5 8 semfinalb-tsyllabus[1]

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5 8 semfinalb-tsyllabus[1]

  1. 1. V SEMESTER B. E. BIO-TECHNOLOGY W.E.F. SEPTEMBER 2008 Teaching Teaching Examination SchemeSl. Subject Title Dept. (Theory / (Max. Marks) lab)No. Code (Hrs/Week) Theory/ I A Total Practical1 06BT-51 Management and BT/ChE/ 04 100 25 125 Entrepreneurship MBA/ ME/IEM2 06BT-52 Biokinetics & BT/ChE 04 100 25 125 Bioreaction Engg.3 06BT-53 Biosensors & BT/IT/ 04 100 25 125 Bioinstrumentation ML/BM4 06BT-54 Immunotechnology BT 04 100 25 1255 06BT-55 Genetic Engg. & BT 04 100 25 125 Applications6 06BT-56 Bioinformatics BT 04 100 25 1257 06BT-L57 Genetic Engineering BT 03 50 25 75 & Immunotechnology Lab8 06BT-L58 Bioinformatics Lab BT 03 50 25 75 VI SEMESTER B. E. BIO-TECHNOLOGY W.E.F FEBRUARY 2009 Teaching Teaching Examination SchemeSl. No. Subject Title Dept. (Theory / (Max. Marks) lab) Code (Hrs/Week) Theory/ IA Total Practical1 06BT-61 Bioprocess Control BT/ChE 04 100 25 125 & Automation2 06BT-62 Clinical Biotechnology BT 04 100 25 1253 06BT-63 Enzyme Technology BT 04 100 25 125 & Biotransformation4 06BT-64 Genomics and Proteomics BT 04 100 25 1255 06BT-65 Pharmaceutical BT 04 100 25 125 Biotechnology6 06BT-66 Elective – A BT/ChE/ 04 100 25 125 ME/CSE7 06BT-L67 Bioprocess Control BT/ChE 03 50 25 75 & Automation Lab8 06BT-L68 Biokinetics & BT 03 50 25 75 Enzyme Technology Lab Elective A : 06BT-661 Animal BT 06BT-662 Plant BT 06BT-663 Microbial BT 06BT-664 Perl Programming 06BT-665 Transport Phenomena 06BT-666 Bioprocess Equipment Design & CAED 1
  2. 2. VII SEMESTER B. E. BIO-TECHNOLOGY W.E.F. SEPTEMBER 2009 Teaching Teaching Examination SchemeSl. Subject Title Dept. (Theory / (Max. Marks) lab)No. Code (Hrs/Week) Theory/ IA Total Practical1 06BT-71 Economics & Plant BT/ChE 04 100 25 125 Design2 06BT-72 Upstream BT 04 100 25 125 Process Technology3 06BT-73 Downstream BT/ChE 04 100 25 125 Process Technology4 06BT-74 Food Biotechnology BT 04 100 25 1255 06BT-75 Elective – B BT/ChE/ 04 100 25 125 CSE/ISE6 06BT-76 Elective – C BT/ChE/ 04 100 25 125 CSE/ISE7 06BT-L77 Upstream processing BT 03 50 25 75 Lab8 06BT-L78 Downstream BT/ChE 03 50 25 75 processing LabElective B : Elective C :06BT-751 Aqua Culture & Marine BT 06BT-761 Biochips & Micro array Technology06BT-752 Dairy BT 06BT-762 Biomaterials06BT-753 Forensic Science 06BT-763 Health Diagnostics06BT-754 Data structures with C 06BT-764 Fundamentals of OS & DBMS06BT-755 Bioreactor Design Concepts 06BT-765 CAD & MATLAB VIII SEMESTER B. E. BIO-TECHNOLOGY W.E.F FEBRUARY 2010 Teaching Teaching Examination SchemeSl. Subject Code Title Dept. (Theory / (Max. Marks) lab)No. (Hrs/Week) Theory/ IA Total Practical1 06BT-81 Project Management BT/MBA/ 04 100 25 125 & IPR ME/IEM/ ChE2 06BT-82 Bioethics & Biosafety BT 04 100 25 1253 06BT-83 Elective – D BT/ChE/ 04 100 25 125 BM4 06BT-84 Elective – E BT/ChE 04 100 25 1255 06BT-85 Project Work BT 15 100 100 2006 06BT-86 Seminar BT 09 --- 50 50Elective D : Elective E :06BT-831 Nano Bio-Technology 06BT-841 Environmental BT06BT-832 Lab to Industrial Scaling 06BT-842 Metabolic Engineering06BT-833 Protein Engg & Insilico Drug Design 06BT-843 Medical Informatics06BT-834 Biomedical Instrumentation 06BT-844 Tissue Engineering06BT-835 Biomolecular Engineering 06BT-845 Facilitation, Validation & QC 2
  3. 3. V SEMESTER MANAGEMENT & ENTREPRENEURSHIPSub. Code : 06BT-51 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 SYLLABUS TO BE PROVIED BY VTU BIOKINETICS & BIOREACTION ENGINEERINGSub. Code : 06BT-52 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONLaw of mass action and rate equation, definitions and examples of elementary and non-elementary reactions, theories of reaction rate and temperature dependency, analysis ofexperimental reactor data: evaluation of rate equation, integral and differential analysis forconstant volume system. Conceptual numericals. 08 HoursUNIT 2. BIOCHEMICAL EQUILIBRIAEquilibrium in chemically reactive systems (single and multiple reactions), evaluation of reactionequilibrium constant, concentration/conversion data, effect of temperature on equilibrium –derivation of G vs. T relation, application of above concepts to biochemical systems. Conceptualnumericals. 04 HoursUNIT 3. BIOREACTORSDesign equations for homogeneous system: batch, stirred tank and tubular flow reactor, sizecomparison of reactor systems, combination reactor systems. Optimization of output and yieldproblems, Qualitative design for consecutive, parallel and mixed reactions and recycle. Factorsaffecting choice of reactors: optimum yield, conversion, selectivity and reactivity. Conceptualnumericals. 08 HoursUNIT 4. NON-IDEAL BIOREACTORSNon-ideal reactors, residence time distribution studies, pulse and step input response of reactors,RTD’s for CSTR and PFR, calculations of conversions for First order reactions, tanks in seriesand dispersion models. Conceptual numericals. 06 Hours PART BUNIT 5. ENZYME KINETICSEnzyme active site, types of enzyme specificities, enzyme kinetics, initial velocity studies,formation of ES complex, derivation of Michaelis-Menton equation, definition of Km andVmax, Lineweaver-Burk and Eadie-Hofstee plots. Units of enzyme activity, Enzyme inhibition:competitive, uncompetitive and non-competitive; Regulations – allosteric and feed backregulation. Conceptual numericals. 10 HoursUNIT 6. KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATIONPhases of cell growth in batch cultures; simple unstructured kinetic models for microbial growth- Monod model; Growth of Filamentous Organisms. Growth associated (primary) and non- 3
  4. 4. growth associated (secondary) product formation kinetics; Leudeking-Piret models; substrate andproduct inhibition on cell growth and product formation; Conceptual numericals. 05 HoursUNIT 7. METABOLIC STOICHIOMETRY AND ENERGETICSStoichiometry of cell Growth and Product Formation- elemental balances, degrees of reductionof substrate and biomass; available-electron balances; yield coefficients of biomass and productformation; maintenance coefficients. Energetic analysis of microbial growth and productformation - oxygen consumption and heat evolution in aerobic cultures; thermodynamicefficiency of growth. Conceptual numericals. 05 HoursUNIT 8. MEDIA DESIGN AND STERILIZATIONMedium requirements for fermentation processes- Carbon, nitrogen, minerals, vitamins and othercomplex nutrients; oxygen requirements; Medium formulation for optimal growth and productformation - examples of simple and complex media; Thermal death kinetics of microorganisms;Batch and continuous heat – Sterilization of Liquid media; Filter sterilization of liquid media 07 HoursTEXT BOOKSChemical Reaction Engineering by Levenspiel O., John Wiley, 3rd Edition, 2006.Elements of Chemical Reaction Engineering by Fogler, H.S., Prentice Hall, 1986.Bioprocess Engineering by Shuler and Kargi Prentice Hall, 1992.Enzyme Kinetics and Mechanism by Paul F Cook & W W Cleland, Garland Science, 2007REFERENCE BOOKSBioprocess Engineering by Aiba, Humprey & Millis, Academic Press.Biochemical Engineering, James Lee, 1992.Biochemical Engineering Fundamentals by Bailey and Ollis, McGraw Hill (2nd Ed.) 1986.Bioprocess Engineering Principles by Pauline M. Doran, 1995. London.Principles of Biochemistry by Leninger A.L., II Edition, 1993.Enzyme Kinetics by Plowman, McGraw Hill, 1972.Chemical Engineering Kinetics by Smith J.M., McGraw Hill, 3rd Edition, New Delhi,1981.Wolf R. Vieth, Bioprocess Engineering – Kinetics, Mass Transport, Reactors and GeneExpression. A Wiley – Interscience Publication, 1992.Chemical Reactor Analysis and Design by Forment G F and Bischoff K B., John Wiley, 1979.Biocatalytic Membrane Reactor by Drioli, Taylor & Francis, 2005QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. BIOINSTRUMENTATION & BIOSENSORSSub. Code : 06BT-53 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONElectrical quantities and units; functional elements of an instrumentation system; static anddynamic characteristics; principles of analog and digital meters; CRO, energy meters, time andfrequency meters; multimeters. Transducers: Classification, resistive strain gages, RTD, LVDT,Peizoelectric transducers, electromagnetic transducers, optical transducers, transducers forbiomedical applications. Conceptual numericals. 06 Hours 4
