Surface Activated Chemical Ionization – applications to low molecular weight drugs analysis Simone Cristoni
Surface Activated Chemical Ionization
Surface Activated Chemical Ionization Controlled, low potential Metal surface
SACI <ul><li>High sensitivity </li></ul><ul><li>High Versatility (different eluent flow rates, compounds and matrix) ‏ </l...
Analysis of addictive drugs <ul><li>Urine </li></ul><ul><li>Hair </li></ul><ul><li>Saliva </li></ul><ul><li>Sample dilutio...
Analysis of addictive drugs <ul><li>Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisiti...
Analysis of addictive drugs Urine 2 ng/mL APCI SACI
Analysis of addictive drugs Saliva 10 ng/mL of 6-MAM 1 ng/mL of 6-MAM 4 Retention time (min)‏ 14 a) SACI‏ Signal intensity...
Analysis of pesticides <ul><li>Water </li></ul><ul><li>Spinach </li></ul><ul><li>Sample dilution 1:10 </li></ul>Avoiding p...
Analysis of pesticides <ul><li>Mass spectrometer: ESI-TOF (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisition mode: High ma...
Analysis of pesticides water
Analysis of pesticides spinach
Analysis of toxin in food <ul><li>Wine </li></ul><ul><li>Grape </li></ul><ul><li>Sample dilution 1:10 </li></ul>Avoiding p...
Analysis of pesticides <ul><li>Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisition mo...
Analysis of pesticides spinach
Analysis of OTA Wine – 10 ng/mL
Conclusions <ul><li>High sensitivity (LOD, LOQ, linerity ranges) ‏ </li></ul><ul><li>Cost effecting avoiding matrix effect...
Future developments <ul><li>Ion exchange chromatography (low operative voltages employed in SACI makes possible to operate...
SACI demo site Web site: http://www.isbiotechnology.com
acknowledgment <ul><li>Dr.ssa Sara Crotti – ISB Milan </li></ul><ul><li>Dr. Lorenzo Zingaro – ISB Milan </li></ul><ul><li>...
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Saci Short Description And Applications

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This is a short presentation that shows the working mechanism and the applications of the new ionization source, for mass spectrometry, named Surface Activated Chemical Ionization (SACI). More informations can be found at the URL:
http://www.isbiotechnology.com

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  • Title of sales presentation – Remarks: „Bruker Confidential“ needs to be erased from the master slide for external use. Some alternative illustrative pictures are collected on the last slide of this master.
  • Saci Short Description And Applications

