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Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization
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Dynamic Thermoelectric Glucose sensing with Layer-by-Layer Glucose Oxidase Immobilization

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  • Detecting the loss or generation of stoichiometrically related chemical species with an appropriate electrode. Stability of sensor depends upon the stability of the immobilized enzyme.
  • Coefficient of determination or squared of correlation coefficient.
  • Transcript

    • 1. Dynamic Thermoelectric Glucose Sensing with Layer-by-Layer Glucose Oxidase Immobilization
      Siva Mahesh Tangutooru1
      V.L. Kopparthy, R. Gumma, G.G. Nestorova, E.J. Guilbeau
      Center for Biomedical Engineering and Rehabilitation Science
      Louisiana Tech University
    • 2. Goal
      To develop a microfluidic glucose calorimeter by immobilizing glucose oxidase using layer-by-layer self-assembly.
    • 3. Presentation Summary
      Principle of thermoelectricity
      Calorimeter design and measurement system
      Layer-by-layer self-assembly procedure
      Results
      Conclusion
      Future work
    • 4. Thermoelectric detection of glucose
      𝐺𝑙𝑢𝑐𝑜𝑠𝑒+𝑂2+𝐻2𝑂𝑔𝑙𝑢𝑐𝑜𝑠𝑒 𝑜𝑥𝑖𝑑𝑎𝑠𝑒𝐺𝑙𝑢𝑐𝑜𝑛𝑖𝑐 𝑎𝑐𝑖𝑑+𝐻2𝑂2+79 𝑘𝐽/𝑚𝑜𝑙
       
    • 5. Outlet
      Inlet1
      Device fabrication
      Inlet2
      Thermopile
      Microfluidic Device
      Microfluidic Calorimeter
      PDMS inlet & outlet connectors
      Microscope glass slide
      Kapton® tape
      Microscope glass coverslip
      (a)
      (a)
      (b)
      (c)
      (d)
    • 6. Experimental measurement system
    • 7. Hydrodynamic focusing
      G. G. Nestorova and E. J. Guilbeau, "Thermoelectric method for sequencing DNA," Lab on a Chip, vol. 11, pp. 1761-1769.
    • 8. PEI
      Layer-by-layer self assembly
      PSS
      Glucose Oxidase
      PEI
      Layer-by-layer electrostatic adsorption mechanism.
      General adsorption procedure of ployelectrolytes on a substrate.
      Layer-by-layer assembly of glucose oxidase on the substrate.
      Structure of polyelectrolytes used for LbL assembly.
      N+
      H2
      SO3- Na+
      Polyelectrolyte
      Glucose
      Oxidase
      Immobilization procedure for LbL assembly.
      K. Ariga, Y. Lvov “Self-Assembly of Functional Protein Multilayers: From Flat Films to Microtemplate Encapsulation”, “Biopolymers at Interfaces” Ed. M. Malmsten, M. Dekker Publ., 2003, NY, p.367-391.
      (a)
      PSS
      PEI
      (c)
      (b)
    • 9. Our novel glucose calorimeter
      Calorimeter
      Self-generating signal
      No external power requirement
      Relatively inexpensive
      Small in size and light weight
      High rejection of common mode thermal signals
      Relatively simple to manufacture
      Rugged and durable
      Layer-by-layer Immobilization
      Multiple layers are physically adsorbed.
      Easy preparation and high bond strength.
    • 10. Sensor response for glucose concentration
      75 mg/dL
      100 mg/dL
      Flow rates: 100 µlmin-1 and 25 µlmin-1
      No. of Immobilized glucose oxidase layers: 2
    • 11. Effect of number of immobilized layers on sensor response
      Flow rates: 100 µlmin-1 and 25 µlmin-1
    • 12. Effect of flow rates on sensor response
      No. of Immobilized glucose oxidase layers: 2
    • 13. Conclusion
      Successfully immobilized multiple layers of glucose oxidase using LbL self-assembly.
      Multiple layers of glucose oxidase had little effect because oxygen limited the reaction.
      Increasing the ratio of inlet flow rates improved calorimeter response.
      Future work
      This thermoelectric method can be employed to detect the enthalpy produced by enzymatic reactions.
      glutamate concentration detection in the flow stream by immobilizing glutamate oxidase using layer-by-layer self-assembly.
    • 14. Thank you
      Questions ???
    • 15. M. J. Muehlbauer, E. J. Guilbeau, and B. C. Towe, "Applications and stability of a thermoelectric enzyme sensor," Sensors & Actuators: B. Chemical, vol. 2, pp. 223-232, 1990.

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