  5. 5. UNIT 2. BIOMEDICAL INSTRUMENTATIONThe terminology of medical instrumentation, a review of medical and physiological signals,Principles of EEG, ECG and EMG, PC based Instrumentation, Microcontroller basedInstrumentation, Case study on advanced instrumentation design in Cardiac Mapping.Conceptual numericals. 08 HoursUNIT 3. CARDIAC AND VASCULAR SYSTEMOverview of cardiovascular system, Types of blood pressure sensors, Lumped parametermodeling of a catheter-sensor system, Heart sounds, Cardiac catheterization, Indirectmeasurement of blood pressure, Measuring blood flow rate, Measuring blood volume,Pacemakers, Defibrillators, Cardiac-assist devices, Replacement heart valves – relatedinstrumentation of equipments involved and sensors. Conceptual numericals. 06 HoursUNIT 4. RESPIRATORY SYSTEMModeling the respiratory system, Measuring gas flow rate, Measuring lung volume, Tests ofrespiratory mechanics, Measuring gas concentration, Tests of gas transport, Ventilators,Anesthesia machines, Heart-Lung machine – related instrumentation of equipments involved andsensors. Conceptual numericals. 06 Hours PART BUNIT 5. ANALYTICAL INSTRUMENTS pH meters, Radiometric Devices, Fluorescence Spectrophotometers, Chromatology(chromatographic techniques – GC & HPLC), Electrophoresis, and Lab on a chip - relatedinstrumentation, Validation, Commissioning and Maintenance of all the above equipments.Conceptual numericals. 06 HoursUNIT 6. ASSAY TECHNOLOGIES AND DETECTION METHODSIntroduction; Bioassay Design and Implementation; Radiometric Assays; Scintillation ProximityAssays; Fluorescence methodology to cover all types of fluorescence measurements andinstrumentation; Reporter gene Assay applications; Bio-analytical Applications. Conceptualnumericals. 06 HoursUNIT 7. AUTOMATION AND ROBOTICSIntroduction to Automation, types, LERT classification system, components of a robot, softwaresused in robotics, Barcode technology, objectives, decoding, symbologies used, barcode reader(pen-type, laser type, CCD camera and camera based readers). Conceptual numericals 04 HoursUNIT 8. BIOSENSORSIntroduction to Biosensors: Concepts and applications. Biosensors for personal diabetesmanagement. Microfabricated Sensors and the Commercial Development of Biosensors.Electrochemical sensors, Chemical fibrosensors, Ion-selective FETs, Noninvasive blood-gasmonitoring, Blood-glucose sensors. Noninvasive Biosensors in Clinical Analysis. Applicationsof Biosensor-based instruments to the bioprocess industry. Application of Biosensors toenvironmental samples. Introduction to Biochips and their application to genomics. BIAcore -an optical Biosensor. Conceptual numericals. 10 HoursTEXT BOOKSBioinstrumentation and Biosensors by Donald L Wise, Marcel Dekker Inc. 1991BIOSENSORS by COOPER JM (2004). Oxford Publications.Hand book of Biomedical Instrumentation – R. S. Khandpur, 2nd Edition, TMH, 2003. 5
  6. 6. Biosensors and their applications by Yang Victor C & Ngo That T. 2000.Biosensors – An introduction by Eggins Brain R. 1997.Advances in Laboratory Automation-Robotics by J.R. Strimaitis and J.N. Little, ZymarkCorporation, MA 1991.REFERENCE BOOKSAutomation technologies for genome characterization, John Wiley & Sons, Inc. 1997.Transducers and Instrumentation by Murthy D V S. Prentice Hall, 1995High Throughput Screening, Edited by John. P. Devlin. Published by Marcel Dekker. (1998)Commercial Biosensors by Graham Ramsay, John Wiley & Son, INC. (1998)Introduction to bioanalytical sensors by Alice J Cunningham Newyrok, John Wiley, 1988.Applied biosensors by Doland L Wise (1989).Encyclopedia of Medical devices and Instrumentation – J G Webster – John Wiley 1999Introduction to Biomedical equipment technology – J J Carr, J M Brown, Prentice Hall. 1998.Introduction to Biomedical Engineering by J Enderle, S Blanchard & J Bronzino, Elsevier, 2005.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. IMMUNOTECHNOLOGYSub. Code : 06BT-54 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1.THE IMMUNE SYSTEMIntroduction, cells and organs of the immune system, Primary and secondary Lymphoid organs,antigens, antibodies and their structure, types of immune responses; anatomy of immuneresponse. Classification of immune system - innate and adaptive immunity. 06 HoursUNIT 2. HUMORAL-IMMUNITYB-lymphocytes and their activation; structure and function of immunoglobulins; immunoglobulinclasses and subclasses, idiotypes and anti-idiotypic antibodies, genetic control of antibodyproduction, production of monoclonal and polyclonal antibodies. 08 HoursUNIT 3. CELL-MEDIATED IMMUNITYThymus derived lymphocytes (T cells) - their ontogeny and types, MHC Complex, antigenpresenting cells (APC), mechanisms of T cell activation, macrophages, dendritic cells,langerhans cells, mechanism of phagocytosis, Antigen processing and presentation.06 HoursUNIT 4. IMMUNE REGULATION AND TOLERANCEComplement activation and types and their biological functions, cytokines and their role inimmune response, immunotolerance, Hypersensitivity its types and treatment. 06 Hours PART BUNIT 5. IMMUNOLOGICAL DISORDERAutoimmune disorders and types, pathogenic mechanisms, treatment, experimental models ofauto immune disease, primary and secondary immunodeficiency disorders, mechanism of AIDS,rheumatoid arthritis and allergies. 06 Hours 6
  7. 7. UNIT 6. TRANSPLANTATION IMMUNOLOGYImmunological basis of graft, types of transplantation, mechanism of graft rejection, role of HLAin graft rejection, tissue typing, immunosuppression and immunosuppressive drugs, tumorspecific antigens. 06 HoursUNIT 7. MOLECULAR IMMUNOLOGYVaccines and their types, production of recombinant-DNA vaccines. Catalytic antibodies,application of PCR technology to produce antibodies, immunotherapy with geneticallyengineered antibodies. Brief mention about stem cells and applications to immunology. 06 HoursUNIT 8. IMMUNODIAGNOSISAntigen antibody interaction – Precipitation reactions, Agglutination reactions, Blood typing, A,B, ABO & Rh, principles and applications of ELISA, Radio Immuno Assay (RIA), western blotanalysis, immuno-electrophoresis, Immunofluorescence, chemiluminescence assay. 08 HoursTEXT BOOKSImmunology – an Introduction by Tizard, Thomson 2004.Immunology by J Kubey, WH Freeman. 2003.Immunology & Immunotechnology by Ashim K Chakravarthy, Oxford University Press, 2006.Immundiagnostics by S C Rastogi, New Age International. 1996.REFERENCE BOOKSEssential Immunology by Roitt I. Blackwell Scientific Publications, Oxford, 1991.Molecular Immunology By Benjamini E. 2002.Immunology A short course by Benjamini E. and Leskowitz S. Wiley Liss, NY, 1991.The Immune System by Peter Parham, Garland Science, 2005Understanding Immunology by Peter Wood, Pearson Education, II edition, 2006QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. GENETIC ENGINEERING & APPLICATIONSSub. Code : 06BT-55 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONRole of genes within cells, genetic code, genetic elements that control gene expression, methodof creating recombinant DNA molecules, vectors in recombinant DNA technology, biology andsalient features of vectors, types of vectors – plasmids, cosmids, phages and viruses. 06 HoursUNIT 2. ENZYMES IN GENETIC ENGINEERINGIntroduction. Restriction Endonculeases - Exo & exdo nucleases, classification, mode of action.Enzymes in modification - Polynucleotide phosphorylase, DNase, Methylases, phosphatases,polynucleotide Kinase, Ligases, RNase and their mechansim of action. 06 HoursUNIT 3. NUCLEIC ACID HYBRIDIZATION AND AMPLIFICATIONMethods of nucleic acid detection, polymerase chain reaction (PCR) and its applications,variations in PCR and applications, methods of nucleic acid hybridization, probe and target 7
  8. 8. sequences, Southern and Northern hybridization techniques, nucleic acid mutagenesis in vivo andin vitro. 08 HoursUNIT 4. CONSTRUCTION OF DNA LIBRARIESIsolation and purification of nucleic acids, quantification, storage, Isolation of plasmids,Construction of genomic and cDNA libraries, screening and preservation. 06 Hours PART BUNIT 5. GENE TRANSFER TECHNIQUESGene transfer techniques in plants, animals and microbes – Transformation, electroporation,microprojectile system, liposome mediated transfer, gene gun etc. Agrobacterium-mediated genetransfer in plants – Ti plasmid: structure and functions, Ti plasmid based vectors - advantages.Chloroplast transformation. 08 HoursUNIT 6. TRANSGENIC SCIENCE AND GENETIC IMPROVEMENTTransgenic science in plant improvement, biopharming – plants as bioreactors, transgenic cropsfor increased yield, resistance to biotic and abiotic stresses.Techniques of gene mapping in plants. Marker-assisted selection and breeding for improvement.Transgenic science for animal improvement, biopharming - animals as bioreactors forrecombinant proteins, Gene mapping in farm animals. Marker-assisted selection and geneticimprovement of livestock. 08 HoursUNIT 7. OTHER APPLICATIONSMicrobial biotechnology - Genetic manipulation, engineering microbes for the production ofantibiotics, enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills. 06 HoursUNIT 8. GENE THERAPYIntroduction. Methods of Gene therapy. Gene targeting and silencing. Gene therapy in thetreatment of cancer, SCID, muscular dystrophy, respiratory disease (emphysema) etc.,Challenges in gene therapy. Future of gene therapy. 04 HoursTEXT BOOKSIntroduction to Genetic Engineering by Nicholl. Cambridge Low Price Edition, 2006.Principles of gene manipulation - An introduction to genetic engineering, Old R.W., PrimroseS.B., Blackwell Scientific Publications, 1993.From Genetics to Gene Therapy – the molecular pathology of human disease by David SLatchman, BIOS scientific publishers, 1994.Genes VIII by Benjamin Lewis. Oxford University & Cell Press, 2007.DNA Science by David A Micklos, Greg A Freyer and Dvaid A Crotty, I K International, 2003.REFERENCE BOOKSMolecular Biotechnology: Prnciples and Practices by Channarayappa, 2006, University Press.Genetic Engineering Vol. 1-4 (Williamson Edition)Recombinant DNA by Watson et al., 1983.Vectors by Rodriguer and Denhardt, 1987.Current protocols in molecular biology, Greena Publishing Associates, NY, 1988.Berger S.L. Kimmel A.R. Methods in enzymology, Vol.152, Academic Press, 1987.Molecular cloning Volumes I, II and III. Sambrook J et al (2000). Cold Spring Harbor lab Press. 8
