Afb microscopy and quality assurance copyPresentation Transcript
BRONCHIAL WASH CENTRIFUGE
BRONCHOALVEOLAR LAVAGE 3000 RPM × 15MINS
GASTRIC L AVAGE NEUTRALISATION
higher concentration fuchsin
longer staining time errors !!
NOT RECOMMENDED for low-income countries
With applicator stick from the thick/yellowish part make 15 mm by 20 mm oval smear
Once dried in air pass the slides 2-3 times over flame or by sprit for 3 mins
Cover the slide with Carbol fuschin 0.3% after filtering through Whatman filter paper no.1
Heat the slide until it steams and continue it for 5 minutes (don’t allow the stain to dry)
Wash it under tap water
Cover the slide with 3% acid alcohol (HCl and 95% alcohol or 20% Sulphuric acid (1 minute)
Counterstain with malachite green for 1 minute
Each lab staff must know that the reagents for staining are highly corrosive substances and must ensure personal protection
Efforts must me made to minimize and control lab operations that induce potentially infectious aerosols
Opening of container/preparing smears on slides/flaming
Use safety cabinet always!!
Sterilize the loop after smear
Note: Color of AFB may vary with filter system on microscope
Counter Stain Background
Accuracy : The closeness of measurements to the true value. Precision : The amount of variation in the measurements. Bias: The difference between the expectation of a test result and an accepted reference value.