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Napoleon Personal Profile Aug 2009 Final Version
 

Napoleon Personal Profile Aug 2009 Final Version

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A short presentation highlighting my Graduate Experience (Research, Teaching & Coursework) with a brief Overview of my Undergraduate degree.

A short presentation highlighting my Graduate Experience (Research, Teaching & Coursework) with a brief Overview of my Undergraduate degree.

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    Napoleon Personal Profile Aug 2009 Final Version Napoleon Personal Profile Aug 2009 Final Version Presentation Transcript

    • ECC International RESEARCH EXPERIENCE :: GRADUATE COURSEWORK :: TEACHING EXPERIENCE Mudiarasan Napoleon : : Personal Profile
    • Agenda
      • Research Experience (M.S Degree)
        • Introduction & Background
        • Research Projects - An Overview
        • Core Competencies
      • Graduate Coursework
      • Teaching Experience
      • B.Tech [IBT] :: An Overview
    • Research Experience – M.S Degree
      • Model Organism : Flavobacterium johnsoniae
      • Characteristics: Gram Negative, rod-shaped gliding bacteria. Occurs widely in soil and water.
      • Research Focus: Identification of components of the gliding machinery of F. johnsoniae
      • Research Methodology: A genetic approach to characterize all the genes and proteins involved.
      • Techniques Used: Genetics, Molecular Biology, Biochemistry, Microscopy and Bioinformatics
      • Major Professor: Dr Mark J McBride, University of Wisconsin-Milwaukee, USA
      3
    • Research Experience – M.S Degree Research Publications: 2 Manuscripts for the Papers on characterization of spr E and gldN, gldO genes are currently under preparation in Wisconsin, USA. 4 Title Research Projects – An Overview
      • Dates (from – to)
      • Project Name
      • Project Details
      December 2006 – August 2008 Characterization of the gldN and gldO genes in gliding motility of Flavobacterium johnsoniae. Created the first double knockout mutant in the gld genes, which play a vital role in gliding motility. The gldNO knockout mutant was generated using a novel phage selection method. Complementation and phenotypic analysis confirmed their involvement indicating that GldN and GldO are partially redundant components of the motility machinery.
      • Dates (from – to)
      • Project Name
      • Project Details
      May 2005 - December 2006. Characterization of spr E gene of Flavobacterium johnsoniae in gliding motility & chitin utilization. Transposon mutagenesis was used to isolate the sprE mutants. The sprE and the downstream fjoh_1052 genes were successfully cloned into DNA constructs . Complementation studies demonstrated the involvement of sprE in gliding motility. Bioinformatic analysis predicted SprE to be a lipoprotein with six TPR domains, which are likely to be involved in protein-protein interactions.
      • Dates (from – to)
      • Project Name
      • Project Details
      January 2005 - May 2005. Complementation analysis of spontaneous point mutants of Flavobacterium johnsoniae using gld A gene. A bank of 37 different point mutants was screened for mutation in gld A gene and checked for restoration of the wild type phenotype. One of them was successfully complemented indicating the presence of a gld A point mutation.
    • OM CM F F A A G G J B F. johnsoniae Motility Proteins K N M O L SprB SprA SprD SprC SprE
      • Boxed Proteins were Characterized in my research.
      5 ATP ADP
    • Research – Core Competencies 6
      • Molecular Cloning
      • Mutagenesis
      • Gene Transfer Methods
      • Construction of DNA libraries
      • Isolation of DNA
      • Microscopy
      • Polymerase Chain Reaction
      • Aseptic Microbial Techniques
      • Gel Electrophoresis
      • Characterization of Microbes
      • Protein Expression
      • Animal Dissection
      • Western Blot Analysis
      • Sequence Analysis using Bioinformatics tools
    • Graduate Coursework – M.S Degree
      • Coursework List: *
      • Introduction to Biochemistry
      • Protein Structure and Function
      • Biochemistry: Cellular Processes
      • Biological Electron Microscopy
      • Frontiers in Biotechnology
      • Microbial Transport & Gene Regulation
      • Biochemistry: Metabolism
      • Analysis of Genes & Genomes [Bioinformatics]
      • Molecular Biology
      • Microbial Diversity and Physiology
      • Highlights of Coursework:
      • Completed 55 Credits [37-Coursework; 18-Research]
      • Diverse coursework
      • Topped the university in the courses highlighted
      • Delivered in-class presentations
      • Presented papers in various seminars
      • Engaged in Initiatives to enhance learning
      * Listed Coursework is not exhaustive 7
    • Teaching Experience
      • Laboratory & Discussion Courses Taught:
      • 101 General Survey of Microbiology *
      • 383 General Microbiology *
        • Aseptic Microbial Techniques
        • Isolation & Characterization of Microbes
        • Microscopy & Staining Procedures
      • 202 Anatomy & Physiology Part – I *
      • 203 Anatomy & Physiology Part – II *
        • Cell & Tissue Biology
        • Organs & Organ Systems
        • Fetal Pig Dissection
      • Duties & Responsibilities:
      • Lectured on Theoretical Concepts
      • Demonstrated Experiments
      • Trained students in various Techniques
      • Designed Exams & Quizzes
      • Proctored and Graded Exams, Lab Reports
      • Moderated In-Class Projects
      • Observed Office Hours to Resolve Queries
      • Discussion Sessions to clarify Doubts
      • Facilitated Special Accommodations for Disabled Students
      * Only Selected Topics are Listed 8
    • B.Tech [Industrial Biotechnology] : : An Overview
      • B.Tech [Industrial Biotechnology] from A.C.Tech, Anna University, Chennai, India.
      • An Unique Interdisciplinary program with equal focus on the Biological, Physical and Chemical Sciences. First of its’ kind in the nation.
      • An integrated focus of the basic Sciences and Engineering processes involved in the development of Biotechnology based products.
      • 197 Credits of undergraduate coursework with a breadth of electives.
      • Ranked 15 th in the All India Entrance Exam conducted by AU-JHU, for admission in to the B.Tech [Industrial Biotechnology] program.
      • Among the top 1% of candidates selected by the Tamil Nadu Professional Course Entrance Exam [TNPCEE].
      Thank you !!! 9