  9. 9. QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. BIOINFORMATICSSub. Code : 06BT-56 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. DATABASES & TOOLSIntroduction to Bioinformatics, Need for informatics tools and exercises, Significance ofdatabases towards informatics projects. The nucleotide and protein sequence Databases:GenBank, DDBJ, EMBL, PIR, Primary and Secondary Databases; Format of databases, Genebank flat file. Protein Data Bank (PDB) flat file; FASTA Format, PIR Format; Structure fileformats, PDBSUM, PDBLite, MMDB, SCOP, Pfam; Database of structure viewers. Specializeddatabases: NCBI, Pubmed, OMIM, Medical databases, KEGG, EST databases; Overview ofother popular tools for bioinformatics exercises. 06 HoursUNIT 2. SEQUENCE ALIGNMENT AND DATABASE SEARCHESIntroduction, The evolutionary basis of sequence alignment, the Modular Nature of proteins,Optional Alignment Methods, Substitution scores, substitution matrices, PAM, BLOSUM, Gappenalties, Statistical significance of Alignments, Database similarity searching, FASTA, BLAST,Low-Complexity Regions, Repetitive Elements. Practical Aspect of Multiple SequenceAlignment, Progressive Alignment Methods, CLUSTALW, Motifs and Patterns, PROSITE,3DPSSM. Hidden Markov Models (HMMs), and Threading methods. Conceptual numericals. 08 HoursUNIT 3. PHYLOGENETIC ANALYSISIntroduction to Phylogenetic analysis, rooted and unrooted trees, Elements of phylogeneticModels, Phylogenetic Data Analysis: Alignment, Substitution Model Building, Tree Building,and Tree Evaluation, Building the Data Model (Alignment), Determining the Substitution Model,Tree - Building Methods, Searching for Trees, Rooting Trees, Evaluating Trees and Data,Phylogenetic softwares (CLUSTALW, PHYLIP etc), Conceptual numericals. 06 HoursUNIT 4. PREDICTIVE METHODSPredictive Methods using Nucleotide sequences: Framework, Masking repetitive DNA,Database searches, Codon Bias Detection, Detecting Functional Sites in the DNA (promoters,transcription factor binding sites, translation initiation sites), Integrated Gene Parsing, findingRNA Genes, Web based tools (GENSCAN, GRAIL, GENEFINDER).Predictive Methods using Protein sequences: Protein Identity based on composition, Physicalproperties Based on sequence, secondary structure and folding classes, specialized structures orfeatures, tertiary structure. Related web based software (JPRED, PROSEC, NNPREDICT,SOPMA). 06 Hours PART BUNIT 5. PLASMID MAPPING AND PRIMER DESIGNRestriction mapping, Utilities, DNA strider, MacVector and OMIGA, gene construction KIT,Vector NTI, Web based tools (MAP, REBASE); Primer design – need for tools, Primer designprograms and software (PRIME3). Conceptual numericals. 04 Hours 9
  10. 10. UNIT 6. GENOME BIOINFORMATICSSequencing methods (qualitative), Bioinformatics tools and automation in Genome Sequencing,analysis of Raw genome sequence data, Utility of EST database in sequencing, Bioinformatics indetection of Polymorphisms, SNPs and their relevance, Bioinformatics tools in microarray dataanalysis, tools for comparative genomics. 04 HoursUNIT 7. MOLECULAR VISUALIZATIONGeneration or Retrieval, Structure Visualization, Conformation Generation. Graphicalrepresentation of molecular structures: small molecules (low molecular weight – peptides,nucleotides, disaccharides, simple drugs molecules) and macromolecules (high molecular weightmolecules - proteins, DNA, RNA, membranes). Usages of visualization software available inpublic domain like VMD, Rasmol, Pymol, SpdbViewer, Chime, Cn3D. Rotameric Structures ofProteins (Conformational Flexibility), Canonical DNA Forms (DNA Sequence Effects).Systematic methods of exploring conformational space. 06 HoursUNIT 8. INSILICO MODELING & DRUG DESIGNScope and applications of insilico modeling in modern biology. Comparative modeling,Constructing an initial model, refining the model, manipulating the model, moleculesuperposition and structural alignment, concept of energy minimization, different types ofinteractions and formulation of force fields. Basic MD algorithm, its limitations, treatment oflong range forces. Molecular modeling in drug discovery, deriving bioactive conformations,molecular docking, quantitative structure-activity relationship (QSAR), deriving thePharmacophoric Pattern, Receptor Mapping, Estimating Biological Activities, Ligand - ReceptorInteractions: Docking, Calculation of Molecular Properties, Energy Calculations (no derivation).Conceptual numericals. 12 HoursTEXT BOOKSBIOINFORMATICS by Andreas D Baxevanis. Wiley Interscience, 1998.BIOINFORMATICS: by David W Mount, cold spring harbor, 2001.Introduction to Bioinformatics by Arthur Lesk, Oxford, 2006.BIOINFORMATICS: Stuart M Brown, NYU Medical Center, NY USA. 2000.Fundamental Concepts of Bioinformatics by D E Krane & M L Raymer, Pearson, 2006.REFERENCE BOOKSComputational methods for macromolecular sequence analysis: R F Doolittle. Academic Press,1996.Computational methods in Molecular Biology. S.L.Salzberg, D B Searls, S Kasif, Elsevier, 1998.BIOINFORMATICS – METHODS AND APPLICATIONS: GENOMICS, PROTEOMICS AND DRUGDISCOVERY BY S C RASTOGI, N MENDIRATTA & P RASTOGI, PHI, 2006.The molecular modeling perspective in drug design by N Claude Cohen, 1996, Academic PressAnalytical Tools for DNA, Genes & Genomes: by Arseni Markoff, New Age, 2007.INTRODUCTION TO BIOINFORMATICS by ANNA TRAMONTANO (2007) TAYLOR &FRANCIS.BIOINFORMATICS by DES HIGGINS & WILLIE TAYLOR (2005) OXFORD.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. 10
  11. 11. GENETIC ENGINEERING & IMMUNOTECHNOLOGY LABORATORYSub. Code : 06BTL-57 I.A Marks : 25Hours/week : 03 Exam Hrs. : 03 Exam Marks : 501. Preparation of DNA for PCR applications- Isolation, purity & quantification2. Introduction to PCR – working of PCR equipment, programming, preparation of reagentsand buffers3. Primer designing using softwares4. Gene/ DNA amplification by random/ specific primers5. Southern hybridization6. Gene Transformation7. Agglutination Technique: Blood group identification8. Bacterial Agglutination Technique-Widal test (Tube / slide agglutination)9. Ouchterlony Double Diffusion (ODD) and Radial Immunodiffusion (RID)10. ELISA11. Countercurrent immunoelectrophoresis (CCIEP)12. Immunoelectrophoresis (IEP)13. Rocket immunoelectrophoresis (RIEP)14. Western blot15. Separation of lymphocytes from peripheral blood.TEXT/REFERENCE BOOKSPrinciples of gene manipulation - An introduction to genetic engineering, Old R.W., PrimroseS.B., Blackwell Scientific Publications, 1993.Genetic Engineering Vol. 1-4 (Williamson Edition)Immunology & Immunotechnology by Ashim K Chakravarthy, Oxford University Press,2006.11Immunodiagnostics by S C Rastogi, New Age International.Genes VIII by Benjamin Lewis. Oxford University & Cell PressCurrent protocols in molecular biology, Greena Publishing Associates, NY, 1988.Berger S.L. Kimmel A.R. Methods in enzymology, Vol.152, Academic Press, 1987.DNA Science by David A Micklos, Greg A Freyer and David A Crotty, I K International, 2003.Molecular cloning Volumes I, II and III. Sambrook J et al (2000). Cold Spring Harbor lab Press.Introduction to Genetic engineering by Sandhya Nair BIOINFORMATICS LABORATORYSub. Code : 06BTL-58 I.A Marks : 25Hours/week : 03 Exam Hrs. : 03 Exam Marks : 501. Bibliographic search from PUBMED, SCIRUS, MEDMINER2. Sequence retrieval from Nucleic acid and Protein databases.3. Sequence (FASTA and BLAST) searches – Retrieval of homologs, paralogs, orthologs, andxenologs4. Pair wise comparison of sequences – Analysis of parameters affecting alignment.5. Multiple alignments of sequences and pattern determination using PROSITE 11
  12. 12. 6. Evolutionary studies / Phylogenetic analysis – Analysis of parameters affecting trees.7. Identification of functional sites in Genes / Genomes.8. Secondary structure prediction of proteins and comparison with PDB.9. Restriction mapping: Analysis of maps for suitable molecular biology experiment.10. Primer Design: Factors affecting primer design.11. PDB structure retrieval and visualization: Analysis of homologous structures.12. Comparative Modeling of homologous sequences and validation of modeled structures.13. Determination of ligand-protein interactions using SPDBV/ LIGPLOT14. Superposition of structures – Calculation of RMSD.15. Docking studies – Analysis of substrate / ligand binding using homologous structures.16. Derivation of pharmacophore patterns for sigma selective ligands.TEXT/REFERENCE BOOKSBIOINFORMATICS by Andreas D Boxevanis. Wiley Interscience, 1998.BIOINFORMATICS by David W Mount, cold spring harbor, 2001.BIOINFORMATICS: A biologists guide to biocomputing and the internet. Stuart M Brown,NYU Medical Center, NY USA. 2000.Analytical Tools for DNA, Genes & Genomes: by Arseni Markoff, New Age, 2007.DISCOVERING GENOMICS, PROTEOMICS & BIOINFORMATICS BY A M CAMPBELL & L J HEYER,PEARSON EDUCATION, 2007Fundamental Concepts of Bioinformatics by D E Krane & M L Raymer, Pearson, 2006.Computational methods in Molecular Biology. S.L.Salzberg, D B Searls, S Kasif, Elsevier, 1998.BIOINFORMATICS – METHODS AND APPLICATIONS: GENOMICS, PROTEOMICS AND DRUGDISCOVERY BY S C RASTOGI, N MENDIRATTA & P RASTOGI, PHI, 2006Introduction to Bioinformatics by Arthur Lesk, Oxford, 2006 12
