Biotecnología aplicada a cultivos de microalgas

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En el marco de la jornada Microalgas, ¿una fuente de petróleo verde?, organizada con IMDEA y celebrada el 8 de abril en EOI, Escuela de Organización Industrial, Antonio Fernández del Instituto Biomar, como se usa la biotecnología a cultivos de microalgas.
8_04_2010

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Biotecnología aplicada a cultivos de microalgas

  1. 1. BIOTECNOLOGIA APLICADA AL CULTIVO DE MICROALGAS: BUSQUEDA, SELECCIÓN Y OPTIMIZACION DE MICROALGAS PARA LA PRODUCCION DE ACEITE COMO BIOCOMBUSTIBLE. BIOTECHNOLOGY APPLIED TO MICROALGAE CULTURES: SELECTION AND OPTIMIZATION OF MICROALGAE CULTURES FOR BIODIESEL PRODUCTION.
  2. 2. Instituto Biomar is a 14 year old biotechnological company located in Parque Tecnológico de León The company
  3. 3. 1996: Spin off from PharmaMar 1997: Move to facilities in biotech business incubator 2000: Start of work in Industrial Applications: Biospesticides 2001: Start of the Fermentation plant 2005: Start of the Microalgae project 2007: Start of the “Microalgae for biodiesel” project. 2009: Opening of new facilties in Leon Technological Park The company
  4. 4. TECHNOLOGIES APPLICATIONS The company Instituto Biomar: fields of interest MICROBIOLOGY CHEMISTRY FERMENTATION HUMAN HEALTH FOOD BIOPESTICIDES AQUICULTURE BIODIESEL
  5. 5. The company
  6. 6. The company
  7. 7. The company
  8. 8. BIODIESEL Metanol + KOH Trans- esterification Crude biodiesel Glicerol Metanol <ul><li>Proteins </li></ul><ul><li>Sugars </li></ul><ul><li>High value compounds </li></ul>Photosynthetic microorganisms (Biomass) Acylglicerides Microorganisms Actinobacteria Fungi Biodiesel Project
  9. 9. Biodiesel Project STRAIN SELECTION GROWTH RATE OIL CONTENT OPTIMIZATING PRODUCTION CLASSIC METHODS GENETIC MANIPULATION INDUSTRIAL SCALE OPEN SYSTEMS (RACEWAYS) CLOSED- SYSTEMS (PHOTO-REACTORS)
  10. 10. CO 2 Lipids Chemical transformation Water Salts MICROALGAE BIOMASS BIODIESEL EXTRACTION Sugars, proteins High value compounds Biodiesel Project
  11. 11. Isolation from marine invertebrate organisms, water, sediments or soils and plant samples of different sites of the world (Europe, America, Africa…) Biodiesel Project Australia Mediterranean Sea Japan Ecuatorial Guinea Canarias Escocia Hawaii Red Sea Spain Senegal Argentina Puerto Rico Panamá Guatemala Martinica México Alaska New Zeland Norway Island Chile Perú Groenland
  12. 12. Laboratory of Microalgae ISOLATION Marine macroorganisms, sediments, rocks, soils…are crushed and cultured on different media (BG-11, ASNIII,…). Isolation process : Picking colonies by hand under stereomicroscopes. ( 3000 isolated monocultures per year )
  13. 13. STRAIN SELECTION Dereplication process by sample and sampling point, using stereomicroscope and microscope. This process leads to different strains (aproximately 1200-1400 different monocultures per year). Laboratory of Microalgae Current collection of microalgae and cyanobacteria is approximately 2.500 monocultures
  14. 14. STRAIN CULTURE PRIMARY CULTURES on 50 ml flasks are cultured with controlled light and temperature (1200-1400 monocultures per year). Laboratory of Microalgae
  15. 15. STRAIN CULTURE (2) SECONDARY CULTURES on 200 mL tubes, aireated and with temperature and light controlled (1200-1400 monocultures per year). Laboratory of Microalgae
  16. 16. STRAIN CONTROL All strains are photographed, growth is measured, dry weight is estimated, HPLC (UV-vis) profile is determined and DNA is obtained. With these secondary cultures we obtain biomass for our screening programs: - Drug discovery screening program. - Biodiesel screening program. Laboratory of Microalgae
  17. 17. SCREENING Acylglicerides measurements Samples are extracted with hexane and measured for acylgliceride content (not lipid content) by colorimetry (595 nm) on 96 multiwell plates. Laboratory of Microalgae
  18. 18. SCREENING Positive samples are re-tested using TLC in order to diferentially detect acylglicerides (tri-, di-, monoacylglicerides). TAG DAG MAG Laboratory of Microalgae
  19. 19. We select 10-20 strains per year to initiate studies in order to improve growth and/or productivity. SELECTING STRAINS Laboratory of Microalgae mg DW · L -1 /100
  20. 20. <ul><li>IMPROVEMENT </li></ul><ul><li>Growth: Testing different conditions on 24 well plates. </li></ul><ul><li>Acylglicerides content: searching for conditions leading to accumulation but with no effects on growth. </li></ul>Laboratory of Microalgae
  21. 21. SCALE-UP From these studies we aim to select 1-2 strains with appropiate characteristics to initiate open air cultures (200-400 L) during 2010, as a step previous to industrial scale up. Laboratory of Microalgae
  22. 22. 1 2 Isolating 3 4 Culturing 5 Caracterizing 8 7 6 Selecting Improving Producing strain DYNAMIC PROCESS Bioprospecting Profiling Biodiesel project
  23. 23. Biodiesel project STRAIN SELECTION GROWTH RATE OIL CONTENT OPTIMIZATING PRODUCTION CLASSIC METHODS GENETIC MANIPULATION INDUSTRIAL SCALE OPEN SYSTEMS (RACEWAYS) CLOSED- SYSTEMS (PHOTO-REACTORS)

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