1.plant tissue culture history
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1.plant tissue culture history Presentation Transcript

  • 1. Cell and TissueCulture
  • 2. T.C.Refers to technique ofgrowing plantcells, tissues, organs, seedsor other plant parts in asterile environment on anutrient medium In vitro
  • 3. History Haberlandt Realized that asepsis was necessary when culturemedia enriched with organic substances metabolized bymicroorganisms In 1898 he cultured single cells on Knop’s salt solution In his cultures the cell were able to synthesize starch as wellas increase in size He failed in his goal to induce cell division
  • 4. HistoryDespite of failures Haberlandt proposed that plant cellsare totipotentTotipotency is ability of individual cells to express thephenotype of whole plant from which it is derivedHaberlandt is regarded as father of plant tissue culture
  • 5. HistoryHanning(1904) Embryo culture of selectedcrucifersRobbins (1922), Kotte In Vitro culture of root tipsLaibach (1925) Use of embryo culture techniquein inter-specific crosses of Linum
  • 6. HistoryWhite (1934-37) Successful culture of tomato rootsimportance of vitamins in growth mediaSnow, Gautheret (1934-1939) Importance of auxinas growth regulatorGautheret, Nobecourt, White (1939) Successfulestablishment of continuously growing cultures.
  • 7. HistoryVan Overbeek (1941) Use of coconut milkcontaining a cell division factor first time in DaturaSkoog and Tsui (1951), Miller et al (1955), In vitrocell differentiation, discovery of KinetinMorel and Martin (1952) Application of micrografting to obtain virus free plants
  • 8. HistoryMuir (1953) Isolation and culture of single cellsfrom plantsSkoog and Miller (1957) Discovery of principle ofhormonal control of the organ formation in tissueculture
  • 9. HistoryReinert and Steward (1958-59) First report onsomatic embryogenesisJones et al (1960) Hanging drop method of cellcultureBergmann (1960) Bergmann cell culturetechnique
  • 10. HistoryE C Cocking (1960) Isolation of protoplasts byenzymatic degradation methodMurashige and Skoog (1962) Development ofMS mediaGuha and Maheshwawari (1964) Production ofFirst haploid plant by anther culture
  • 11. HistoryPower et al (1970) Protoplast fusionTakabe et al (1971)Rgeneration of first plantfrom protoplastCarlson et al (1972) First report on inter-specifichybridization through protoplast fusion