  13. 13. VI SEMESTER BIOPROCESS CONTROL & AUTOMATIONSub. Code : 06BT-61 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100UNIT 1: INSTRUMENTATIONInstrumentation - principles, Introduction to flow, pressure, temperature and liquid levelmeasurements, measurement of important physico-chemical and biochemical parameters,methods of on-line and off-line biomass estimation, flow injection analysis for measurement ofsubstrates, products and other metabolites. 08 HoursUNIT 2: FIRST ORDER SYSTEMSProcess characteristics, Laplace transforms, first order systems – examples, mercury in glassthermometer, liquid level system, linearization, response of first order system for step, pulse,impulse and sinusoidal changes in input, conceptual numericals. 06 HoursUNIT 3: FIRST ORDER SYSTEMS IN SERIESInteracting and non-interacting systems and their dynamic response to step, pulse and impulseinputs; conceptual numericals. 04HoursUNIT 4: SECOND ORDER SYSTEMSSecond order systems with transfer functions (spring-damper, control valve, U-tube manometer),response of second order system to step, pulse / impulse and sinusoidal input – Over damped,under damped and critically damped condition of second order system, transportation lag. 08 Hours PART BUNIT 5: CONTROLLERS AND FINAL CONTROL ELEMENTSActuators, Positioners, Valve body, Valve plugs, Characteristics of final control elements,controllers – two position control, proportional control, derivative control, integral control, P-I(proportional-integral) control, P-D (proportional- derivative) control, P-I-D (proportional-integral-derivative) control, conceptual numericals. 05 HoursUNIT 6: CLOSED LOOP CONTROL SYSTEMSBlock diagrams for servo and regulatory problems. Transient response of first and second orderprocesses for set point changes and load changes with proportional and PI controllers, conceptualnumericals. 05 hoursUNIT 7: CONTROLLER DESIGN AND STABILITYCriteria for stability, Routh test; Root locus (basics), Introduction to frequency response, Bodecriteria for stability, Nyquist criteria; Conceptual numericals. 10 HoursUNIT 8: BIOPROCESSES DYNAMICS AND CONTROLDynamics and control of bioreactors & sterilizers. On-line data analysis for state and parameterestimation techniques for biochemical processes. 06 HoursTEXT BOOKSProcess System analysis and Control by Donald R Coughanowr, 2nd Edition,. McGraw-Hill,1991.Chemical Process Control by George Stephanopoulos, Prentice-Hall of India, 1999. 13
  14. 14. REFERENCE BOOKSProcess dynamics and control by D E Seborg, T F Edger, John Wiley, 1989Process Control by Wayne C. Bequette, Pearson Education Asia.Essentials of Process Control, Luyben and Luyben.Process Modeling, Simulation and Control, William Luyben.Biochemical Engineering Fundamentals by Bailey and Ollis, Mcgraw Hill (2nd Ed.). 1986.Bioprocess Engineering by Shule and Kargi Prentice Hall, 1992.Bioprocess Engineering Principles by Pauline M. Doran, 1995.Wankat P.C. Rate controlled separations, Elsevier, 1990.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. CLINICAL BIOTECHNOLOGYSub. Code : 06BT-62 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONThe philosophy behind and organization of clinical research. Disease target identification andselection, receptor-based approaches, agonists, antagonists, enzyme inhibitors, includinggenomics, proteomic methods for lead optimization and candidate selection of molecules forexploratory human investigation. 06 HoursUNIT 2. CLINICAL PHARMACOLOGYPre-clinical development to support testing in humans: In vitro and in vivo testing of newcompounds-preclinical & clinical trials. Relationship between animal and human pharmacology.Safety testing – acute, sub acute toxicology, genotoxicology, reproductive toxicology, topicalirritation and hypersensitivity, safety pharmacology, immunotoxicology. PharmaceuticalDevelopment - formulations, manufacture and supply of materials, labeling and presentation,stability and storage, purity, compatibility, disposal; Concepts of pharmacovigilance.08 HoursUNIT 3. THERAPEUTICSClinical importance of Therapeutic Proteins: Therapeutic Antibodies and Enzymes; Hormonesand Growth Factors used as therapeutics (erythropoietin & insulin as examples). Interferons,Interleukins and additional Regulatory Factors. Preservation and clinical use of blood and bloodcomponents, principles and safety guide lines for blood transfusion. 05 HoursUNIT 4. MANAGEMENT OF DRUGSManagement of common acute and chronic diseases. Major drug classes including biologicals.Drug effects: Quantification of drug effects, adverse drug reactions (short term & long term),benefits and risks. Population-based drug prescriptions: e.g. children, elderly, pregnant andbreast feeding women, patients with renal or hepatic impairment. Drug Monitoring:Restrictively-used drugs and drug dependence, Overdose and treatment of drug toxicity. Patientcompliance and patient records 07 Hours 14
  15. 15. PART BUNIT 5. STEM CELLS IN HEALTH CAREIntroduction to Stem Cell Biology, Fate Mapping of Stem Cells, Brief introduction into thedifferent types of stem cells. Use of stem cells in therapy of neurological, hematopoetic, hepatic,pancreatic disorders. Applications of epidermal stem cell in Tissue engineering. HematopoieticStem Cells, Classification and clinical manifestations of hematopoietic stem cell disorders. . 07 HoursUNIT 6. HEALTHCARE MARKETPLACEPrinciples and practice of marketing: Marketing structure and competition, Price negotiations,National and local formularies. Product information (Generic v/s Rx), advertising and claims,Product support and promotion Product life-cycle management, Product liability Codes ofpractice (including the MHRA Blue Guide Measurement of healthcare), governmental policy andthird-party reimbursement. Principles of health economics Pharmacoepidemiology Competition,in-licensing, co-marketing. 07 HoursUNIT 7. SOCIAL, ETHICAL AND LEGAL ISSUESIPR: patents and copyrights, (with emphasis on patenting of new active substances). Genetic &sex discrimination: insurance and employment, human cloning, foeticide. Ethical principles withregard to: somatic and germ line gene therapy, Role of ethics committees. Biosafety containmentfacilities, biohazards, genetically modified organisms (GMOs) & living modified organisms(LMOs). 06 HoursUNIT 8. CLINICAL RESEARCHGeneral principles and guide to data sources, types of epidemiology study designs, ecological(correlation) studies, case reports, prevalence surveys or cross-sectional studies, case controlstudies, Clinical trials-informed consent, Placebo Responses, Clinical Registries. ClinicalResearch Institutes, Data Management, Clinical Research from Pharmaceutical IndustryPerspective. 06 HoursTEXT BOOKSBiochemistry and Biotechnology by Gary Walsh. (2002): John Wiley & Sons Ltd.Principles and Practice of Clinical Research by J. I. Gallin and F. P. Ognibene, 2nd Edition,Elsevier Publication, 2007Hematology, William J. Williams, Ernest Beutler, Allan JU. Erslev, Marshall A. Lichtman.Stem Cell Biology by Marshak, 2001, Cold Spring Harbour Symposium Pulblications.Current Trends in Pharmacology by Arunabha Ray & Kavitha Gulati, IK Intl, 2007.REFERENCES BOOKSDevelopmental Biology, 6th Edition, Scott F. Gilbert.Molecular Biology of the Cell, 3rd Edition, Bruce Alberts, Dennis Bray, Julian Lewis, MartinRaff, Keith Roberts, James D. Watson.Text book of Medical Biochemistry by R L Nath, New Age, 2002.Pharmaceutical Biotechnology by K Sambamurthy & Ashutosh Kar, New Age, 2006.Basic & Clinical Pharmacology (2004) 9th Edition by Bartram G. Katzung, Mc Graw HillQUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. 15
  16. 16. ENZYME TECHNOLOGY & BIOTRANSFORMATIONSub. Code : 06BT-63 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONIntroduction to enzymes, Classification, Sources, Mechanism of enzyme action. Strategies ofpurification of enzymes, criteria of purity, molecular weight determination and characterizationof enzymes. 06 HoursUNIT 2. BIOCATALYSTSAdvantages of enzymes vs chemical catalysts, Isolated Enzymes versus whole cell systems,enzymes in fermentation, Biocatalytic Application, Enzyme catalysis (Acid-base, Covalent,Metal ion catalysis, Substrate strain & entropy effects) Mechanism of coenzymes (NAD/NADP,FAD/FADH2, PLP, Coenzyme A, TPP, Biotin) 08 HoursUNIT 3. ENZYMES OF BIOLOGICAL IMPORTANCEAcetylcholinesterase, angiotensin converting enzyme (ACE), ACE Inhibitors, HMG Co Areductase inhibitors, pseudocholinesterase, 5-nucleotidase (5NT), glucose-6-phosphatedehydrogenase (GPD), CKisoforms, immunoreactive trypsinogen (IRT) and chymotrypsin;amylase isoenzymes, 06 HoursUNIT 4. ENZYMATIC TECHNIQUESEnzyme and isoenzyme measurement methods with two examples (fixed incubation and kineticmethods); Methods for investigating the kinetics of Enzyme catalysed reactions – Initial velocitystudies, rapid-reaction techniques. Standardization and optimization methods, stability ofenzymes. 06 Hours PART BUNIT 5. IMMOBILIZED ENZYMESTechniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute,partition & diffusion on the kinetics of immobilized enzymes, design and configuration ofimmobilized enzyme reactors; applications of immobilized enzyme technology, Economicargument for immobilization. 08 HoursUNIT 6. ENZYMATIC TRANSFORMATIONReaction engineering for enzyme-catalyzed biotransformations. Catalytic antibodies.Biocatalysts from extreme Thermophilic and Hyperthermophilic microorganisms(extremozymes). Peptide Synthesis. The design and construction of novel enzymes, artificialenzymes, Biotransformation of drugs (hydroxylation of Steroids), Host Guest Complexationchemistry, enzyme design using steroid templates. 06 HoursUNIT 7. MEDICAL APPLICATIONSImportance of enzymes in diagnostics, Enzyme pattern in diseases like Myocardial infarctions(SGOT, SGPT & LDH). Isoenzymes (CK, LD, ALP). Use of isozymes as markers in cancer andother diseases. Enzymes in immunoassay techniques. Therapeutic enzymes. 06 HoursUNIT 8. INDUSTRIAL APPLICATIONSEnzymes used in detergents, use of proteases in food, leather and wool industries; methodsinvolved in production of glucose syrup from starch (using starch hydrolyzing enzymes),production of maltose and sucrose, glucose from cellulose, uses of lactase in dairy industry,glucose oxidase and catalase in food industry; Restriction enzymes and DNA ligases. 06 Hours 16
