Plant Propagation Tissue Culture
Tissue Culture <ul><li>A method of growing plants (called explants) on an artificial medium under sterile conditions </li>...
Requirements <ul><li>Proper tissue & plant </li></ul><ul><li>Growth hormones in the media </li></ul><ul><li>Growth medium ...
Types <ul><li>Callus culture </li></ul><ul><ul><li>Grown on a petri dish </li></ul></ul><ul><ul><li>Solid media </li></ul>...
Steps <ul><li>Establish a fern culture in organic media - callus </li></ul><ul><ul><li>Take a cutting, sanitize it, put in...
Sterile environment <ul><li>All surfaces washed with alcohol </li></ul><ul><li>All tools sterilized </li></ul><ul><li>All ...
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Tissue culture

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Tissue culture

  1. 1. Plant Propagation Tissue Culture
  2. 2. Tissue Culture <ul><li>A method of growing plants (called explants) on an artificial medium under sterile conditions </li></ul><ul><li>Very technical </li></ul><ul><li>Requires sterile environment </li></ul><ul><li>Used commercially to mass propagate high dollar plants. </li></ul><ul><ul><li>Orchids, ferns, Japanese maples, Kiwis </li></ul></ul>
  3. 3. Requirements <ul><li>Proper tissue & plant </li></ul><ul><li>Growth hormones in the media </li></ul><ul><li>Growth medium with everything necessary to nourish the plant. </li></ul><ul><li>Sterile environment </li></ul>
  4. 4. Types <ul><li>Callus culture </li></ul><ul><ul><li>Grown on a petri dish </li></ul></ul><ul><ul><li>Solid media </li></ul></ul><ul><ul><li>Callus forms a lump of tissue </li></ul></ul><ul><li>Cell suspension culture </li></ul><ul><ul><li>Grown in a jar </li></ul></ul><ul><ul><li>Liquid media </li></ul></ul><ul><ul><li>Small clusters of cells </li></ul></ul>
  5. 5. Steps <ul><li>Establish a fern culture in organic media - callus </li></ul><ul><ul><li>Take a cutting, sanitize it, put in agar </li></ul></ul><ul><ul><li>16 hrs of florescent light a day </li></ul></ul><ul><ul><li>Wait 4 – 6 weeks </li></ul></ul><ul><li>Cut off any new shoots that have grown </li></ul><ul><li>Place in multiplying media </li></ul><ul><ul><li>Wait 2-4 weeks </li></ul></ul><ul><ul><li>Roots established – plantlets </li></ul></ul><ul><li>Place in growing media </li></ul><ul><ul><li>Keep moist for 1-2 weeks </li></ul></ul>
  6. 6. Sterile environment <ul><li>All surfaces washed with alcohol </li></ul><ul><li>All tools sterilized </li></ul><ul><li>All air is filtered </li></ul><ul><ul><li>Laminar airflow system </li></ul></ul><ul><li>Don’t breathe on media </li></ul>
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