Fungi Experiment
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Fungi Experiment

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Fungi Experiment Fungi Experiment Presentation Transcript

  • Review of slides in mycology Pictures c/0 Karentan.Content c/o chumtemp. OLFU Med II 2008
  • Dermatophytosis/ cutaneous mycoses “ Tineas”
  • FUNGIFACTS: DERMATOPHYTES Genus Species Macroconidia Microconidia Disease(s) Produced Epidermophyton floccosum Numerous Smooth- walled Absent Tinea corporis, pedis, ungium, cruris Microsporum canis Numerous thick walled Rough POINTED ENDS (+)Spiny tips Rare Tinea capitis Tinea corporis Microsporum gypseum Numerous thiN walled Rough BLUNT ENDS Rare Tinea capitis Tinea corporis Trichophyton mentagrophytes Rare, thin walled, smooth *Hypha: COILED Grape-like Tinea pedis, ungium, cruris, barbae Trichophyton Rubrum Absent Teardrop Tinea corporis, pedis, ungium, cruris
  •  
  • Epidermophyton floccosum Numerous, smooth walled MACROconidia
    • Thick-walled, pointed end MACROconidia
    Microsporum canis With spiny tips
  • Microsporum gypseum
    • Blunt end MACROconidia
  • Trichophyton mentagrophytes Grape-like clusters (“en grappe”) microconidia macroconidia *Coiled hyphae
  • Trichophyton rubrum
    • Tear drop (enthyrse) microcondia
  • systemic mycosis Histoplasmosis
  • Histoplasma capsulatum tuberculate MACROconidia
    • FUNGIFACTS
    • Histoplasmosis
    • Laboratory Diagnosis:
    • KOH: organisms found inside histiocytes
    • Wright/Giemsa
    • Culture: SDA, BAP (Smith & Goodman)
    • Serology
      • Skin test: Histoplasmin Ag
      • CF
  • opportunistic mycoses
    • Aspergillosis
    • Candidiasis
    • Cryptpococcosis
    • Mucormycosis
    • Penicilliosis
  • Aspergillus fumigatus conidiophore conidia vesicle Biseriate phialide - phialide - metula
    • FUNGIFACTS
    • Aspergillosis
    • (+) Fungus ball - spherical mass of intertwined septate branching hyphal elements
    • Laboratory Diagnosis:
    • septate hyphae that branch at regular intervals seen in clinical specimens
  • Candida albicans
    • FUNGIFACTS
    • *Chronic Mucocutaneous Candidiadis
    • Laboratory Diagnosis:
      • (+) germ tube test (rapid diagnosis)
      • (+)Budding yeast cells
      • (+)pseudohyphae
      • (+)septate hyphae
    Budding yeasts Germ tubes
  • Cryptococcus neoformans Encapsulated yeast
    • FUNGIFACTS
    • Cryptococcosis
    • Laboratory Diagnosis:
    • India ink: encapsulated yeast
  • Mucor sp.
    • Sporangium
    • *sporangiospores inside
    • FUNGIFACTS
    • Mucormycosis
    • Laboratory Diagnosis:
    • (+)coenocytic hyphae
    • (+) sporangium containing sporangiospores
    • * Ubiquitous in the environment and encountered as contaminants
    sporangiospores Coenocytic (aseptate) hyphae
  • Rhizopus sp. sporangiospores sporangium rhizoids
    • FUNGIFACTS
    • Mucormycosis
    • Laboratory Diagnosis:
    • (+) RHIZOIDS
    • (+) sporangium containing sporangiospores
  • Penicillium sp. Chains of conidia Branching conidiophore Phialides
  • Subcutaneous mycoses Sporotrichosis Chromomycosis
  • Sporothrix schenckii Daisy-like microconidia
    • FUNGIFACTS
    • Sporotrichosis (Lymphocutaneous)
    • Dimorphic
      • Yeast: cigar body (budding yeast cells)
      • Mold: numerous micro conidia in a rosette/daisy pattern
  • Cladosporium carrionii Long chain of conidia
    • FUNGIFACTS
    • Chromomycosis
    • Dematiaceous
      • Mold: branching conidiophore
      • Chains of conidia
      • Septate hypha
  • Phialophora verrucosa Flask-shaped conidipohore
    • FUNGIFACTS
    • Chromomycosis
    • Verrucous dermatitis
    • Dematiaceous
      • Septate hypha w/ short conidiophore & collarate mass of conidia
  • Fonseca pedrosoi Club-shaped conidiophore Pattern of sporulation: acrotheca Conidia @ lateral sides