  17. 17. TEXT BOOKSFundaments of Enzymology by Nicholas C Price and Stevens Oxford Press. (1999).Enzymes – Biochemistry, Biotechnology, Clinical Chemistry By Trevor Palmer.Biotransformations in organic synthesis By Faber.Enzymes in Industry: Production and Applications, W. Gerhartz (1990), VCH Publishers, NYEnzyme Technology by M.F. Chaplin and C. Bucke, CUP, Cambridge, 1990REFERENCE BOOKSEnzyme Technology by Messing.Purifying Proteins for Proteomics by Richard J Simpson, IK International, 2004Proteins and Proteomics by Richard J Simpson, IK International, 2003Enzymes: Dixon and Webb. IRL Press.Principles of Enzymology for technological Applications (1993): Butterworth Heinemann Ltd.Oxford.Biocatalyst for Industry: J.S. Dordrick (1991), Plenum press, New york.Enzymes in Industry: Production and Applications, W. Gerhartz (1990), VCH Publishers, NY.Principles of Enzymology for technological Applications (1993): Butterworth Heinemann Ltd.Oxford.Biocatalyst for Industry: J.S. Dordrick (1991), Plenum press, New york.Fundaments of Enzymology by Prices and Stevens Oxford Press. (1999).QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. GENOMICS & PROTEOMICSSub. Code : 06BT-64 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONGenes and Proteins, Polymorphisms – types of polymorphism, genome sequences and databasesubscriptions, discovery of new genes and their function. 04 HoursUNIT 2. SEQUENCING & GENOME PROJECTSEarly sequencing efforts. Methods of preparing genomic DNA for sequencing, DNA sequenceanalysis methods, Sanger Dideoxy method, Fluorescence method, shot-gun approach. Genomeprojects on E.coli., Arabidopsis and rice; Human genome project and the genetic map. 06 HoursUNIT 3. GENOMICSGene variation and Single Nucleotide Polymorphisms (SNPs), Expressed sequenced tags (ESTs),Gene-disease association, diagnostic genes and drug targets, genotyping tools - DNA Chips,diagnostic assays, diagnostic services; comparative genomics. Functional genomic studies withmodel systems such as Drosophila, Yeast or C. elegans. 08 HoursUNIT 4. GENOME MANAGEMENT IN EUKARYOTESCell differentiation and gene regulation. Inheritance pattern in eukaryotes, Mutations,organization of eukaryotic genome within the nucleus, chloroplast and mitochondria. Regulationof transcription, transcription factors and the co-ordination of gene expression, translation andpost-translational modification in eukaryotes. Interference RNA, RNA silencing, SiRNA,Applications in Functional genomics, Medicine and Gene Knockdown. 08 Hours 17
  18. 18. PART BUNIT 5. STRUCTURAL GENOMICSC-Values of genomes. General architecture of prokarytoc and eukaryotic genome. Organization ofmitochondrial and chloroplast genome. 04 HoursUNIT 6. GENOME ANALYSISGenetic and physical maps: Breeding requirements for mapping. Molecular markers - RFLP,RAPD, AFLP, microsatellites and SNPs. Methods of molecular mapping, Marker assistedselection. Map-based cloning, T-DNA and transposon tagging. Differential display via RT-PCR.Micro-array in functional genomics. Bioinformatics analysis – clustering methods. Approachesto Physical mapping, FISH - DNA amplification markers; Telomerase as molecular markers.Genome mapping approaches for microorganisms. 07 HoursUNIT 7. PROTEOMICSIntroduction to proteins, Methods of protein isolation, purification, quantification, Large scalepreparation of proteins, use of peptides as probes. Proteomics databases, proteins as drugs;Proteome functional information, two hybrid interaction screens. 05 HoursUNIT 8. PROTEOME ANALYSISMass-spec based analysis of protein expression and post-translational modifications. "ProteinChip" - interactions and detection techniques. Methods of measurement of mRNA expression,DNA array hybridization, Non-DNA array hybridization, Two dimensional PAGE for proteomeanalysis, Image analysis of 2D gels, High throughput proteome analysis by stable isotopelabeling, Automation in proteomics, Applications of proteome analysis to drug development andtoxicology, Phage antibodies as tools for proteomics, Glycoanalysis in proteomics, Proteomics astool for plant genetics and plant breeding. 10 HoursTEXT BOOKSIntroduction to Genomics by Arthur M Lesk, Oxford University Press, 2007.Plant Genome Analysis. Edited by Peter M Gresshoff, CRC Press.Genetic Analysis – Principles, Scope and Objectives by JRS Finchman, Blackwell Science,1994.DISCOVERING GENOMICS, PROTEOMICS & BIOINFORMATICS BY A M CAMPBELL & L J HEYER,PEARSON EDUCATION, 2007Protein Arrays, Biochips and Proteomics by J S Albala & I Humprey-Smith, CRC Press, 2003Genomics & Proteomics by Sabesan, Ane Books, 2007.Proteomics by S R Pennington and M J Dunn, 2004.Purifying Proteins for Proteomics by Richard J Simpson, IK International, 2004Proteins and Proteomics by Richard J Simpson, IK International, 2003REFERENCE BOOKSDNA sequencing by Luhe Alphey, 2004Biocomputing Informatics and the Genome Projects by Smith D.W., Academic Press,1993.Genes VIII by Benjamin Lewis. Oxford University & Cell Press.BIOINFORMATICS – METHODS AND APPLICATIONS: GENOMICS, PROTEOMICS AND DRUGDISCOVERY BY S C RASTOGI, N MENDIRATTA & P RASTOGI, PHI, 2006Genomics and evolution of Eukaryotes by Laura Katz and D Bhattacharya, 2007.QUESTION PAPER PATTERN 18
  19. 19. For every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. PHARMACEUTICAL BIOTECHNOLOGYSub. Code : 06BT-65 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONIntroduction to pharma industry, Biotechnology and Drug design: development and economics,Preclinical studies & principles/practices of process development, early proof-of-concept ofbiological/chemical entities Orphan drugs. Provisions for and use of unlicensed medicines. Drugabuse and dependence, Prescription and Non-prescription drugs. 07 HoursUNIT 2. DRUG METABOLISMEvolution of Drug Metabolism Phase I Metabolism (microsomal oxidation, hydroxylation,dealkylation) Phase II Metabolism (Drug conjugation pathway) CYP Families.Pharmacodynamics and Pharmacokinetics of protein based drugs. Physiologic PharmacokineticModel, Mean Residence Time and Statistical Moment Theory Molecular Mechanism of DrugAction. 07 HoursUNIT 3. TOXICOLOGYBasic concepts, mechanism of action of toxins, biotransformation of toxins & their clearancefrom the body; Toxic intermediates. 04 HoursUNIT 4. DRUG MANUFACTURE AND FORMULATIONBasic concepts and applications, composition, preparation, physicochemical considerations inmanufacture of current biotech products & herbal medicines. Quality control, storage andstability of biotech products. Concept & testing of preformulations & their parameters. Tablets:compressed, granulation, coatings, pills, capsules. Parental preparations, herbal extracts, Oralliquids, Ointments. 08 Hours PART BUNIT 5. DRUG DELIVERY SYSTEMSAdvanced Sustained Release, Advanced drug Delivery Systems: Liposomes and Nanoparticles,biodegradable drug delivery system (hydrogel based). 04 HoursUNIT 6. ANALYSIS OF PHARMACEUTICALSValidation techniques for pharmaceutical industries, Pilot Plant, Scale-Up Techniques.Analytical methods and tests for various drugs & packaging techniques- Glass containers, plasticcontainers, film wrapper, bottle seals; Quality assurance and control. 08 HoursUNIT 7. ADVANCED PHARMACOLOGYIntroduction to pharmaceutical chemistry, classification of drugs based on therapeutic actionsusing suitable examples. Antineoplastic agents, Immunomodulators, Heavy metals and heavymetal antagonists, Therapeutic gases. Free radical biology and antioxidants. 06 HoursUNIT 8. PHARMACOTHERAPYSpecial emphasis on Vitamins, cold remedies, laxatives, analgesics, non-steroidal contraceptives,external antiseptics, antacids, antibiotics, biologicals, herbal products. Pharmacotherapy ofmigraine, Alzheimers, cancer, TB, diabetes and male sexual dysfuntion. Hormone replacementtherapy. Advances and promises of Gene Therapy in combating diseases wherein cure presentlyunknown. 08 Hours 19
