Types of centrifuges

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Types of centrifuges

  1. 1. Types of centrifuges
  2. 2. • Depending on the particular application, centrifuges differ in their overall design and size.• a common feature in all centrifuges is the central motor that spins a rotor containing the samples to be separated
  3. 3. Types of Centrifuge depends on :• Maximum speed of sedimentation• Presence /absence of vacuum• Temperature control refrigeration.• Volume of sample andcapacity of centrifugation tubes
  4. 4. 1. Small Benchtop with or without refrigeration slow speed (eg up to 4000 RPM) common in clinical lab (blood/plasma/serum separation) can take approx (up to) 100 tubes, depending on diameter
  5. 5. 2. Microcentrifuges (“microfuge”, “Eppendorf”) take tubes of small vols (up to 2 mL) very common in biochemistry/molecular biology/ biological labs can generate forces up to ~15,000 x g with or without refrigeration
  6. 6. 3.High Speed centrifuges 15,000 – 20,000 RPM centrifugal field of 100,000 g large sample capacity depending on rotor normally refrigerated research applications. Differentiation separation of nucleus, mitochondrial, protein precipitate, large intact organelle, cellular debris, bulky protein aggregates.
  7. 7. 4. Ultracentrifuges 65,000 RPM (100,000’s x g) limited lifetime Expensive require special rotors care in use – balance critical! research applications The high speeds used in such devices generate considerable amounts of heat Therefore cooling arrangements and vaccuum are required in ultracentrifuges
  8. 8. 5. Large-capacity preparative centrifuge• Centrifugal fields of 3000 to 7000g.• Efficient separation of coarse precipitates or whole cells.

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