Early stage cancer diagnosis is now possible thanks to DIBIP approach.

1. ISB Ion Source & Biotechnologies Via Fantoli 16/15 (Milano) www.isbiotechnology.com Mail to : info@isbiotechnology.com Director : Dr. Simone Cristoni DIBIP DynamIc BIomarker Profile Early stage cancer diagnosis is possible now 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5

2. ISB Ion Source & Biotechnologies Via Fantoli 16/15 (Milano) www.isbiotechnology.com Mail to : info@isbiotechnology.com Director : Dr. Simone Cristoni Open source Early stage cancer diagnosis through metabolic profiling 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5

3. Apparatus for early stage cancer Diagnsosis DynamIc Biomarker Profile (DIBIP) + LC-MS USIS + OnLine DIBIP Data elaboration system References 1) Cristoni S. et al. Rapid Commun Mass Spectrom. 2009 Sep;23(17):2839-45 2) Cristoni S. PCT International patent n. (WO 2007/131682)

5. Plasma from control subjects Plasma from unknown subjects Plasma from unhealthy patients DIBIP data DIBIP data Control library DIBIP data DIAGNOSIS DIBIP diagnosis data elaboration flow Control library Unhealthy library

6. Results 94 % of Correct diagnosis!!!!!!! Sensitivity: 94% Selectivity: 94%

7. Results Elaboration platform will be made freely available at: http://www.isbiotechnology.com/

8. ISB Ion Source & Biotechnologies Team Dr. Simone Cristoni General Manager Dr. Lorenzo Zingaro Laboratory Manager Dr. Cristina Canton Biologist Dr. Alessandro Finiguerra Chemist Dr. Ombretta Repetto Biologist Dr. Vincenza Flora Dibari Bioinformatician Dr. Martina Soldaini Marketing Dr. Pablo Martínez-Lozano Marie Curie researcher 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5 909.4676 1481.8099 1492.7842 1681.8397 1995.1389 2298.3763 2495.2791 2704.3640 2837.3934 3501.0325 3519.8990 3761.1424 1000 1500 2000 2500 3000 3500 T 16 T 12 T 5 T7 T 1 T 5