  20. 20. TEXT BOOKSAn Introduction to Synthetic Drugs (1980) by Singh & Rangnekar, Himalaya publishing House.Biopharmaceuticals, Biochemistry and Biotechnology by Gary Walsh, Wiley Pub, II ed 2003.Principles of Medicinal Chemistry by Foye.Industrial Pharmaceutical Biotechnology by Heinrich Klefenz, Wiley-VCH edition.Biopharmaceutical Drug Design and Development by S Wu-Pong, Y Rojanasakul, and JRobinson.Pharmaceutical Biotechnology by K Sambamurthy & Ashutosh Kar, New Age, 2006.Pharmaceutical Biotechnology by S P Vyas and V K Dixit, CBS Publishers, 2007REFERENCE BOOKSBasic & Clinical Pharmacology (2004) 9th Edition by Bartram G. Katzung, Mc Graw HillThe Theory & Practice of Industrial Pharmacy (1987) 3rd Edition by Leon Lachman, Herbert A.Lieberman & Joseph & Kanig, Vergese Publishing House BombayEnzyme Technologies for pharmaceutical and biotechnological applications by Herbert A Kirst,Wu-Kuang Yeh, Milton J.Current Trends in Pharmacology by Arunabha Ray & Kavitha Gulati, IK Intl, 2007.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. ELECTIVE A ANIMAL BTSub. Code : 06BT-661 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONHistory and development of animal tissue culture. Equipment and materials (culture vessels, CO2incubator, inverted microscope, cell counters). Principles of sterile techniques. Sources oftissues, types of tissues - epithelial, muscle, connective, nerve and blood. Introduction tobalanced salt solutions. Cell culture media - components of the medium, physical, chemical andmetabolic functions of media. Role of serum and supplements, serum-free media, features andspecifications of MEM, DMEM, RPMI and Ham’s medium. Role of antibiotics in media. 08 HoursUNIT 2. TECHNIQUESMeasurement of cell number - hemocytometer, coulter counter. Measurement of cell viabilityand cytotoxicity. Dye exclusion and inclusion tests, colonigenic assay, macromolecularestimation, MTT based assay. Measuring parameters of growth – growth curves, PDT, Platingefficiency and factors influencing growth. 05 HoursUNIT 3. CELL LINESPrimary culture – Mechanical and enzymatic mode of desegregation, establishment of primaryculture. Subculture - passage number, split ratio, seeding efficiency, criteria for subculture. Celllines - definite and continuous cell lines, characterization, authentication, maintenance andpreservation of cell lines. Contamination - bacterial, viral, fungal and mycoplasmacontaminations, detection and control, cell transformation – normal v/s. transformed cells, 20
  21. 21. growth characteristics of transformed cells. Viral and chemical-mediated methods of cellimmortalization. 08 HoursUNIT 4. CELL CULTUREScale-up of animal cell culture – Factors to be considered. Scale-up of suspension cultures -Batch reactor, continuous culture, perfusion systems. Scale-up of monolayer cultures – rollerbottles, Nunc cell factory, microcarrier cultures, organotypic culture, matrices, factors affectingculture and perspectives. 05 Hours PART BUNIT 5. INVITRO FERTILIZATION & CLONINGConventional methods of animal improvement, predominantly selective breeding and cross-breeding. Embryo biotechniques for augumentation of reproductive efficiency and fastermultiplication of superior germ plasm. Super ovulation Oestrus synchronization. Embryocollection, evaluation and transfer. Invitro maturation of oocytes. Invitro fertilisation and embryoculture. Embryo preservation. Micro manipulation and cloning. Artificial insemination,preparation of foster mother, surgical and non-surgical methods of embryo transfer, donor andrecipient aftercare. Cloning - concept of nuclear transfer, nuclear reprogramming and creation ofDolly. Stem cells - embryonic and adult stem cells, plasticity and concept of regenerativemedicine. 10 HoursUNIT 6. HUMAN GENOMEHuman genome - complexicity of the genome, outlines of human genome project, human diseasegenes. Molecular biological techniques for rapid diagnosis of genetic diseases. Chemicalcarcinogenesis, transfection, oncogenes and antioncogenes. Cryo preservation and transport ofanimal germ plasm (i.e. semen, ovum and embryos). Genetherapy - ex vivo and in vivo genetherapy methods, applications. 06 HoursUNIT 7. TRANSGENICSTansgenic animals - retroviral, microinjection, and engineered embryonic stem cell method oftransgenesis. Application of transgenic animals - biopharming, disease models, functionalknockouts. 04 HoursUNIT 8. OTHER APPLICATIONSApplication of animal cell culture - Vaccine production, specialized cell types. Concepts oftissue engineering - skin, liver, kidney, bladder and heart. Principles and species suitable foraquaculture (Indian major carps and prawns). Genetic status of culture stocks. Chromosomemanipulations - Production of all male and sterile populations, Hypophysation in fishes andprawns. Pearl culture - pearl producing mollusks, rearing of oysters, nucleation for pearlformation and harvesting of pearls. Probiotics and their significance in aquaculture. Moleculartools for the identification of diseases in aquatic species. 06 HoursTEXT BOOKSCulture of Animal Cells, (3rd Edn) R Ian Fredhney. Wiley-LissAnimal Cell Biotechnology, 1990- Spier, RE and Griffith, JB Academic Press, LondonAnimal Biotechnology by Murray Moo-Young (1989), Pergamon Press, OxfordAnimal Cell Technology, Principles and practices, 1987, Butter, M Oxford pressMolecular Biotechnology by Primrose.AN INTRODUCTION TO MOLECULAR BIOTECHNOLOGY by MICHAEL WINK (2006),WILEY 21
  22. 22. REFERENCE BOOKSMethods in Cell Biology, Vol. 57, Animal Cell Culture Methods Ed. JP Mather and D Bames.Academic PressFish and Fisheries India VG JhingramLiving resources for Biotechnology, Animal cells by A. Doyle, R. Hay andB.E. Kirsop (1990),cambridge University Press, cambridge.Animal Cell Culture – Practical Approach, Ed. John RW. Masters, OxfordAnimal Cell Culture Techniques Ed Martin Clynes, SpringerCell Culture Lab Fax. Eds. M Butler & M Dawson, Bios Scientific Publications Ltd. OxfordQUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. PLANT BTSub. Code : 06BT-662 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100UNIT 1. INTRODUCTIONIntroduction to cell and tissue culture. Tissue culture media (composition and preparation).Organogenesis, somatic embryogenesis. Embyo culture. Androgenesis and gynogenesis.Endosperm culture. Protoplast culture and selection of cybrids. Cryopreservation. 04 HoursUNIT 2. PLANT GENETIC ENGINEERINGInduction to Plant Genetic Engineering: Types of plant vectors and their use - Particlebombardment, electroporation, microinjection. Agrobacterium mediated transformation –Technique and applications. Ti and Ri-plasmids as vectors. Screening and selection oftransformants – PCR and hybridization methods. Viruses as a tool to delivery foreign DNA.Transformation of monoctos. Mechanism of transgene interaction - Transgene stability and genesilencing. Generation and maintenance of transgenic plants. 08 HoursUNIT 3. PLANTS FOR BIOTIC STRESSESIntroduction to biotic stresses, types. Application of plant transformation – bt genes, Structureand function of Cry proteins – mechanism of action, critical evaluation. Non-bt like proteaseinhibitors, alpha amylase inhibitor, Transgenic technology for development of virus, bacterialand fungal resistance plants. 06 HoursUNIT 4. IMPROVEMENT OF VARIETIESAbiotic stress – Introduction to drought and salinity stresses, transgenic strategies fordevelopment of drought resistant plants, case studies. Post-harvest losses, long shelf life of fruitsand flowers, use of ACC synthase, polygalacturanase, ACC oxidase, male sterile lines, barstarand barnase systems. Herbicide resistance - phosphoinothricin, glyphosate, atrazine; insectresistance. Biosafety regulations and evaluation of transgenics contained conditions. Implicationsof gene patents 06 Hours 22
  23. 23. PART BUNIT 5. MOLECULAR FARMING AND APPLICATIONSPlant metabolic engineering and industrial products: Molecular farming for the production ofindustrial enzymes, biodegradable plastics, polyhydroxybutyrate, antibodies, edible vaccines.Metabolic engineering of plants for the production of fatty acids, industrial oils, flavonoids etc.,Engineering of carotenoid and provitamin biosynthetic pathways. 06 HoursUNIT 6. NITROGEN FIXATION AND APPLICATIONSNitrogen fixation and biofertilizers - Diazotrophic microorganisms, nitrogen fixation genes. Twocomponent regulatory mechanisms. Transfer of nif genes and nod genes – structure, function androle in nodulation; Hydrogenase - Hydrogen metabolism. Genetic engineering of hydrogenasegenes. 06 HoursUNIT 7. SIGNAL TRANSUCTION IN PLANTSSignal transduction in plants: Mechanism, plant hormone signaling, transduction, lightperception and signaling network in higher plants, calcium signaling, sphingolipids in plantsingling, other molecules. 06 HoursUNIT 8. ALGAL TECHNOLOGIESBlue-green algae and Azolla - Identification of elite species and mass production for practicalapplication. Mycorrhizae - importance in agriculture and forestry. Algae as a source of food,feed, single cell protein, biofertilizers; industrial uses of algae. Mass cultivation of commerciallyvaluable marine macroalgae for agar agar, alginates and other products of commerce and theiruses. Mass cultivation of microalgae as a source of protein and feed. 08 HoursTEXT BOOKSPlant Cell Culture : A Practical Approach by R.A. Dixon & Gonzales, IRL Press.Plant biotechnology in Agriculture by K. Lindsey and M.G.K. Jones (1990), Prentice hall, NewJersey.Plant Biotechnology 1994, Prakash and Perk, Oxford & IBH Publishers CoJ Hammond, P McGarvey and V Yusibov (Eds): Plant Biotechnology. Springer Verlag, 2000HS Chawla: Biotechnology in Crop Improvement. Intl Book Distributing Company, 1998RJ Henry: Practical Application of Plant Molecular Biology. Chapman and Hall 1997REFERENCE BOOKSPlant Tissue Culture: Applications and Limitations by S.S. Bhojwani (1990), Elsevier,Amsterdam.TJ Fu, G Singh and WR Curtis (Eds): Plant Cell and Tissue Culture for the Production of FoodIngredients. Kluwer Academic Press, 1999.PK Gupta: Elements of Biotechnology. Rastogi and Co. Meerut 1996.Biotechnology in Agriculture , MS Swamynathan, McMillian India Ltd.Gene Transfer to Plants (1995) Polyykus I and Spongernberg, G.Ed. Springer Scam.Genetic Engineering with Plant Viruses, 1992 T Michael, A Wilson and JW Davis, CRC Press.Molecular Approaches to Crop Improvement 1991. Dennis Liwelly Eds.Plant Cell and Tissue Culture- A Laboratory mannual 1994. Reinert J and Yeoman MM,Springer.PLANT TISSUE CULTURE by SATHYANARAYANA BN (2007) IK INTL PUBLISHERSMolecular Biotechnology: Prnciples and Practices by Channarayappa, 2006, University Press. 23
