mol-w.simzymes.com/index.php%3Fmain_page%3 biol4masters.masters.grkraj.org/html/Genetic_EngineDindex%26cPath%3D11000_11500&docid=us- ering1B-Molecular_Tools-welDov7Xk6M&imgurl=http://www.simzymes. Restriction_Enzymes.htm&docid=1w2C5_dAjGkvwcom/images//categories/restrictionEnzymes.gif M&imgurl=http://mol-&w=320&h=240&ei=0pO9T9PhGc_16gHh2Ih biol4masters.masters.grkraj.org/html/Genetic_EngineZ&zoom=1&iact=rc&dur=309&sig=103128700 ering1B-Molecular_Tools-879282556508&sqi=2&page=1&tbnh=120&tbn Restriction_Enzymes_files/image001.gif&w=346&hw=160&start=0&ndsp=15&ved=1t:429,r:11,s:0, =257&ei=upS9T53_JqaO6gGXpp1S&zoom=1&iacti:169&tx=110&ty=62 =rc&dur=334&sig=103128700879282556508&page =3&tbnh=158&tbnw=234&ndsp=17&ved=1t:429,r:3 ,s:34,i:54&tx=85&ty=121
    • What Are Restriction Enzymes?• Enzymes that are produced by different kinds of bacteria that can cleave or cut DNA strands at a specific sequence of nucleotides http://www.google.com/imgres?nu m=10&um=1&hl=en&biw=1015&bih =738&tbm=isch&tbnid=_oThF_Vt0n 85gM:&imgrefurl=http://www.web- books.com/MoBio/Free/Ch9A3.htm &docid=grGZk3ryZHYEpM&imgurl=h ttp://www.web- books.com/MoBio/Free/images/Ch9 At.gif&w=571&h=434&ei=0pO9T9Ph Gc_16gHh2IhZ&zoom=1&iact=hc&v px=90&vpy=357&dur=1241&hovh=1 96&hovw=258&tx=179&ty=109&sig =103128700879282556508&sqi=2& page=1&tbnh=120&tbnw=158&start =0&ndsp=15&ved=1t:429,r:10,s:0,i: 167
    • Types of Restriction Enzymes• Type I: Random cuts occur at locations that could be thousands of nucleotides apart• Type III: Cuts are as long as around 25 nucleotides• Type II: This type is most used in Biology – cuts occur about every 4-6 nucleotides
    • Types of Cleaving• Blunt End: Enzyme • Sticky End: Enzyme cuts straight through cuts closer to each side of the recognition sequence. http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.html
    • Discovery• Werner Arber & Stuart Linn discovered Restriction Enzymes• 1st Restriction Enzyme that was discovered was EcoB – This came from a strain of E. coli – Soon after this discovery, EcoK was discovered from E. coli as well
    • Recombinant DNA Process• In this process, bacterial DNA is joined with a gene of interest• This process is performed in order to study the DNA more thoroughly or for useful products like vaccines
    • Recombinant DNA Process Steps1. DNA from the organism of interest is prepared to be able to be cloned2. A virus is digested with and enzyme and compatible ends (with the DNA) are made3. DNA in a plasmid form is cut4. This DNA is joined with the virus’ compatible ends5. The DNA strands are joined with DNA ligase
    • Recombinant DNA Process http://www.google.com/i mgres?num=10&um=1&h l=en&biw=1015&bih=73 8&tbm=isch&tbnid=ht1Y nB2I- Plasmid is cut here 9Q5HM:&imgrefurl=http: //en.wikipedia.org/wiki/Re combinant_DNA&docid= KUT- 5jVGGyn4zM&imgurl=ht tp://upload.wikimedia.org/ wikipedia/en/thumb/0/01/ Recombinant_formation_ of_plasmids.svg/280px- Recombinant_formation_ of_plasmids.svg.png&w= 280&h=227&ei=xJe9T6D oELON6QHcvZhe&zoom =1&iact=hc&vpx=127&v py=201&dur=204&hovh= 181&hovw=224&tx=114 &ty=110&sig=103128700 879282556508&sqi=2&p age=1&tbnh=153&tbnw= 189&start=0&ndsp=15&v ed=1t:429,r:0,s:0,i:100 Here, the newCompatible DNA is attachedvirus ends are with DNA ligasejoined
    • Still Confused?• The beginning of this video shows how Restriction Enzymes work to create Recombinant DNA:http://www.youtube.com/watch?v=YdjvUv-1vCI
    • Sources• http://users.rcn.com/jkimball.ma.ultranet/Bio logyPages/R/RestrictionEnzymes.html• http://biotech.about.com/od/proteinengineer ing/a/restrictenz.htm• http://www.bookrags.com/research/restrictio n-enzymes-gen-04/