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  • 1. NANO BIOREMEDIATION Presented by- Shreya M. Modi, M.Phil/P.hD Student in Nanosience
  • 2. Content Introduction Bioremediation Nanobioremediation Applications Conclusion 2
  • 3. INTRODUCTION• Nanoscience is the study of atoms, Molecules, and objects whose size is on nanometer scale. 3
  • 4. What Is Nanotechnology?Ability to understand, create, and use structures, devices and systems that have fundamentally new properties and functions because of their Nano scale structureAbility to image, measure, model, and Nano area Electron Diffraction manipulate matter on the Nano scale to of DW Carbon Nanotube – Zuo, et.al exploit those properties and functionsAbility to integrate those properties and functions into systems spanning from nano- to macro-scopic scales 4 Corral of Fe Atoms – D. Eigler
  • 5. Components 5
  • 6. 6
  • 7. 7
  • 8. BIOREMEDIATION• The degradation of environmental waste using microorganisms is known as BIOREMEDIATION. 8
  • 9. Biodegradable Contaminants Micro organisms Convert them toGrow on toxic nontoxic compounds products 9
  • 10. Classes of Contaminants Classes of Contaminants Chlorinated ToxicAromatics solvents Oxygenases metals 10
  • 11. NANOBIOREMEDIATION• The use of Nanoscience, Nanoparticals and Nanotechnology to enhance the microbial activity to remove pollutants. Coast NBR Clean up Contaminants time 11
  • 12. GENETIC ENGINEERING OF MICROBES• Nanotechnology and its tools play very important role in genetic engineering of microbes. 12
  • 13. Wide Physiological Diversity Controlled Micro SmallCulturability organisms Size Genetic Manipulability 13
  • 14. http://www.sciencedirect.com/science/article/pii/S0378111911000916 14
  • 15. Deinococcus radiodurans World’s Strongest Bacteria 15
  • 16. Mer A LOCUS of E.coli. 16
  • 17. Conti………• Deinococcus radiodurans, which naturally reduces Cr(VI) to the less mobile and less toxic Cr(III), was engineered for complete toluene degradation by cloned expression of tod and xyl genes of Pseudomonas putida. http://ars.els-cdn.com/content/image/1-s2.0-S0168165600003679-gr3.gif 17
  • 18. NANOSCALE BIOPOLYMERSNanoscale modified biopolymers which can bemanufactured by genetic and protein engineering ofmicroorganisms which can control the size andarrangement at the molecular level. 18
  • 19. DECOLORIZATION OF THE DYE CONGOREDBY Aspergillus niger SILVER NANOPARTICALSSEM image of the Aspergillus niger AgNP 19
  • 20. % of decolorizationof the dye Congo red byAspergillusniger silver nanoparticle and Aspergillus niger (plain culture) 20
  • 21. REMOVAL OF PHENOLIC POLLUTANTS FROM MUNICIPAL WASTE WATER BY IMMOBILIZED LACCASE ENZYMES USING NANOPARTICALSEndocrine disrupting compounds In the present work laccase of a Thielavia genus has been immobilized on fumed silica nanoparticles. Mesoporous carbon materials, have been considered as highly suitable candidates for laccase enzyme isolated from Trametes versicolor and molecule immobilization . 21
  • 22. Degradation of Hydrophobic Compound Enhanced by Nanoparticals• Polymeric nanoparticals prepared from a poly(ethylene) glycol Modified Urethane Acrylite(PMUA) precursor was applied to enhance the bioavailability of Polynuclear Aeromatic Hydrocarbons(PAHs) in soil and aqueus solutions.• Due to the hydrophobicity of interior regions of PMUA there is increased affinity between PAHs and released into the aqueous phase and enhances the rate of Mineralization. 22
  • 23. NANOTECH COATING CAN ENHANCE ELECTRICITY OUTPUT FROM WASTE WATER• In this technology, bacteria from biowaste such as sewage are placed in an anode chamber, where they form a biofilm, consume nutrients and grow, in the process releasing electrons. In this context, the sewage is literally the fuel for electricity production. 23
  • 24. They found that coating graphite anodes with a nanoparticle layer of gold can increase electricityproduction by 20 times, while coatings with palladium produced an increase as well, but not nearly as much 24
  • 25. • Silver nanoparticles (Ag-NPs) in particular, provide effective growth inhibition of various microorganisms in suspension and on solid medium.• In addition, a few types of filtration membranes and devices like catheter incorporating silver nps have demonstrated anti-biofouling properties. 25
  • 26. NANOBIOREMEDIATION TO CLEAN UP OIL SPILL• Immobilization cells of Ps. mendocina H3, Ps. pseudoalcaligenes H7, Ps. stutzeri H10, Ps.alcaligenes H15, Ps. pseudoalcaligenes H16, Ps. mallei 36K and Micrococcus luteus 37 was demonstrated high sorbtional activity carriers. 26
  • 27. Conti…..• Bioremediation of oil contaminated soil is important that CARBONIZATED SORBENTS itself may sorbs oil drops for further oxidation carbohydrates of oil by microbial cells, to be source of mineral compounds and improve condition of soils. 27
  • 28. IMMOBILIZATION OF MICROBIAL CELLS USING NANOPARTICALS• Magnetic nanoparticals (Fe3O4) were functionalized with ammonium oleate and coated on the surface of Pseudomonas delafieldii.• On application of external magnetic field to the microbial cells, the nanopartical coated cells concentrate on particular site of the reactor wall separating them from the whole solution and enabling recycling of the cells for the treatment of the same compound. http://www.ncbi.nlm.nih.gov/pmc/articles /PMC1183266/figure/f2/ 28
  • 29. Bio desulfurization (BDS) of dibenzothiophene(DBT) was carried out by Rhodococcus erythropolis IGST8 decorated with magnetic Fe3O4nanoparticles, synthesized in-house by a chemical method, with an average size of 45–50 nm 29
  • 30. 30
  • 31. MICROBIAL PRODUCTION OF SELENIUM NANOPARTICLES USED FOR WASTE WATER TREATMENT AND OHER APPLICATIONhttp://www.sciencedirect.com/science/article/pii/S0304389411001014 31
  • 32. CONCLUSION• According to above all application of Nanobioremediation it can be definitely concluded that, Nanoparticals, Nanotechnological instrument play efficient role in the process of Nanobioremediation. By applying the nanobioremediation to environment hazards, it can clean them Faster and Safer than other methods and technology. We can say that, Nanobioremediation Maintain all three criteria. 32
  • 33. Social NMREconomical Biological 33
  • 34. Thank You• Thank you. 34