  24. 24. QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. MICROBIAL BTSub. Code : 06BT-663 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART - AUNIT 1. INTRODUCTIONStudy of Prokaryotes & Eukaryotes, Classification and Identification of Microorganisms,classification and identification of fungi. 02 HoursUNIT 2. MICROBIAL PROCESS ENGINEERINGIntroduction to microbial process development. Analysis of experimental data. Design &optimization of fermentation media. Kinetics of cell growth. Sterilization of air and media.Modes of cell culture. Bioreactor systems including utilities. Mass transfer in Microbialprocesses. Scale - up of microbial processes. Instrumentation and control of process parameters. 08 HoursUNIT 3. MICROBIAL BIOTECHNOLOGYa) In BacteriaGenetic Transfer in bacteria, Transformation, Conjugation, Transduction, cloning techniques,polymerase chain reaction, expression of cloned Genes, Recovery and purification of expressedproteins. 03 Hoursb) In YeastIntroduction of DNA into yeast cells, yeast cloning vectors, expression of foreign genes in yeast,expression of foreign gene products in secreted form. 03 HoursUNIT 4. INDUSTRIAL MICROBIOLOGYIndustrial production of Vitamins, Antibiotics, organic acids and hormones. Impact ofBiotechnology on vaccine development; sub unit vaccines, fragments of antigen sub unit assynthetic peptide vaccines. Bacillus thuringinesis, Sphaericus, Popilliae, BaculovirusesProduction of Microbial enzymes, strain, medium, fermentation processes. Large scaleapplication of Microbial enzymes - starch processing, textile designing, detergents, cheeseindustry, production of glucose isomerase. 10 Hours PART – BUNIT 5. MICROBIAL BY PRODUCTS.Bacterial Polysaccharides – structure & role in nature xanthan Gum - structure, production &Biosynthesis polyesters. Saccharification & fermentation. Organic synthesis & Degradation ofmicrobial byproducts, microbial transformation of steroids & sterols. 06 HoursUNIT 6. ENVIRONMENTAL MICROBIOLOGYContamination in air, water and soil, Waster water microbiology, Microbiological Degradationof xenobiotics. 02 HoursUNIT 7. BIOREMEDIATION AND BIOLEACHINGUses of Bacteria in Bioremediation – Biodegradation of hydrocarbons, Granular sludge consortiafor bioremediation, crude oil degradation by bacteria, Immobilization of microbes for 24
  25. 25. bioremediation, Methanotrophs, PCB dechlorination, Genetic engineering of microbes forbioremediation. Phytoremediation – plants capable of assimilating heavy metals. Studies ofPyrite Dissolution in Pachuca Tanks and Depression of Pyrite Flotation by Bacteria, Factorsaffecting Microbial Coal Solubilization, Sulfur Leaching by Thermophilic Microbes of CoalParticles Varying in size, Microbiological Production of Ferric Ion for Heap and DumpLeaching, New Bacteriophage which infects Acidophilic, Heterotrophic Bacteria from AcidicMining Environments, Treatment of Coal Mine Drainage with Constructed Wetlands. 12 HoursUNIT 8. FOOD MICROBIOLOGYMicrobial spoilage of food and its control; food preservatives; fermented foods; single cellprotein (SCP) and single cell oil (SCO); food borne infections and their control. 06 HoursTEXT BOOKSFundamentals of Biotechnology. Edited by Paule Prave, Uwe Faust, Wolfgang Sitting and DieterA Sukatsch. VCH Publishers.Principles of fermentation Technology, P.F. Stanbury and A. Whitaker, Pergamon Press, 1984.Alexander N Glazer, Hiroshi Nikaido by Microbial Biotechnology, W H Freeman & CompanyNewyork.REFERENCE BOOKSMicrobiology by Bernard Davis & Renato Dulbecco, Lippincott Company, Philadelphia.Principle of Microbe & Cell Cultivation (1975), SJ Prit, Blackwell Scientific co.).QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. PERL PROGRAMMINGSub. Code : 06BT-664 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. INTRODUCTIONAn overview of Perl: Getting started, interpreted vs compiled source code, documentation inperl, statement blocks, ASCII and Unicode, Escape sequences, whitespaces, numerical data type,strings in perl, alternative delimiters, conversion between numbers and strings, Arithmeticaloperators, bitwise operators, Boolean operators, string operators, string comparison, operatorprecedence, variables, modifying a variable, autoincrement and autodecrement operators,multiple assignments, scoping, special variables, regular expression variables, input/ outputvariables, filehandle / format variables, error variables and system variables variableinterpolation. 08 HoursUNIT 2. LISTS, ARRAYS AND HASHESIntroduction to lists, simple lists, complex lists, accessing list values, list slices, ranges,combining ranges and slices, arrays, assigning arrays, scalar vs list context, adding elements toan array, accessing single and multiple elements from an array, running through arrays, arrayfunctions (pop, push, shift, unshift, and sort); Introduction to Hashes, creating a hash, workingwith hash values, adding, changing and taking values from a hash, accessing multiple values. 06 Hours 25
  26. 26. UNIT 3. LOOPS AND DECISIONSIntroduction, Changing Array Size, Interacting Over an Array by Reference, Extracting UniqueElements from a List, Computing Union, Intersection, or Difference of Unique Lists, AppendingOne Array to Another, Reversing an Array, Processing Multiple Elements of an Array, FindingAll Elements in an Array Matching Certain Criteria, Sorting an Array Numerically. 06 HoursUNIT 4. REGULAR EXPRESSIONIntroduction to regular expressions, patterns, interpolation, escaping special characters, anchors,character classes, word boundaries, posix and Unicode classes, detecting repeating words, well-defined repetition, back reference variables, match operator, substitution operator andtransliteration operator, binding operators, meta-characters, changing delimiters, modifiers,usage of split and join keywords, inline comments and modifiers, grouping and alternation,grouping with back references. 06 Hours PART BUNIT 5. FILES AND REFERENCESIntroduction to Filehandle, STDIN, STDOUT, STDERR file handles, reading lines, creatingfilters, line separator, reading paragraphs, reading entire files, writing to files, writing on a filehandle, accessing filehandle, writing binary data, selecting a filehandle, buffering, filepermissions, opening pipes, piping in, piping out, file tests, reading directories and globbing,introduction to references, lifecycle of a reference, anonymous reference, dereferencing,reference modification, array and hash referencing, reference counting and destruction. 08 HoursUNIT 6. SUBROUTINES AND MODULESIntroduction to subroutines, difference between subroutines and modules, defining subroutines,order of declaration, subroutines for calculations, return values, caching, context, subroutineprototypes, scope, global variables, lexical variables, runtime scope, aliases, passing references ,arrays, hashes and filehandles to a subroutine, modules, usage of keywords do, require and use,changing @INC, package hierarchies, exporters, standard modules in perl. 06 HoursUNIT 7. RUNNING AND DEBUGGING PERLExamining syntax errors, runaway strings, brackets around conditions, missing semicolons,braces, commas and barewords. Diagnostic modules, use warnings, scope of warnings, use strict,strict on variables, references, subroutines, use diagnostics, perl command line switches, usage of–e, -n, -p, -c, -I, -M, -s, -I, @INC, -a, -F and –T switches, Debugging techniques, usage of print,comments, context, scope and precedence in debugging, Defensive programming. 06 HoursUNIT 8. BIOPERLOverview, Bioperl Objects, Brief descriptions (Seq, PrimarySeq, LocatableSeq, RelSegment,LiveSeq, LargeSeq, RichSeq, SeqWithQuality, SeqI), Location objects, Interface objects andimplementation objects, Representing large sequences (LargeSeq), Representing changingsequences (LiveSeq), Using Bioperl: Accessing sequence data from local and remote databases,Accessing remote databases (Bio::DB::GenBank, etc), Indexing and accessing local databasesBio::Index::*, bp_index.pl, bp_fetch.pl, Bio::DB::*), Transforming sequence files (SeqIO),Transforming alignment files (AlignIO); 06 HoursTEXT BOOKSSimon Cozens, Peter Wainwrigth, Beginning Perl, Wrox press, 1st edition , 2000.Tom Christiansen and Nathan Torkinton, Perl cook book, O’Reilly & Associates, USA, 1998.Programming Perl (III edition) by Larry Wall, Tom Christiansen, Jon Orwant, 2000. 26
