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This Powepoint introduced the applications of BioTechnology & Microbiology in Flotation.

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  1. 1. University of Tehran Collage of Engineering School of Mine By: Sina Ghassa Advisor: Professor Gharabaghi December 2013
  2. 2. 2 Cultivation of bacteria Bacteria Classifications Bio-flotation Fundamentals Bio-flotation Applications
  3. 3. BACTERIA CLASSIFICATION (FEEDING) 3 Autotrophic Bacteria Use atmosphere CO2 as Carbon resource Use ammoniac as Nitrogen resource Heterotopic Bacteria Farina, Glucose, and other nutrient have to added to cultures Bacteria could Classified based on: Feeding Requirements Shape Living Temperature
  4. 4. BACTERIA CLASSIFICATION (SHAPE) 4 Bacillus (A) Spirillum (B) Cocci (C) (B) (C)
  5. 5. BACTERIA CLASSIFICATION (TEMPERATURE ) 5 Mesophil 25-45 C Acidobacillus Ferrooxidans, Acidobacillus thiooxidans, Leptospillirum Ferrooxidans Moderate Thermophile 45-65 C Most of them are heterotrophic bacteria Thermophile 65-85 C Sulfolobus Extremely Thermophile Upper than 85 C
  6. 6. CULTIVATION OF BACTERIA 6  Steps 1. Enrichment Step 2. Separation Step 3. Purification Step  Culture Media 1. Solid culture 2. Liquid
  7. 7. CULTURE MEDIA 7 D1D2LeathenNorrisHP0.9K9K (NH4)SO4 MgSO4. 7H2O K2HPO4 --KCl -----Ca (NO3)2. H2O Elemental Sulfur or Ferric could be added to media as nutrient Culture Media Compositions
  10. 10. SURFACE CHANGES 10 The surface changes of sphalerte particle in contact with bacteria (Ghassa et al. 2014)
  11. 11. BIO-FLOTATION 11 The Bio-Flotation have been used to reduce the using chemical reagents to reduce the environmental impacts and microorganisms selectivity Bacteria could be used as: Flotation depressants Collectors Dispersing agent Flocculate
  12. 12. MECHANISMS 12 There are three different mechanisms by means of which the biomodification can occur: attachment of microbial cells to the solid substrate oxidation reactions adsorption and/or chemical reaction with the metabolite products (EPS).
  13. 13. BACTERIA ADHESION 13 The bacterial adhesion occurs as a net result of attractive and repulsive forces of the cell and mineral surfaces. The interactions that result in such adhesion include electrostatic interactions, acid–base interactions, van der Waals forces and hydrophobic interactions, all of which are determined by the cell-wall and mineral surface properties (Merma et al. 2013)
  14. 14. DEPRESSANTS 14 The selective flotation separation of cinnabar from antimonite has been carried out by Lyalikova & Lyubavina (1986) using A. ferrooxidans. They suggested that antimonite was oxidized by the bacteria, leading to its depression, while cinnabar was not affected. It was found that galena was totally depressed in the pH range of 5-11 after bacterial interaction, while the flotation recovery of sphalerite was not affected. The significant differences in the adsorbabilities of the bacterial cells onto galena and sphalerite coupled with the nature of the interaction products, be it the respective sulfates or hydroxides,
  15. 15. PYRITE DEPRESSANTS 15 Cyanide have been used extensively as Pyrite Depressants in flotation processes In the presence of A.thiobacillus Ferrooxidans, and xanthate as collector, pyrite was depressed(40%),whereas chalcopyrite and other sulfide minerals were unaffected at natural pH (Hosieni et al., 2005) The Pyrite recovery in presence of A.thiobacillus Ferrooxidans was 8% during Galen concentrating (Mehrabani et al., 2011) During Sphalerite concentrating the pyrite recovery is 23.52 %.
  16. 16. COLLECTOR 16  Bacillus subtilis and Mycobacterium phlei function as collector in anionic collector flotation of dolomitic phosphate ores, while Bacillus subtilis functions as the stronger collector, especially for dolomite  The interaction of P. polymyxa with calcite, hematite, corundum, kaolinite and quartz resulted in the quartz and kaolinite surfaces being rendered more hydrophobic
  17. 17. BIO-FLOCCULATION 17  Mycobacterium phlei was able to flocculate phosphate slimes, hematite and coal (Smith et al., 1991)  Produce extracellular polymers and surfactants under certain conditions, which can cause flocculation of the microorganisms themselves or of other solids
  18. 18. REFERENCES 18 Donati, E. R., 2007. Microbial Processing of metal sulfides, Springer Hosseini, T.R., Kolahdoozan, M.,. Tabatabaei, Y.S.M, Oliazadeh, M., Noaparast, M., Eslami, A., Manafi, Z., Alfantazi. A., 2005. Bioflotation of Sarcheshmeh copper ore using Thiobacillus Ferrooxidans bacteria. Minerals Engineering 18, 371–374 Mehrabani, J.V., Mousavi, S.M., Noaparast, M. 2011. Evaluation of the replacement of NaCN with Acidithiobacillus ferrooxidans in the flotation of high-pyrite, low-grade lead–zinc ore. Separation and Purification Technology 80, 202–208 Subramanian, S., Santhiya, D., Natarajan, K.A. 2003. Surface modification studies on sulphide minerals using bioreagents. Int. J. Miner. Process. 72, 175– 188 Farahat, M., Hirajima,t., Sasaki, K., Aiba, y., Doi, K., 2008. Adsorption of SIP E. coli onto quartz and its applications in froth flotation. Minerals Engineering 21, 389–395
  19. 19. 19 SINA GHASSA University of Tehran December 2013