    1. 1. Surface Activated Chemical Ionization – applications to low molecular weight drugs analysis Simone Cristoni
    2. 2. Surface Activated Chemical Ionization
    3. 3. Surface Activated Chemical Ionization Controlled, low potential Metal surface
    4. 4. SACI <ul><li>High sensitivity </li></ul><ul><li>High Versatility (different eluent flow rates, compounds and matrix) ‏ </li></ul><ul><li>Cost effective (avoid pre purification step working by sample dilution) ‏ </li></ul>
    5. 5. Analysis of addictive drugs <ul><li>Urine </li></ul><ul><li>Hair </li></ul><ul><li>Saliva </li></ul><ul><li>Sample dilution 1:10 </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography
    6. 6. Analysis of addictive drugs <ul><li>Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisition mode: tandem mass spectrometry (MS/MS) ‏ </li></ul><ul><li>Chromatography column: C18 50x4.1 1.8 µm i.d. </li></ul><ul><li>Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA </li></ul><ul><li>Eluent flow: 500 µL/min </li></ul><ul><li>Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes, from 40 to 5 % in 2 minutes. </li></ul><ul><li>Sample volume injected: 20 µL </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography Experimental conditions
    7. 7. Analysis of addictive drugs Urine 2 ng/mL APCI SACI
    8. 8. Analysis of addictive drugs Saliva 10 ng/mL of 6-MAM 1 ng/mL of 6-MAM 4 Retention time (min)‏ 14 a) SACI‏ Signal intensity HCT 0 5.3E+5 12.40 LTQ 4 Retention time (min)‏ 14 a) SACI‏ Signal intensity HCT 0 4.1E+4 LTQ 4 0 Signal intensity 3.2E+2 Retention time (min)‏ 14 b) APCI HCT LTQ Signal intensity 0 1.89E+2 Retention time (min)‏ 14 c) ESI‏ 4 HCT LTQ 0 Signal intensity 5.6E+2 Retention time (min)‏ 14 b) APCI 4 HCT LTQ Signal intensity 4.2E+3 Retention time (min)‏ 14 c) ESI‏ 0 4 LTQ HCT 12.20 12.30
    9. 9. Analysis of pesticides <ul><li>Water </li></ul><ul><li>Spinach </li></ul><ul><li>Sample dilution 1:10 </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography Different matrix
    10. 10. Analysis of pesticides <ul><li>Mass spectrometer: ESI-TOF (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisition mode: High mass accuracy full scan MS </li></ul><ul><li>Chromatography column: C18 50x4.1 3,5 µm i.d. </li></ul><ul><li>Eluents: A:H2O + 0.1% FA %B :CH3OH + 0.1% FA </li></ul><ul><li>Eluent flow: 500 µL/min </li></ul><ul><li>Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes, from 40 to 5 % in 2 minutes. </li></ul><ul><li>Sample volume injected: 20 µL </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography Experimental conditions
    11. 11. Analysis of pesticides water
    12. 12. Analysis of pesticides spinach
    13. 13. Analysis of toxin in food <ul><li>Wine </li></ul><ul><li>Grape </li></ul><ul><li>Sample dilution 1:10 </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography Different matrix
    14. 14. Analysis of pesticides <ul><li>Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) ‏ </li></ul><ul><li>Acquisition mode: tandem mass spectrometry (MS 3 ) ‏ </li></ul><ul><li>Chromatography column: C18 250x4 5 µm i.d. </li></ul><ul><li>Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA </li></ul><ul><li>Eluent flow: 1mL/min. </li></ul><ul><li>Gradient: %B: 5 for 5 minutes, from 5 to 70 % in 10 minutes, from 70 to 5 % in 2 minutes. </li></ul><ul><li>Sample volume injected: 20 µL </li></ul>Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow gradient chromatography Experimental conditions
    15. 15. Analysis of pesticides spinach
    16. 16. Analysis of OTA Wine – 10 ng/mL
    17. 17. Conclusions <ul><li>High sensitivity (LOD, LOQ, linerity ranges) ‏ </li></ul><ul><li>Cost effecting avoiding matrix effect and pre-concentration step (e.g. Solid Phase Extraction) ‏ </li></ul><ul><li>Possible to use it in a wide range of chromatographic conditions. </li></ul><ul><li>High throughput screening of complex mixture if coupled with high mass accuracy analyzers (e.g TOF or FTICT). </li></ul>
    18. 18. Future developments <ul><li>Ion exchange chromatography (low operative voltages employed in SACI makes possible to operate in these chromatographic conditions). </li></ul><ul><li>High sensitive quantitation analysis of peptide and protein drugs (ioni monocarica; TOF). </li></ul><ul><li>Pesticides (developing high sensitive methods for screening of selected groups of pesticides of interest) ‏ </li></ul>
    19. 19. SACI demo site Web site: http://www.isbiotechnology.com
    20. 20. acknowledgment <ul><li>Dr.ssa Sara Crotti – ISB Milan </li></ul><ul><li>Dr. Lorenzo Zingaro – ISB Milan </li></ul><ul><li>Dr.ssa Cristina Canton – ISB Milan </li></ul><ul><li>Dr.ssa Laura Molin – ISB Milan </li></ul><ul><li>Dr. Pietro Traldi – CNR Padova </li></ul><ul><li>Prof. Luigi Rossi Bernardi – University of Milan </li></ul><ul><li>Dr. Simone Rubini – Bruker Daltonics </li></ul><ul><li>Dr. Elisa Basso – Bruker Daltonics </li></ul><ul><li>Dr. Leopoldo Dimiziano – Bruker Daltonics </li></ul>
    21. 21. www.bdal.com

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