  27. 27. Learning Perl (III edition) by Randal L, Schwartz, Tom Phoenix, 2001.REFERENCE BOOKSPerl by Example by Ellie Quigley Prentice Hall.Perl in a Nutshell by O’Reilley.Perl: The programmer Companion by Nigel Chapman, Wiley.BioPerl by O’Reilly & Associates.Bioperl from Beginning Perl for Bioinformatics by James Tisdall.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. TRANSPORT PHENOMENASub. Code : 06BT-665 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1. MOMENTUM TRANSFER AND OVERALL BALANCESFluid Statics, General molecular transport equations for momentum, heat and mass transfer,Viscosity of fluids, Overall balances: mass balance/continuity equation, energy balance,momentum balance, shell momentum balance and velocity distribution in laminar flow, designequation for laminar and turbulent flow in pipes, compressible flow of gases. 06 HoursUNIT 2. MOMENTUM TRANSFER – PRINCIPLES AND APPLICATIONSFlow past immersed objects, packed and fluidized beds, Non-Newtonian fluids, Differentialequations of continuity, momentum transfer (motion), use of these equations, other solutionmethods for differential equation of motion, boundary layer flow and turbulence, dimensionalanalysis in momentum transfer. 08 HoursUNIT 3. STEADY STATE HEAT TRANFERMechanisms of heat transfer, conduction – through solids in series, steady state conduction andshape factors, Forced convection - heat transfer inside pipes, heat transfer outside variousgeometries, natural convection heat transfer, boiling and condensation, heat exchangers,radiation heat transfer (basic and advanced), heat transfer to non-Newtonian fluids, special heattransfer coefficients, dimensional analysis in heat transfer, numerical methods for steady stateheat transfer in two dimensions. 06 HoursUNIT 4. UNSTEADY STATE HEAT TRANSFERDerivation of basic equation, simplified case for systems with negligible internal resistance,unsteady state heat transfer in various geometries, finite difference methods, chilling andfreezing of food and biological materials, differential equation of energy change, boundary layerflow and turbulence in heat transfer. 06 Hours PART BUNIT 5. MASS TRANSFERMass transfer and diffusion, molecular diffusion in gases, liquids, biological solutions and gels,and solids, numerical methods for steady state molecular diffusion in two dimensions. 06 HoursUNIT 6. UNSTEADY STATE AND CONVECTIVE MASS TRANSFERUnsteady state diffusion, convective mass transfer coefficients, for various geometries, masstransfer to suspensions of small particle, molecular diffusion plus convection and chemical 27
  28. 28. reaction, diffusion of gases in porous solids and capillaries, numerical methods for unsteady statemolecular diffusion, dimensional analysis in mass transfer, boundary layer flow and turbulencein heat transfer. 09 HoursUNIT 7. SEPARATION PROCESSES-1Evaporation, Drying, Humidification, Absorption, Distillation. 05 HoursUNIT 8. SEPARATION PROCESSES-2Adsorption, Ion Exchange, Leaching, Crystallization, Membrane processes, Settling,Centrifugation and Size Reduction. 06 HoursTEXT BOOKTransport Processes and Separation Process Principles, C. J. Geankoplis, 4th Edition.Momentum, Heat and Mass Transfer, Bennett and Myers.Introduction to Transport Phenomena, William J. Thomson, PHI.Transport Phenomena, Bird, Stewart, Lightfoot, 2nd Edition, JWI.Fundamentals of momentum, heat and mass transfer, Welty, Wicks and Wilson.Fundamentals of FLUID MECHANICS by SAWHNEY GS (2008) IK Publishers.REFERENCE BOOKSUnit Operations of Chemical Engg. by McCabe & Smith (M G H Publications), 6th Edition 1999.Principles of Unit Operations in Chemical Engg. by Geonklopins.Fluid Mechanics by K L Kumar.Mechanics of fluids by B.S. Massey.QUESTION PAPER PATTERNFor every 6-7 Hours of teaching One Question to be Set. Eight questions to be set (four fromeach part) out of which five full questions (considering at least two from each part) to beanswered. BIOPROCESS EQUIPMENT DESIGN AND CAEDSub. Code : 06BT- 666 I.A Marks : 25Hours/week : 04 Exam Hrs. : 04Total Hours : 52 Exam Marks : 100UNIT 1: INTRODUCTION TO DESIGNa) Types of joints (welded)b) Types of pipe fittingsc) Types of valves- ball and safetyUNIT 2: PROCESS EQUIPMENTS DESIGN USING CAEDDetailed process and mechanical design of the following equipmentsa) Agitated and jacketed vesselsb) Fermentor vesselsc) Shell and tube exchangersTEXT BOOKSProcess equipment design, M V Joshi.Unfired pressure vessel I S Code 2825Shell and tube heat exchanger specifications, I S Code 4503Chemical engineers hand book, Perry and Green, 7th Edition, 1997.REFERENCE BOOKSProcess equipment and mechanical aspect, V C Bhattacharya. 28
  29. 29. Mechanical equipment design, Brownell and Young.Fermentation and biochemical engineering hand book. (1983), Principles, process design andequipment. H C Vogel, Noyes.Chemical Engineering, Coulson and Richradson, Vol. 6.Equipment design by Atkins.QUESTION PAPER PATTERNTwo main questions to be set, out of which one full question to be answered BIOPROCESS CONTROL & AUTOMATION LABSub. Code : 06BTL-67 I.A Marks : 25Hours/week : 03 Exam Hrs. : 03 Exam Marks : 50 1. Characteristics of Transducers (Temperature). 2. Characteristics of Transducers (Pressure). 3. Characteristics of Transducers (Flow). 4. Measurement of OD and DO for microbial cultures 5. Dynamics of First order system (mercury thermometer) for step input and impulse input. 6. Non-interacting system responses to step / pulse input 7. Interacting System responses to step / pulse input 8. Temperature controller – responses to set point / load change 9. pH controller – responses to set point / load change 10. Tuning of Flow controller (ZN and CC methods) and responses of tuned P, PI and PID controllers 11. Tuning of Pressure controller (ZN and CC methods) and responses of tuned P, PI and PID controllers 12. Offline measurement of protein quantity and purity using SDS PageTEXT / REFERENCE BOOKSProcess System analysis and Control by Donald R Coughanowr, 2nd Edition,. McGraw-Hill,1991Chemical Process Control by George Stephanopoulos, Prentice-Hall of India.Bioprocess Engineering by Shule and Kargi Prentice Hall, 1992.Bioprocess Engineering Principles by Pauline M. Doran, 1995.Wolf R. Vieth, Bioprocess Engineering – Kinetics, Mass Transport, Reactors and GeneExpression. A Wiley - Interscience Publication, 1992. BIOKINETICS & ENZYME TECHNOLOGY LABSub. Code : 06BTL-68 I.A Marks : 25Hours/week : 03 Exam Hrs. : 03 Exam Marks : 501. Batch Growth Kinetics.2. Mixed Flow Reactor Analysis.3. Plug Flow Reactor Analysis.4. Batch Reactor Analysis5. RTD in PFR6. RTD in MFR 29
  30. 30. 7. Isolation of enzymes (alpha-amylase from sweet potato or saliva, urease from horse gram orkidney gram, acid phosphotase from sweet potato)8. Determination of Enzyme activity and Specific activity.9. Effect of substrate concentration on enzyme activity (Km & Vmax determination)10. Effect of pH on enzyme activity11. Effect of temperature on enzyme activity12. Effect of inhibitors on enzyme activity13. Ammonium sulphate fractionation and desalting (Dialysis/G-25 column chromatography)14. Molecular weight determination of a protein by SDS PAGE / molecular sieving15. Enzyme Immobilization Techniques and Kinetics.TEXT/REFERENCE BOOKSBiochemical Engineering Fundamentals by Bailey and Ollis, Mcgraw Hill (2nd Ed.). 1986.Bioprocess Engineering by Shule and Kargi Prentice Hall, 1992.Wolf R. Vieth, Bioprocess Engineering – Kinetics, Mass Transport, Reactors and GeneExpression. A Wiley – Interscience Publication, 1992.Smith J.M. Chemical Engineering Kinetics, McGraw Hill, 3rd Edition, New Delhi,1981.Carbery J A. Chemical and Catalytic Reactor Engineering, McGraw Hill, 1976.Enzymes in Industry: Production and Applications : W. Gerhartz (1990), VCH Publishers,New York.Enzyme Technology by M.F. Chaplin and C. Bucke, Cambridge University Press, Cambridge,1990.Enzymes: Dixon and Webb. IRL Press.Principles of Enzymology for technological Applications (1993): B Heinemann Ltd. Oxford. 30
  31. 31. VII SEMESTER ECONOMICS & PLANT DESIGNSub. Code : 06BT-71 I.A Marks : 25Hours/week : 04 Exam Hrs. : 03Total Hours : 52 Exam Marks : 100 PART AUNIT 1: PROCESS DESIGN DEVELOPMENTDesign project procedure, design information from the literature and other sources ofinformation, flow diagrams, preliminary design, comparison of different processes, firm processdesign, equipment design and specialization, scale up in design, safety factors specifications,materials of construction. 06 HoursUNIT 2: GENERAL DESIGN CONSIDERATIONSMarketability of the product, availability of technology, raw materials, human resources, landand utilities, site characteristics, plant location, plant layout, plant operation and control, utilities,structural design, storage, materials handling, materials and fabrication selection, optimumdesign and design strategy. Waste disposal, govt. regulations and other legal restrictions,community factors, safety and hazard control measures. 08 HoursUNIT 3: CAPITAL INVESTMENTSFixed capital investments including land, building, equipment and utilities, installation costs,(including equipment, instrumentation, piping, electrical installation and other utilities), workingcapital investments. 06 HoursUNIT 4: MANUFACTURING COSTS AND PLANT OVERHEADSManufacturing Costs: Direct Production costs (including raw materials, human resources,maintenance and repair, operating supplies, power and other utilities, royalties, etc.), fixedcharges (including depreciation, taxes, insurance, rental costs etc.). Plant Overheads:Administration, safety and other auxiliary services, payroll overheads, warehouse and storagefacilities etc. Conceptual numericals. 06 Hours PART BUNIT 5: COST ANALYSIS AND TIME VALUE OF MONEYCost Analysis: Factors involved in project cost estimation, methods employed for the estimationof the capital investment. Estimation of working capital. Time value of money and equivalence.Conceptual numericals. 08 HoursUNIT 6: DEPRECIATION AND TAXESDepreciation calculation methods. Equivalence after Taxes. Cost comparison after taxes.Conceptual numericals. 06 HoursUNIT 7: PROFITABILITY ANALYSISMethods for the evaluation of profitability. Return on original investment, interest rate of return,accounting for uncertainty and variations and future developments. Replacement and AlternativeInvestments. Opportunity costs. Conceptual numericals. 08 HoursUNIT 8: FINANCIAL STATEMENTS AND REPORTSFinancial statements. Cash flow diagrams. Break-even analysis. Design Report: Types of reports.Organization of report . Conceptual numericals. 04 Hours 31

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